Multi-scale spatial heterogeneity of pectic rhamnogalacturonan I (RG-I) structural features in tobacco seed endosperm cell walls.
Bottom Line: Heterogeneous RG-I polymers are implicated in generating the mechanical properties of cell walls during cell development and plant growth, but are poorly understood in architectural, biochemical and functional terms.The analyses indicate that the features of the RG-I polymer display spatial heterogeneity at the level of the tissue and the level of single cell walls, and also heterogeneity at the biochemical level.This work has implications for understanding RG-I glycan complexity in the context of cell-wall architectures and in relation to cell-wall functions in cell and tissue development.
Affiliation: Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK.Show MeSH
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Mentions: Indirect immunofluorescence labelling of sections through tobacco endosperm cells immuno with LM5 galactan, LM6 arabinan and RU1 RG backbone antibodies using untreated sections (left) and after pre-treatment with endo-β–mannanase (+EβM) to remove the abundant heteromannan (right). The yellow lines indicate cell-wall regions where transects of fluorescence intensity were determined (see Figure 4). Scale bar = 10 μm.
Affiliation: Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK.