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Self-compatibility in 'Zaohong' Japanese apricot is associated with the loss of function of pollen S genes.

Wang PP, Gao ZH, Ni ZJ, Zhang Z, Cai BH - Mol. Biol. Rep. (2013)

Bottom Line: Furthermore, expression analysis based on RT-PCR of S-locus genes revealed no differences at the transcript level when compared with 'Xiyeqing', a self-incompatible cultivar with the same S haplotypes.Putative structural regions of PmF-box genes have been described, corresponding to regions in PmSFB alleles, but with some sequence variations.These results suggest that SC in 'Zaohong' occurs in pollen, and that other factors outside the S-locus, including PmF-box genes, might be associated with the loss of function of pollen S genes.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Nanjing Agricultural University, No.1 Weigang, Nanjing City, 210095, Jiangsu Province, People's Republic of China.

ABSTRACT
While most Japanese apricot (Prunus mume Sieb. et Zucc.) cultivars display typical S-RNase-based gametophytic self-incompatibility, some self-compatible (SC) cultivars have also been identified. In this study, we confirmed SC of 'Zaohong' through replicated self-pollination tests. Cross-pollination tests showed that SC of 'Zaohong' was caused by a loss of pollen function, so we determined that the S-genotype of 'Zaohong' was S 2 S 15 . Sequence analysis of the S-haplotypes of 'Zaohong' showed no mutations which were likely to alter gene function. Furthermore, expression analysis based on RT-PCR of S-locus genes revealed no differences at the transcript level when compared with 'Xiyeqing', a self-incompatible cultivar with the same S haplotypes. In addition, except for S-locus genes, a new type of F-box gene encoding a previously uncharacterised protein with high sequence similarity (61.03-64.65 %) to Prunus SFB genes was identified. Putative structural regions of PmF-box genes have been described, corresponding to regions in PmSFB alleles, but with some sequence variations. These results suggest that SC in 'Zaohong' occurs in pollen, and that other factors outside the S-locus, including PmF-box genes, might be associated with the loss of function of pollen S genes.

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PCR amplification of S-RNase and SFB alleles from ‘Zaohong’ and expression analysis for PmS-RNase (a), PmSFB (b), PmF-box genes (c) and Actin genes (d) in pollen (P), styles (S), and leaves (L). M is DNA marker, Z is Zaohong and G is genomic DNA. a RT-PCR of S-RNase alleles. b RT-PCR of SFB alleles. c RT-PCR of PmF-box alleles. d RT-PCR of Actin genes
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Fig1: PCR amplification of S-RNase and SFB alleles from ‘Zaohong’ and expression analysis for PmS-RNase (a), PmSFB (b), PmF-box genes (c) and Actin genes (d) in pollen (P), styles (S), and leaves (L). M is DNA marker, Z is Zaohong and G is genomic DNA. a RT-PCR of S-RNase alleles. b RT-PCR of SFB alleles. c RT-PCR of PmF-box alleles. d RT-PCR of Actin genes

Mentions: Two DNA bands were obtained from ‘Zaohong’ using the primer pair Pru-C2 and PCE-R (Fig. 1, S-RNase). Further cloning and sequencing confirmed that the S-genotype of ‘Zaohong’ was S2S15, the same as the identification by Xu et al. [32].Fig. 1


Self-compatibility in 'Zaohong' Japanese apricot is associated with the loss of function of pollen S genes.

Wang PP, Gao ZH, Ni ZJ, Zhang Z, Cai BH - Mol. Biol. Rep. (2013)

PCR amplification of S-RNase and SFB alleles from ‘Zaohong’ and expression analysis for PmS-RNase (a), PmSFB (b), PmF-box genes (c) and Actin genes (d) in pollen (P), styles (S), and leaves (L). M is DNA marker, Z is Zaohong and G is genomic DNA. a RT-PCR of S-RNase alleles. b RT-PCR of SFB alleles. c RT-PCR of PmF-box alleles. d RT-PCR of Actin genes
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3824209&req=5

Fig1: PCR amplification of S-RNase and SFB alleles from ‘Zaohong’ and expression analysis for PmS-RNase (a), PmSFB (b), PmF-box genes (c) and Actin genes (d) in pollen (P), styles (S), and leaves (L). M is DNA marker, Z is Zaohong and G is genomic DNA. a RT-PCR of S-RNase alleles. b RT-PCR of SFB alleles. c RT-PCR of PmF-box alleles. d RT-PCR of Actin genes
Mentions: Two DNA bands were obtained from ‘Zaohong’ using the primer pair Pru-C2 and PCE-R (Fig. 1, S-RNase). Further cloning and sequencing confirmed that the S-genotype of ‘Zaohong’ was S2S15, the same as the identification by Xu et al. [32].Fig. 1

Bottom Line: Furthermore, expression analysis based on RT-PCR of S-locus genes revealed no differences at the transcript level when compared with 'Xiyeqing', a self-incompatible cultivar with the same S haplotypes.Putative structural regions of PmF-box genes have been described, corresponding to regions in PmSFB alleles, but with some sequence variations.These results suggest that SC in 'Zaohong' occurs in pollen, and that other factors outside the S-locus, including PmF-box genes, might be associated with the loss of function of pollen S genes.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Nanjing Agricultural University, No.1 Weigang, Nanjing City, 210095, Jiangsu Province, People's Republic of China.

ABSTRACT
While most Japanese apricot (Prunus mume Sieb. et Zucc.) cultivars display typical S-RNase-based gametophytic self-incompatibility, some self-compatible (SC) cultivars have also been identified. In this study, we confirmed SC of 'Zaohong' through replicated self-pollination tests. Cross-pollination tests showed that SC of 'Zaohong' was caused by a loss of pollen function, so we determined that the S-genotype of 'Zaohong' was S 2 S 15 . Sequence analysis of the S-haplotypes of 'Zaohong' showed no mutations which were likely to alter gene function. Furthermore, expression analysis based on RT-PCR of S-locus genes revealed no differences at the transcript level when compared with 'Xiyeqing', a self-incompatible cultivar with the same S haplotypes. In addition, except for S-locus genes, a new type of F-box gene encoding a previously uncharacterised protein with high sequence similarity (61.03-64.65 %) to Prunus SFB genes was identified. Putative structural regions of PmF-box genes have been described, corresponding to regions in PmSFB alleles, but with some sequence variations. These results suggest that SC in 'Zaohong' occurs in pollen, and that other factors outside the S-locus, including PmF-box genes, might be associated with the loss of function of pollen S genes.

Show MeSH