Multi-scale spatial heterogeneity of pectic rhamnogalacturonan I (RG-I) structural features in tobacco seed endosperm cell walls.
Bottom Line: Heterogeneous RG-I polymers are implicated in generating the mechanical properties of cell walls during cell development and plant growth, but are poorly understood in architectural, biochemical and functional terms.The analyses indicate that the features of the RG-I polymer display spatial heterogeneity at the level of the tissue and the level of single cell walls, and also heterogeneity at the biochemical level.This work has implications for understanding RG-I glycan complexity in the context of cell-wall architectures and in relation to cell-wall functions in cell and tissue development.
Affiliation: Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK.Show MeSH
Mentions: Indirect immunofluorescence labelling of sections through tobacco seeds with LM5 galactan and RU1 rhamnogalacturonan I backbone probes. In the lower micrographs of the micrograph pairs, equivalent sections were treated with with endo-β–mannanase (+EβM) to remove the abundant heteromannan. White dashed lines with an asterisk indicate that a tissue asymmetry was detected. Brown dashed lines indicate no tissue level asymmetry was detected. The yellow stars indicate the region of peripheral endosperm used for fluorescence imaging at higher magnification (see Figure 3). Scale bar = 50 μm.
Affiliation: Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK.