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Self-compatibility in 'Zaohong' Japanese apricot is associated with the loss of function of pollen S genes.

Wang PP, Gao ZH, Ni ZJ, Zhang Z, Cai BH - Mol. Biol. Rep. (2013)

Bottom Line: Furthermore, expression analysis based on RT-PCR of S-locus genes revealed no differences at the transcript level when compared with 'Xiyeqing', a self-incompatible cultivar with the same S haplotypes.Putative structural regions of PmF-box genes have been described, corresponding to regions in PmSFB alleles, but with some sequence variations.These results suggest that SC in 'Zaohong' occurs in pollen, and that other factors outside the S-locus, including PmF-box genes, might be associated with the loss of function of pollen S genes.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Nanjing Agricultural University, No.1 Weigang, Nanjing City, 210095, Jiangsu Province, People's Republic of China.

ABSTRACT
While most Japanese apricot (Prunus mume Sieb. et Zucc.) cultivars display typical S-RNase-based gametophytic self-incompatibility, some self-compatible (SC) cultivars have also been identified. In this study, we confirmed SC of 'Zaohong' through replicated self-pollination tests. Cross-pollination tests showed that SC of 'Zaohong' was caused by a loss of pollen function, so we determined that the S-genotype of 'Zaohong' was S 2 S 15 . Sequence analysis of the S-haplotypes of 'Zaohong' showed no mutations which were likely to alter gene function. Furthermore, expression analysis based on RT-PCR of S-locus genes revealed no differences at the transcript level when compared with 'Xiyeqing', a self-incompatible cultivar with the same S haplotypes. In addition, except for S-locus genes, a new type of F-box gene encoding a previously uncharacterised protein with high sequence similarity (61.03-64.65 %) to Prunus SFB genes was identified. Putative structural regions of PmF-box genes have been described, corresponding to regions in PmSFB alleles, but with some sequence variations. These results suggest that SC in 'Zaohong' occurs in pollen, and that other factors outside the S-locus, including PmF-box genes, might be associated with the loss of function of pollen S genes.

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Alignment of the predicted amino acid sequences of PmS-RNase alleles. Asterisks, dots and dashes indicate conserved amino acid residues, conservative substitutions and gaps, respectively. Conserved (C1, C2, C3, RC4 and C5) and hypervariable (RHV) regions are boxed, and arrows indicate the position of the introns. GenBank accession numbers: PmS1 (AB364462). PmS2 and PmS15 were determined in this study
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Fig3: Alignment of the predicted amino acid sequences of PmS-RNase alleles. Asterisks, dots and dashes indicate conserved amino acid residues, conservative substitutions and gaps, respectively. Conserved (C1, C2, C3, RC4 and C5) and hypervariable (RHV) regions are boxed, and arrows indicate the position of the introns. GenBank accession numbers: PmS1 (AB364462). PmS2 and PmS15 were determined in this study

Mentions: Using the primer pair, Pru-C2 and PCE-R, we confirmed that the S-genotype of ‘Zaohong’ was S2S15 based on sequence analysis. To analyse further the structure of S-RNase alleles, we cloned PCR products of almost full-length sequences of S-RNase genes that contained the first and second introns, using SRc-F and PM-C5. These primers were designed from the signal peptide and the fifth conserved region of Prunus S-RNase genes, respectively. S2-RNase and S15-RNase were successfully cloned using SRc-F and PM-C5. Compared with the predicted amino acid sequence of PmS1-RNase, the putative S2-RNase and S15-RNase genes showed the typical features of Prunus S-RNase genes with five conserved domains (C1, C2, C3, RC4 and C5) and one RHV (Fig. 3). These results indicated that there were no mutations or insertions in S2-RNase and S15-RNase which could disrupt the typical features.Fig. 3


Self-compatibility in 'Zaohong' Japanese apricot is associated with the loss of function of pollen S genes.

Wang PP, Gao ZH, Ni ZJ, Zhang Z, Cai BH - Mol. Biol. Rep. (2013)

Alignment of the predicted amino acid sequences of PmS-RNase alleles. Asterisks, dots and dashes indicate conserved amino acid residues, conservative substitutions and gaps, respectively. Conserved (C1, C2, C3, RC4 and C5) and hypervariable (RHV) regions are boxed, and arrows indicate the position of the introns. GenBank accession numbers: PmS1 (AB364462). PmS2 and PmS15 were determined in this study
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3824209&req=5

Fig3: Alignment of the predicted amino acid sequences of PmS-RNase alleles. Asterisks, dots and dashes indicate conserved amino acid residues, conservative substitutions and gaps, respectively. Conserved (C1, C2, C3, RC4 and C5) and hypervariable (RHV) regions are boxed, and arrows indicate the position of the introns. GenBank accession numbers: PmS1 (AB364462). PmS2 and PmS15 were determined in this study
Mentions: Using the primer pair, Pru-C2 and PCE-R, we confirmed that the S-genotype of ‘Zaohong’ was S2S15 based on sequence analysis. To analyse further the structure of S-RNase alleles, we cloned PCR products of almost full-length sequences of S-RNase genes that contained the first and second introns, using SRc-F and PM-C5. These primers were designed from the signal peptide and the fifth conserved region of Prunus S-RNase genes, respectively. S2-RNase and S15-RNase were successfully cloned using SRc-F and PM-C5. Compared with the predicted amino acid sequence of PmS1-RNase, the putative S2-RNase and S15-RNase genes showed the typical features of Prunus S-RNase genes with five conserved domains (C1, C2, C3, RC4 and C5) and one RHV (Fig. 3). These results indicated that there were no mutations or insertions in S2-RNase and S15-RNase which could disrupt the typical features.Fig. 3

Bottom Line: Furthermore, expression analysis based on RT-PCR of S-locus genes revealed no differences at the transcript level when compared with 'Xiyeqing', a self-incompatible cultivar with the same S haplotypes.Putative structural regions of PmF-box genes have been described, corresponding to regions in PmSFB alleles, but with some sequence variations.These results suggest that SC in 'Zaohong' occurs in pollen, and that other factors outside the S-locus, including PmF-box genes, might be associated with the loss of function of pollen S genes.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Nanjing Agricultural University, No.1 Weigang, Nanjing City, 210095, Jiangsu Province, People's Republic of China.

ABSTRACT
While most Japanese apricot (Prunus mume Sieb. et Zucc.) cultivars display typical S-RNase-based gametophytic self-incompatibility, some self-compatible (SC) cultivars have also been identified. In this study, we confirmed SC of 'Zaohong' through replicated self-pollination tests. Cross-pollination tests showed that SC of 'Zaohong' was caused by a loss of pollen function, so we determined that the S-genotype of 'Zaohong' was S 2 S 15 . Sequence analysis of the S-haplotypes of 'Zaohong' showed no mutations which were likely to alter gene function. Furthermore, expression analysis based on RT-PCR of S-locus genes revealed no differences at the transcript level when compared with 'Xiyeqing', a self-incompatible cultivar with the same S haplotypes. In addition, except for S-locus genes, a new type of F-box gene encoding a previously uncharacterised protein with high sequence similarity (61.03-64.65 %) to Prunus SFB genes was identified. Putative structural regions of PmF-box genes have been described, corresponding to regions in PmSFB alleles, but with some sequence variations. These results suggest that SC in 'Zaohong' occurs in pollen, and that other factors outside the S-locus, including PmF-box genes, might be associated with the loss of function of pollen S genes.

Show MeSH