Arabidopsis CHROMOSOME TRANSMISSION FIDELITY 7 (AtCTF7/ECO1) is required for DNA repair, mitosis and meiosis.
Bottom Line: No significant change was observed in the expression of genes that influence entry into the endocycle.Analysis of meiocytes identified changes in chromosome morphology and defective segregation; the abundance of chromosomal-bound cohesion subunits was also reduced.Taken together our results demonstrate that Arabidopsis CTF7/ECO1 plays important roles in the preservation of genome integrity and meiosis.
Affiliation: Institute of Plant and Microbial Biology, Academia Sinica, Taipei, 11529, Taiwan.Show MeSH
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Mentions: Given the developmental defects observed in ctf7-1 meiocytes the transcript level of highly expressed meiotic genes (Yang et al., 2011b) was measured by QPCR as well. As shown in Figure 6, statistically significant increases in transcript levels were observed for ATM and ATR, BRCA2B, RAD51C, DMC1, SMC1 and SMC3, and CDC45, a gene that codes for an S-phase licensing factor and is required for meiosis (Stevens et al., 2004). No significant increase was observed for the mitotic checkpoint gene BUB1.3. Expression of several other genes was not detected, including SRS2, mitotic topoisomerases TOPOII-α, TOPOI-α, TOPOI-β (Takahashi et al., 2002), meiotic TOPO III-α (Hartung et al., 2008) or endoreplication factor MYB3R4 (Takahashi et al., 2010).
Affiliation: Institute of Plant and Microbial Biology, Academia Sinica, Taipei, 11529, Taiwan.