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Arabidopsis CHROMOSOME TRANSMISSION FIDELITY 7 (AtCTF7/ECO1) is required for DNA repair, mitosis and meiosis.

Bolaños-Villegas P, Yang X, Wang HJ, Juan CT, Chuang MH, Makaroff CA, Jauh GY - Plant J. (2013)

Bottom Line: No significant change was observed in the expression of genes that influence entry into the endocycle.Analysis of meiocytes identified changes in chromosome morphology and defective segregation; the abundance of chromosomal-bound cohesion subunits was also reduced.Taken together our results demonstrate that Arabidopsis CTF7/ECO1 plays important roles in the preservation of genome integrity and meiosis.

View Article: PubMed Central - PubMed

Affiliation: Institute of Plant and Microbial Biology, Academia Sinica, Taipei, 11529, Taiwan.

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Related in: MedlinePlus

SYN1 exhibits an altered distribution pattern in homozygous ctf7-1 male meiocytes. Merged images of 4′,6-diamidino-2-phenylindole stained chromosomes (red) and SYN1 (green) are shown.(a) Interphase. SYN1 is distributed throughout the nuclei of wild type (WT) male meiocytes; in contrast little or no SYN1 was observed within the nuclei of ctf7-1.(b) Leptotene. SYN1 decorated WT chromosome filaments as they started to condense, while in ctf7-1 only labeling is weak and diffuse.(c), (d) Early and late zygotene. SYN1 decorates WT chromosome axes as chromosomes start to synapse.(e) Pachytene. SYN1 lined the synapsed WT bivalents, but in ctf7-1 the labeling remained weak and diffuse.(f) Diplotene. SYN1 continues to label WT bivalents, while in ctf7-1 both proteins appear as scattered, punctuate foci.(g) Diakinesis. Chromosome-associated SYN1 begins to weaken in WT and is absent in ctf7-1 nuclei. Scale bars = 10 μm.
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fig04: SYN1 exhibits an altered distribution pattern in homozygous ctf7-1 male meiocytes. Merged images of 4′,6-diamidino-2-phenylindole stained chromosomes (red) and SYN1 (green) are shown.(a) Interphase. SYN1 is distributed throughout the nuclei of wild type (WT) male meiocytes; in contrast little or no SYN1 was observed within the nuclei of ctf7-1.(b) Leptotene. SYN1 decorated WT chromosome filaments as they started to condense, while in ctf7-1 only labeling is weak and diffuse.(c), (d) Early and late zygotene. SYN1 decorates WT chromosome axes as chromosomes start to synapse.(e) Pachytene. SYN1 lined the synapsed WT bivalents, but in ctf7-1 the labeling remained weak and diffuse.(f) Diplotene. SYN1 continues to label WT bivalents, while in ctf7-1 both proteins appear as scattered, punctuate foci.(g) Diakinesis. Chromosome-associated SYN1 begins to weaken in WT and is absent in ctf7-1 nuclei. Scale bars = 10 μm.

Mentions: We next investigated the loading and distribution of the SYN1 and SMC3 cohesin proteins on chromosomes of ctf7-1 meiocytes. As has been demonstrated previously, SYN1 and SMC3 display similar distribution patterns on WT meiotic chromosomes (Yang et al., 2011a,b; Figures 4 and S5 for WT). Diffuse nuclear labeling is observed at interphase. Beginning at early leptotene and extending into zygotene both proteins decorated the developing WT chromosomal axes. During late zygotene and pachytene the proteins lined the synapsed chromosomes. As meiosis progressed from diplotene to diakinesis the chromosome-associated cohesin signals became progressively weaker and more diffuse.


Arabidopsis CHROMOSOME TRANSMISSION FIDELITY 7 (AtCTF7/ECO1) is required for DNA repair, mitosis and meiosis.

Bolaños-Villegas P, Yang X, Wang HJ, Juan CT, Chuang MH, Makaroff CA, Jauh GY - Plant J. (2013)

SYN1 exhibits an altered distribution pattern in homozygous ctf7-1 male meiocytes. Merged images of 4′,6-diamidino-2-phenylindole stained chromosomes (red) and SYN1 (green) are shown.(a) Interphase. SYN1 is distributed throughout the nuclei of wild type (WT) male meiocytes; in contrast little or no SYN1 was observed within the nuclei of ctf7-1.(b) Leptotene. SYN1 decorated WT chromosome filaments as they started to condense, while in ctf7-1 only labeling is weak and diffuse.(c), (d) Early and late zygotene. SYN1 decorates WT chromosome axes as chromosomes start to synapse.(e) Pachytene. SYN1 lined the synapsed WT bivalents, but in ctf7-1 the labeling remained weak and diffuse.(f) Diplotene. SYN1 continues to label WT bivalents, while in ctf7-1 both proteins appear as scattered, punctuate foci.(g) Diakinesis. Chromosome-associated SYN1 begins to weaken in WT and is absent in ctf7-1 nuclei. Scale bars = 10 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3824207&req=5

fig04: SYN1 exhibits an altered distribution pattern in homozygous ctf7-1 male meiocytes. Merged images of 4′,6-diamidino-2-phenylindole stained chromosomes (red) and SYN1 (green) are shown.(a) Interphase. SYN1 is distributed throughout the nuclei of wild type (WT) male meiocytes; in contrast little or no SYN1 was observed within the nuclei of ctf7-1.(b) Leptotene. SYN1 decorated WT chromosome filaments as they started to condense, while in ctf7-1 only labeling is weak and diffuse.(c), (d) Early and late zygotene. SYN1 decorates WT chromosome axes as chromosomes start to synapse.(e) Pachytene. SYN1 lined the synapsed WT bivalents, but in ctf7-1 the labeling remained weak and diffuse.(f) Diplotene. SYN1 continues to label WT bivalents, while in ctf7-1 both proteins appear as scattered, punctuate foci.(g) Diakinesis. Chromosome-associated SYN1 begins to weaken in WT and is absent in ctf7-1 nuclei. Scale bars = 10 μm.
Mentions: We next investigated the loading and distribution of the SYN1 and SMC3 cohesin proteins on chromosomes of ctf7-1 meiocytes. As has been demonstrated previously, SYN1 and SMC3 display similar distribution patterns on WT meiotic chromosomes (Yang et al., 2011a,b; Figures 4 and S5 for WT). Diffuse nuclear labeling is observed at interphase. Beginning at early leptotene and extending into zygotene both proteins decorated the developing WT chromosomal axes. During late zygotene and pachytene the proteins lined the synapsed chromosomes. As meiosis progressed from diplotene to diakinesis the chromosome-associated cohesin signals became progressively weaker and more diffuse.

Bottom Line: No significant change was observed in the expression of genes that influence entry into the endocycle.Analysis of meiocytes identified changes in chromosome morphology and defective segregation; the abundance of chromosomal-bound cohesion subunits was also reduced.Taken together our results demonstrate that Arabidopsis CTF7/ECO1 plays important roles in the preservation of genome integrity and meiosis.

View Article: PubMed Central - PubMed

Affiliation: Institute of Plant and Microbial Biology, Academia Sinica, Taipei, 11529, Taiwan.

Show MeSH
Related in: MedlinePlus