Arabidopsis CHROMOSOME TRANSMISSION FIDELITY 7 (AtCTF7/ECO1) is required for DNA repair, mitosis and meiosis.
Bottom Line: No significant change was observed in the expression of genes that influence entry into the endocycle.Analysis of meiocytes identified changes in chromosome morphology and defective segregation; the abundance of chromosomal-bound cohesion subunits was also reduced.Taken together our results demonstrate that Arabidopsis CTF7/ECO1 plays important roles in the preservation of genome integrity and meiosis.
Affiliation: Institute of Plant and Microbial Biology, Academia Sinica, Taipei, 11529, Taiwan.Show MeSH
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Mentions: We next investigated the loading and distribution of the SYN1 and SMC3 cohesin proteins on chromosomes of ctf7-1 meiocytes. As has been demonstrated previously, SYN1 and SMC3 display similar distribution patterns on WT meiotic chromosomes (Yang et al., 2011a,b; Figures 4 and S5 for WT). Diffuse nuclear labeling is observed at interphase. Beginning at early leptotene and extending into zygotene both proteins decorated the developing WT chromosomal axes. During late zygotene and pachytene the proteins lined the synapsed chromosomes. As meiosis progressed from diplotene to diakinesis the chromosome-associated cohesin signals became progressively weaker and more diffuse.
Affiliation: Institute of Plant and Microbial Biology, Academia Sinica, Taipei, 11529, Taiwan.