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Arabidopsis CHROMOSOME TRANSMISSION FIDELITY 7 (AtCTF7/ECO1) is required for DNA repair, mitosis and meiosis.

Bolaños-Villegas P, Yang X, Wang HJ, Juan CT, Chuang MH, Makaroff CA, Jauh GY - Plant J. (2013)

Bottom Line: No significant change was observed in the expression of genes that influence entry into the endocycle.Analysis of meiocytes identified changes in chromosome morphology and defective segregation; the abundance of chromosomal-bound cohesion subunits was also reduced.Taken together our results demonstrate that Arabidopsis CTF7/ECO1 plays important roles in the preservation of genome integrity and meiosis.

View Article: PubMed Central - PubMed

Affiliation: Institute of Plant and Microbial Biology, Academia Sinica, Taipei, 11529, Taiwan.

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Related in: MedlinePlus

Leaves of ctf7-1 and ctf7-2 mutant seedlings are defective in mitotic cell division.(a) Cryo-scanning electron microscopy revealed a dramatic increase in cell size of ctf7-1 and ctf7-2 pavement cells (adaxial).(b) Statistical quantification of average pavement cell area.(c) Total cell number per 1 mm2 leaf area.(d) Flow cytometry analyses of leaf cells’ DNA content (‘C’) showed a significant increase (‘4C’) in ctf7-1 and ctf7-2. Data are shown as means ± SD (n = 100 for B and C, and 10 000 for D) from three biological samples. Asterisks represent significant differences (**P < 0.01, ***P < 0.001; Student's t-test) relative to wild type (WT).
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fig07: Leaves of ctf7-1 and ctf7-2 mutant seedlings are defective in mitotic cell division.(a) Cryo-scanning electron microscopy revealed a dramatic increase in cell size of ctf7-1 and ctf7-2 pavement cells (adaxial).(b) Statistical quantification of average pavement cell area.(c) Total cell number per 1 mm2 leaf area.(d) Flow cytometry analyses of leaf cells’ DNA content (‘C’) showed a significant increase (‘4C’) in ctf7-1 and ctf7-2. Data are shown as means ± SD (n = 100 for B and C, and 10 000 for D) from three biological samples. Asterisks represent significant differences (**P < 0.01, ***P < 0.001; Student's t-test) relative to wild type (WT).

Mentions: The extreme dwarf phenotype of ctf7-1 and ctf7-2 plants suggested that, similar to the situation in yeast (Moldovan et al., 2006), Arabidopsis CTF7 plays an important role in cell cycle progression. To determine if ctf7-1 and ctf7-2 cells show cell cycle arrest, we analyzed the morphology and density of pavement and stomata cells in the first true leaves of 7-day-old seedlings by cryo-scanning electron microscopy (cryo-SEM). In WT, pavement and stomata cells were small (Figure 7a), developed at a density of 1070 and 590 cells mm−2 (Figure 7b) and covered an approximate area of 892 μm2 (for pavement cells) and 88.0 μm2 (for stomata) (Figure 7c). Similar values were observed for the ctf7-1 complementation line (Com) (Figure 7a,b). In cells of ctf7-1 and ctf7-2 there was a significant reduction in the density of both pavement cells (580 cells mm−2), and stomata (250 cells mm−2) (Figure 7b), accompanied by a significant increase in the average area of pavement cells (1300 μm2 per cell) (Figure 7c); however, no change was observed in the area of stomatal cells under cryo-SEM.


Arabidopsis CHROMOSOME TRANSMISSION FIDELITY 7 (AtCTF7/ECO1) is required for DNA repair, mitosis and meiosis.

Bolaños-Villegas P, Yang X, Wang HJ, Juan CT, Chuang MH, Makaroff CA, Jauh GY - Plant J. (2013)

Leaves of ctf7-1 and ctf7-2 mutant seedlings are defective in mitotic cell division.(a) Cryo-scanning electron microscopy revealed a dramatic increase in cell size of ctf7-1 and ctf7-2 pavement cells (adaxial).(b) Statistical quantification of average pavement cell area.(c) Total cell number per 1 mm2 leaf area.(d) Flow cytometry analyses of leaf cells’ DNA content (‘C’) showed a significant increase (‘4C’) in ctf7-1 and ctf7-2. Data are shown as means ± SD (n = 100 for B and C, and 10 000 for D) from three biological samples. Asterisks represent significant differences (**P < 0.01, ***P < 0.001; Student's t-test) relative to wild type (WT).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3824207&req=5

fig07: Leaves of ctf7-1 and ctf7-2 mutant seedlings are defective in mitotic cell division.(a) Cryo-scanning electron microscopy revealed a dramatic increase in cell size of ctf7-1 and ctf7-2 pavement cells (adaxial).(b) Statistical quantification of average pavement cell area.(c) Total cell number per 1 mm2 leaf area.(d) Flow cytometry analyses of leaf cells’ DNA content (‘C’) showed a significant increase (‘4C’) in ctf7-1 and ctf7-2. Data are shown as means ± SD (n = 100 for B and C, and 10 000 for D) from three biological samples. Asterisks represent significant differences (**P < 0.01, ***P < 0.001; Student's t-test) relative to wild type (WT).
Mentions: The extreme dwarf phenotype of ctf7-1 and ctf7-2 plants suggested that, similar to the situation in yeast (Moldovan et al., 2006), Arabidopsis CTF7 plays an important role in cell cycle progression. To determine if ctf7-1 and ctf7-2 cells show cell cycle arrest, we analyzed the morphology and density of pavement and stomata cells in the first true leaves of 7-day-old seedlings by cryo-scanning electron microscopy (cryo-SEM). In WT, pavement and stomata cells were small (Figure 7a), developed at a density of 1070 and 590 cells mm−2 (Figure 7b) and covered an approximate area of 892 μm2 (for pavement cells) and 88.0 μm2 (for stomata) (Figure 7c). Similar values were observed for the ctf7-1 complementation line (Com) (Figure 7a,b). In cells of ctf7-1 and ctf7-2 there was a significant reduction in the density of both pavement cells (580 cells mm−2), and stomata (250 cells mm−2) (Figure 7b), accompanied by a significant increase in the average area of pavement cells (1300 μm2 per cell) (Figure 7c); however, no change was observed in the area of stomatal cells under cryo-SEM.

Bottom Line: No significant change was observed in the expression of genes that influence entry into the endocycle.Analysis of meiocytes identified changes in chromosome morphology and defective segregation; the abundance of chromosomal-bound cohesion subunits was also reduced.Taken together our results demonstrate that Arabidopsis CTF7/ECO1 plays important roles in the preservation of genome integrity and meiosis.

View Article: PubMed Central - PubMed

Affiliation: Institute of Plant and Microbial Biology, Academia Sinica, Taipei, 11529, Taiwan.

Show MeSH
Related in: MedlinePlus