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Self-compatibility in 'Zaohong' Japanese apricot is associated with the loss of function of pollen S genes.

Wang PP, Gao ZH, Ni ZJ, Zhang Z, Cai BH - Mol. Biol. Rep. (2013)

Bottom Line: Furthermore, expression analysis based on RT-PCR of S-locus genes revealed no differences at the transcript level when compared with 'Xiyeqing', a self-incompatible cultivar with the same S haplotypes.Putative structural regions of PmF-box genes have been described, corresponding to regions in PmSFB alleles, but with some sequence variations.These results suggest that SC in 'Zaohong' occurs in pollen, and that other factors outside the S-locus, including PmF-box genes, might be associated with the loss of function of pollen S genes.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Nanjing Agricultural University, No.1 Weigang, Nanjing City, 210095, Jiangsu Province, People's Republic of China.

ABSTRACT
While most Japanese apricot (Prunus mume Sieb. et Zucc.) cultivars display typical S-RNase-based gametophytic self-incompatibility, some self-compatible (SC) cultivars have also been identified. In this study, we confirmed SC of 'Zaohong' through replicated self-pollination tests. Cross-pollination tests showed that SC of 'Zaohong' was caused by a loss of pollen function, so we determined that the S-genotype of 'Zaohong' was S 2 S 15 . Sequence analysis of the S-haplotypes of 'Zaohong' showed no mutations which were likely to alter gene function. Furthermore, expression analysis based on RT-PCR of S-locus genes revealed no differences at the transcript level when compared with 'Xiyeqing', a self-incompatible cultivar with the same S haplotypes. In addition, except for S-locus genes, a new type of F-box gene encoding a previously uncharacterised protein with high sequence similarity (61.03-64.65 %) to Prunus SFB genes was identified. Putative structural regions of PmF-box genes have been described, corresponding to regions in PmSFB alleles, but with some sequence variations. These results suggest that SC in 'Zaohong' occurs in pollen, and that other factors outside the S-locus, including PmF-box genes, might be associated with the loss of function of pollen S genes.

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a Alignment of the predicted amino acid sequences of PmSFB alleles. b Alignment of the predicted amino acid sequences of PmF-box genes, PmSFB1, PmSFB2 and PmSFB15. F-box motif, variable (V1 and V2) and hypervariable (HVa and HVb) regions are boxed. GenBank accession numbers: PmSFB1 (AB101440). PmSFB2 and PmSFB15 were determined in this study. Asterisks, dots and dashes indicate conserved amino acid residues, conservative substitutions and gaps, respectively
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Fig4: a Alignment of the predicted amino acid sequences of PmSFB alleles. b Alignment of the predicted amino acid sequences of PmF-box genes, PmSFB1, PmSFB2 and PmSFB15. F-box motif, variable (V1 and V2) and hypervariable (HVa and HVb) regions are boxed. GenBank accession numbers: PmSFB1 (AB101440). PmSFB2 and PmSFB15 were determined in this study. Asterisks, dots and dashes indicate conserved amino acid residues, conservative substitutions and gaps, respectively

Mentions: To analyse the structure of the PmSFB genes, PCRs were performed using F-BOX5′A and Pm-Vb. As with PmSFB1, PmSFB2 and PmSFB15 of ‘Zaohong’ were found to have an F-box motif and four (hyper-) variable regions (V1, V2, HVa and HVb) (Fig. 4a). Because the corss-pollination tests indicated that SC in ‘Zaohong’ occurred in pollen, the specific expression of SFB genes was tested using RT-PCR. Figure 2 showed that SFB genes of ‘Zaohong’ have the same expression levels in pollen as SFB genes of ‘Xiyeqing’. Taken together, these findings indicate that neither the coding sequence nor the transcriptional level is altered in SFB genes of ‘Zaohong’.Fig. 4


Self-compatibility in 'Zaohong' Japanese apricot is associated with the loss of function of pollen S genes.

Wang PP, Gao ZH, Ni ZJ, Zhang Z, Cai BH - Mol. Biol. Rep. (2013)

a Alignment of the predicted amino acid sequences of PmSFB alleles. b Alignment of the predicted amino acid sequences of PmF-box genes, PmSFB1, PmSFB2 and PmSFB15. F-box motif, variable (V1 and V2) and hypervariable (HVa and HVb) regions are boxed. GenBank accession numbers: PmSFB1 (AB101440). PmSFB2 and PmSFB15 were determined in this study. Asterisks, dots and dashes indicate conserved amino acid residues, conservative substitutions and gaps, respectively
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3824209&req=5

Fig4: a Alignment of the predicted amino acid sequences of PmSFB alleles. b Alignment of the predicted amino acid sequences of PmF-box genes, PmSFB1, PmSFB2 and PmSFB15. F-box motif, variable (V1 and V2) and hypervariable (HVa and HVb) regions are boxed. GenBank accession numbers: PmSFB1 (AB101440). PmSFB2 and PmSFB15 were determined in this study. Asterisks, dots and dashes indicate conserved amino acid residues, conservative substitutions and gaps, respectively
Mentions: To analyse the structure of the PmSFB genes, PCRs were performed using F-BOX5′A and Pm-Vb. As with PmSFB1, PmSFB2 and PmSFB15 of ‘Zaohong’ were found to have an F-box motif and four (hyper-) variable regions (V1, V2, HVa and HVb) (Fig. 4a). Because the corss-pollination tests indicated that SC in ‘Zaohong’ occurred in pollen, the specific expression of SFB genes was tested using RT-PCR. Figure 2 showed that SFB genes of ‘Zaohong’ have the same expression levels in pollen as SFB genes of ‘Xiyeqing’. Taken together, these findings indicate that neither the coding sequence nor the transcriptional level is altered in SFB genes of ‘Zaohong’.Fig. 4

Bottom Line: Furthermore, expression analysis based on RT-PCR of S-locus genes revealed no differences at the transcript level when compared with 'Xiyeqing', a self-incompatible cultivar with the same S haplotypes.Putative structural regions of PmF-box genes have been described, corresponding to regions in PmSFB alleles, but with some sequence variations.These results suggest that SC in 'Zaohong' occurs in pollen, and that other factors outside the S-locus, including PmF-box genes, might be associated with the loss of function of pollen S genes.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Nanjing Agricultural University, No.1 Weigang, Nanjing City, 210095, Jiangsu Province, People's Republic of China.

ABSTRACT
While most Japanese apricot (Prunus mume Sieb. et Zucc.) cultivars display typical S-RNase-based gametophytic self-incompatibility, some self-compatible (SC) cultivars have also been identified. In this study, we confirmed SC of 'Zaohong' through replicated self-pollination tests. Cross-pollination tests showed that SC of 'Zaohong' was caused by a loss of pollen function, so we determined that the S-genotype of 'Zaohong' was S 2 S 15 . Sequence analysis of the S-haplotypes of 'Zaohong' showed no mutations which were likely to alter gene function. Furthermore, expression analysis based on RT-PCR of S-locus genes revealed no differences at the transcript level when compared with 'Xiyeqing', a self-incompatible cultivar with the same S haplotypes. In addition, except for S-locus genes, a new type of F-box gene encoding a previously uncharacterised protein with high sequence similarity (61.03-64.65 %) to Prunus SFB genes was identified. Putative structural regions of PmF-box genes have been described, corresponding to regions in PmSFB alleles, but with some sequence variations. These results suggest that SC in 'Zaohong' occurs in pollen, and that other factors outside the S-locus, including PmF-box genes, might be associated with the loss of function of pollen S genes.

Show MeSH