Multi-scale spatial heterogeneity of pectic rhamnogalacturonan I (RG-I) structural features in tobacco seed endosperm cell walls.
Bottom Line: Heterogeneous RG-I polymers are implicated in generating the mechanical properties of cell walls during cell development and plant growth, but are poorly understood in architectural, biochemical and functional terms.The analyses indicate that the features of the RG-I polymer display spatial heterogeneity at the level of the tissue and the level of single cell walls, and also heterogeneity at the biochemical level.This work has implications for understanding RG-I glycan complexity in the context of cell-wall architectures and in relation to cell-wall functions in cell and tissue development.
Affiliation: Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK.Show MeSH
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Mentions: Immunolabelling of equivalent sections of tobacco seeds with the LM5 galactan monoclonal antibody revealed tissue asymmetry, in that the epitope was detected in the NME but not in the ME region (Figure 2). After enzymatic removal of heteromannan, detection of the LM5 galactan increased in terms of fluorescence intensity, but there was no change in the asymmetry of its occurrence as shown in Figure 2. Further enzymatic deconstructions of endosperm cell walls involving enzymatic removal of pectic HG and pectic arabinan, which are known to be abundant in the ME cell walls (Lee et al., 2012), did not result in increased detection of the LM5 galactan epitope in this region.
Affiliation: Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK.