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Multi-scale spatial heterogeneity of pectic rhamnogalacturonan I (RG-I) structural features in tobacco seed endosperm cell walls.

Lee KJ, Cornuault V, Manfield IW, Ralet MC, Knox JP - Plant J. (2013)

Bottom Line: Heterogeneous RG-I polymers are implicated in generating the mechanical properties of cell walls during cell development and plant growth, but are poorly understood in architectural, biochemical and functional terms.The analyses indicate that the features of the RG-I polymer display spatial heterogeneity at the level of the tissue and the level of single cell walls, and also heterogeneity at the biochemical level.This work has implications for understanding RG-I glycan complexity in the context of cell-wall architectures and in relation to cell-wall functions in cell and tissue development.

View Article: PubMed Central - PubMed

Affiliation: Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK.

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Quantification of immunofluorescence signals in transects across cell walls in adhered regions of cells in the NME of tobacco seeds. The profiles show relative fluorescence for LM5 galactan, LM6 arabinan and RU1 RG backbone epitopes in equivalent sections that had received no pre-treatment (black lines) or a pre-treatment with endo-β–mannanase (grey lines, +). Fluorescence profiles are the means of three equivalent transects taken from the regions indicated by yellow stars in Figure 2 and yellow lines in Figure 3.
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fig04: Quantification of immunofluorescence signals in transects across cell walls in adhered regions of cells in the NME of tobacco seeds. The profiles show relative fluorescence for LM5 galactan, LM6 arabinan and RU1 RG backbone epitopes in equivalent sections that had received no pre-treatment (black lines) or a pre-treatment with endo-β–mannanase (grey lines, +). Fluorescence profiles are the means of three equivalent transects taken from the regions indicated by yellow stars in Figure 2 and yellow lines in Figure 3.

Mentions: Indirect immunofluorescence labelling of sections through tobacco endosperm cells immuno with LM5 galactan, LM6 arabinan and RU1 RG backbone antibodies using untreated sections (left) and after pre-treatment with endo-β–mannanase (+EβM) to remove the abundant heteromannan (right). The yellow lines indicate cell-wall regions where transects of fluorescence intensity were determined (see Figure 4). Scale bar = 10 μm.


Multi-scale spatial heterogeneity of pectic rhamnogalacturonan I (RG-I) structural features in tobacco seed endosperm cell walls.

Lee KJ, Cornuault V, Manfield IW, Ralet MC, Knox JP - Plant J. (2013)

Quantification of immunofluorescence signals in transects across cell walls in adhered regions of cells in the NME of tobacco seeds. The profiles show relative fluorescence for LM5 galactan, LM6 arabinan and RU1 RG backbone epitopes in equivalent sections that had received no pre-treatment (black lines) or a pre-treatment with endo-β–mannanase (grey lines, +). Fluorescence profiles are the means of three equivalent transects taken from the regions indicated by yellow stars in Figure 2 and yellow lines in Figure 3.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3824205&req=5

fig04: Quantification of immunofluorescence signals in transects across cell walls in adhered regions of cells in the NME of tobacco seeds. The profiles show relative fluorescence for LM5 galactan, LM6 arabinan and RU1 RG backbone epitopes in equivalent sections that had received no pre-treatment (black lines) or a pre-treatment with endo-β–mannanase (grey lines, +). Fluorescence profiles are the means of three equivalent transects taken from the regions indicated by yellow stars in Figure 2 and yellow lines in Figure 3.
Mentions: Indirect immunofluorescence labelling of sections through tobacco endosperm cells immuno with LM5 galactan, LM6 arabinan and RU1 RG backbone antibodies using untreated sections (left) and after pre-treatment with endo-β–mannanase (+EβM) to remove the abundant heteromannan (right). The yellow lines indicate cell-wall regions where transects of fluorescence intensity were determined (see Figure 4). Scale bar = 10 μm.

Bottom Line: Heterogeneous RG-I polymers are implicated in generating the mechanical properties of cell walls during cell development and plant growth, but are poorly understood in architectural, biochemical and functional terms.The analyses indicate that the features of the RG-I polymer display spatial heterogeneity at the level of the tissue and the level of single cell walls, and also heterogeneity at the biochemical level.This work has implications for understanding RG-I glycan complexity in the context of cell-wall architectures and in relation to cell-wall functions in cell and tissue development.

View Article: PubMed Central - PubMed

Affiliation: Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK.

Show MeSH
Related in: MedlinePlus