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Self-compatibility in 'Zaohong' Japanese apricot is associated with the loss of function of pollen S genes.

Wang PP, Gao ZH, Ni ZJ, Zhang Z, Cai BH - Mol. Biol. Rep. (2013)

Bottom Line: Furthermore, expression analysis based on RT-PCR of S-locus genes revealed no differences at the transcript level when compared with 'Xiyeqing', a self-incompatible cultivar with the same S haplotypes.Putative structural regions of PmF-box genes have been described, corresponding to regions in PmSFB alleles, but with some sequence variations.These results suggest that SC in 'Zaohong' occurs in pollen, and that other factors outside the S-locus, including PmF-box genes, might be associated with the loss of function of pollen S genes.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Nanjing Agricultural University, No.1 Weigang, Nanjing City, 210095, Jiangsu Province, People's Republic of China.

ABSTRACT
While most Japanese apricot (Prunus mume Sieb. et Zucc.) cultivars display typical S-RNase-based gametophytic self-incompatibility, some self-compatible (SC) cultivars have also been identified. In this study, we confirmed SC of 'Zaohong' through replicated self-pollination tests. Cross-pollination tests showed that SC of 'Zaohong' was caused by a loss of pollen function, so we determined that the S-genotype of 'Zaohong' was S 2 S 15 . Sequence analysis of the S-haplotypes of 'Zaohong' showed no mutations which were likely to alter gene function. Furthermore, expression analysis based on RT-PCR of S-locus genes revealed no differences at the transcript level when compared with 'Xiyeqing', a self-incompatible cultivar with the same S haplotypes. In addition, except for S-locus genes, a new type of F-box gene encoding a previously uncharacterised protein with high sequence similarity (61.03-64.65 %) to Prunus SFB genes was identified. Putative structural regions of PmF-box genes have been described, corresponding to regions in PmSFB alleles, but with some sequence variations. These results suggest that SC in 'Zaohong' occurs in pollen, and that other factors outside the S-locus, including PmF-box genes, might be associated with the loss of function of pollen S genes.

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Phylogenetic tree of 25 SFB/SLF(L) and F-box genes in GSI plants based on aligned amino acid sequences using neighbor-joining. The scale bar represents 0.05 substitutions per amino acid site. The numbers at each branch indicate the percentage of 1,000 bootstrap replicates supporting the grouping at that node. GenBank accession numbers: PaSFB1 (AY805048), PaSFB2 (AY805049), ParSFB1(AY587563), ParSFB11 (EU652884), PdSFBc (AB079776), PdSFBd (AB081648), PpsFB (HM347516), PsSFBb (AB252412), PsSFBc (AB280792), PmSFB1 (AB101440), PmSFB3 (AB376968), PmSFB7 (AB101441), PmSLFL1-S1 (AB092623), PmSLFL1-S7 (AB092624), PmSLFL2-S1 (AB092625), PmSLFL2-S7 (AB092626), PmSLFL3-S7 (AB092627), PdSLFd (AB101660), ParF-box1 (DQ422943), ParF-box2 (DQ422944) and ParF-box3 (DQ422945), the accession numbers of PmSFB2, PmSFB15 and two PmF-box genes were detailed in the text
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Fig5: Phylogenetic tree of 25 SFB/SLF(L) and F-box genes in GSI plants based on aligned amino acid sequences using neighbor-joining. The scale bar represents 0.05 substitutions per amino acid site. The numbers at each branch indicate the percentage of 1,000 bootstrap replicates supporting the grouping at that node. GenBank accession numbers: PaSFB1 (AY805048), PaSFB2 (AY805049), ParSFB1(AY587563), ParSFB11 (EU652884), PdSFBc (AB079776), PdSFBd (AB081648), PpsFB (HM347516), PsSFBb (AB252412), PsSFBc (AB280792), PmSFB1 (AB101440), PmSFB3 (AB376968), PmSFB7 (AB101441), PmSLFL1-S1 (AB092623), PmSLFL1-S7 (AB092624), PmSLFL2-S1 (AB092625), PmSLFL2-S7 (AB092626), PmSLFL3-S7 (AB092627), PdSLFd (AB101660), ParF-box1 (DQ422943), ParF-box2 (DQ422944) and ParF-box3 (DQ422945), the accession numbers of PmSFB2, PmSFB15 and two PmF-box genes were detailed in the text

Mentions: A rooted phylogenetic tree of 25 genes including SFB/SLF(L) and F-box genes in GSI plants was constructed using aligned amino acid sequences by the neighbour-joining method (Fig. 5). Two major classes, the SFB clade and the SLF(L) clade, were found in the phylogenetic tree which demonstrate that SFB/SLF(L) and F-box genes share a common ancestor in Prunus. The tree also suggests that pollen determinant SLF(L) genes likely diverged earlier in S-RNase-based SI evolution.Fig. 5


Self-compatibility in 'Zaohong' Japanese apricot is associated with the loss of function of pollen S genes.

Wang PP, Gao ZH, Ni ZJ, Zhang Z, Cai BH - Mol. Biol. Rep. (2013)

Phylogenetic tree of 25 SFB/SLF(L) and F-box genes in GSI plants based on aligned amino acid sequences using neighbor-joining. The scale bar represents 0.05 substitutions per amino acid site. The numbers at each branch indicate the percentage of 1,000 bootstrap replicates supporting the grouping at that node. GenBank accession numbers: PaSFB1 (AY805048), PaSFB2 (AY805049), ParSFB1(AY587563), ParSFB11 (EU652884), PdSFBc (AB079776), PdSFBd (AB081648), PpsFB (HM347516), PsSFBb (AB252412), PsSFBc (AB280792), PmSFB1 (AB101440), PmSFB3 (AB376968), PmSFB7 (AB101441), PmSLFL1-S1 (AB092623), PmSLFL1-S7 (AB092624), PmSLFL2-S1 (AB092625), PmSLFL2-S7 (AB092626), PmSLFL3-S7 (AB092627), PdSLFd (AB101660), ParF-box1 (DQ422943), ParF-box2 (DQ422944) and ParF-box3 (DQ422945), the accession numbers of PmSFB2, PmSFB15 and two PmF-box genes were detailed in the text
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3824209&req=5

Fig5: Phylogenetic tree of 25 SFB/SLF(L) and F-box genes in GSI plants based on aligned amino acid sequences using neighbor-joining. The scale bar represents 0.05 substitutions per amino acid site. The numbers at each branch indicate the percentage of 1,000 bootstrap replicates supporting the grouping at that node. GenBank accession numbers: PaSFB1 (AY805048), PaSFB2 (AY805049), ParSFB1(AY587563), ParSFB11 (EU652884), PdSFBc (AB079776), PdSFBd (AB081648), PpsFB (HM347516), PsSFBb (AB252412), PsSFBc (AB280792), PmSFB1 (AB101440), PmSFB3 (AB376968), PmSFB7 (AB101441), PmSLFL1-S1 (AB092623), PmSLFL1-S7 (AB092624), PmSLFL2-S1 (AB092625), PmSLFL2-S7 (AB092626), PmSLFL3-S7 (AB092627), PdSLFd (AB101660), ParF-box1 (DQ422943), ParF-box2 (DQ422944) and ParF-box3 (DQ422945), the accession numbers of PmSFB2, PmSFB15 and two PmF-box genes were detailed in the text
Mentions: A rooted phylogenetic tree of 25 genes including SFB/SLF(L) and F-box genes in GSI plants was constructed using aligned amino acid sequences by the neighbour-joining method (Fig. 5). Two major classes, the SFB clade and the SLF(L) clade, were found in the phylogenetic tree which demonstrate that SFB/SLF(L) and F-box genes share a common ancestor in Prunus. The tree also suggests that pollen determinant SLF(L) genes likely diverged earlier in S-RNase-based SI evolution.Fig. 5

Bottom Line: Furthermore, expression analysis based on RT-PCR of S-locus genes revealed no differences at the transcript level when compared with 'Xiyeqing', a self-incompatible cultivar with the same S haplotypes.Putative structural regions of PmF-box genes have been described, corresponding to regions in PmSFB alleles, but with some sequence variations.These results suggest that SC in 'Zaohong' occurs in pollen, and that other factors outside the S-locus, including PmF-box genes, might be associated with the loss of function of pollen S genes.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Nanjing Agricultural University, No.1 Weigang, Nanjing City, 210095, Jiangsu Province, People's Republic of China.

ABSTRACT
While most Japanese apricot (Prunus mume Sieb. et Zucc.) cultivars display typical S-RNase-based gametophytic self-incompatibility, some self-compatible (SC) cultivars have also been identified. In this study, we confirmed SC of 'Zaohong' through replicated self-pollination tests. Cross-pollination tests showed that SC of 'Zaohong' was caused by a loss of pollen function, so we determined that the S-genotype of 'Zaohong' was S 2 S 15 . Sequence analysis of the S-haplotypes of 'Zaohong' showed no mutations which were likely to alter gene function. Furthermore, expression analysis based on RT-PCR of S-locus genes revealed no differences at the transcript level when compared with 'Xiyeqing', a self-incompatible cultivar with the same S haplotypes. In addition, except for S-locus genes, a new type of F-box gene encoding a previously uncharacterised protein with high sequence similarity (61.03-64.65 %) to Prunus SFB genes was identified. Putative structural regions of PmF-box genes have been described, corresponding to regions in PmSFB alleles, but with some sequence variations. These results suggest that SC in 'Zaohong' occurs in pollen, and that other factors outside the S-locus, including PmF-box genes, might be associated with the loss of function of pollen S genes.

Show MeSH