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Geraniol ameliorates the motor behavior and neurotrophic factors inadequacy in MPTP-induced mice model of Parkinson's disease.

Rekha KR, Selvakumar GP, Sethupathy S, Santha K, Sivakamasundari RI - J. Mol. Neurosci. (2013)

Bottom Line: Many experiments affirm the notion that augmentation of neurotrophic factors (NTFs) activity, especially brain-derived neurotrophic factors and glial cell-derived neurotrophic factors, could prevent or halt the progress of neurodegeneration in Parkinson's disease (PD).Systematically, the loss of dopaminergic neurons and reduced NTFs mRNA expressions induced by MPTP was ameliorated to a significant extent by pretreatment with GE.We found that GE confers a potent neuroprotective agent against MPTP-induced dopaminergic denervation and may become a potential therapeutic agent for PD and/or its progression.

View Article: PubMed Central - PubMed

Affiliation: Division of Biochemistry, Faculty of Medicine, Raja Muthaiah Medical College, Annamalai University, 608 002, Annamalainagar, Tamil Nadu, India.

ABSTRACT
Many experiments affirm the notion that augmentation of neurotrophic factors (NTFs) activity, especially brain-derived neurotrophic factors and glial cell-derived neurotrophic factors, could prevent or halt the progress of neurodegeneration in Parkinson's disease (PD). In this study, we investigated the therapeutic accomplishment of geraniol (GE 100 mg/kg) on 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP)-induced mice model of PD. Current investigation proved that pretreatment with GE ameliorates the MPTP-induced alterations in behavioral, biochemical, immunohistochemical, and immunoblotting manifestations in mice. Systematically, the loss of dopaminergic neurons and reduced NTFs mRNA expressions induced by MPTP was ameliorated to a significant extent by pretreatment with GE. We found that GE confers a potent neuroprotective agent against MPTP-induced dopaminergic denervation and may become a potential therapeutic agent for PD and/or its progression.

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Related in: MedlinePlus

a Effect of GE on TH expression in SN. Representative microphotographs of the expression of TH was almost negligible in MPTP group as compared to control group, while the MPTP group treated with GE has shown a moderate staining of TH. However, the GE control group has shown no discernible change in TH staining as compared to control group. Original magnifications at ×4 and ×40. b Quantification of TH-IR was performed by counting the number of TH-IR neurons in SN. The mean value for TH-IR was determined for each group and was expressed as a percentage of that matched control mice. Values are expressed as mean ± SD of three mice per group. aP < 0.05, compared with the control. bP < 0.05, compared with the MPTP control group
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Fig4: a Effect of GE on TH expression in SN. Representative microphotographs of the expression of TH was almost negligible in MPTP group as compared to control group, while the MPTP group treated with GE has shown a moderate staining of TH. However, the GE control group has shown no discernible change in TH staining as compared to control group. Original magnifications at ×4 and ×40. b Quantification of TH-IR was performed by counting the number of TH-IR neurons in SN. The mean value for TH-IR was determined for each group and was expressed as a percentage of that matched control mice. Values are expressed as mean ± SD of three mice per group. aP < 0.05, compared with the control. bP < 0.05, compared with the MPTP control group

Mentions: Representative microphotographs of TH immunostaining in the SN were shown in Fig. 4a, b. Animals that received the treatment of MPTP injection showed a marked loss of TH-immunopositive neurons in the SN compared with the control group. In contrast, GE-treated mice showed significantly reduced nigrostriatal dopaminergic neuron loss following MPTP injection at the dose of 30 mg/kg as compared to MPTP alone treated group (P < 0.05).Fig. 4


Geraniol ameliorates the motor behavior and neurotrophic factors inadequacy in MPTP-induced mice model of Parkinson's disease.

Rekha KR, Selvakumar GP, Sethupathy S, Santha K, Sivakamasundari RI - J. Mol. Neurosci. (2013)

a Effect of GE on TH expression in SN. Representative microphotographs of the expression of TH was almost negligible in MPTP group as compared to control group, while the MPTP group treated with GE has shown a moderate staining of TH. However, the GE control group has shown no discernible change in TH staining as compared to control group. Original magnifications at ×4 and ×40. b Quantification of TH-IR was performed by counting the number of TH-IR neurons in SN. The mean value for TH-IR was determined for each group and was expressed as a percentage of that matched control mice. Values are expressed as mean ± SD of three mice per group. aP < 0.05, compared with the control. bP < 0.05, compared with the MPTP control group
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3824202&req=5

Fig4: a Effect of GE on TH expression in SN. Representative microphotographs of the expression of TH was almost negligible in MPTP group as compared to control group, while the MPTP group treated with GE has shown a moderate staining of TH. However, the GE control group has shown no discernible change in TH staining as compared to control group. Original magnifications at ×4 and ×40. b Quantification of TH-IR was performed by counting the number of TH-IR neurons in SN. The mean value for TH-IR was determined for each group and was expressed as a percentage of that matched control mice. Values are expressed as mean ± SD of three mice per group. aP < 0.05, compared with the control. bP < 0.05, compared with the MPTP control group
Mentions: Representative microphotographs of TH immunostaining in the SN were shown in Fig. 4a, b. Animals that received the treatment of MPTP injection showed a marked loss of TH-immunopositive neurons in the SN compared with the control group. In contrast, GE-treated mice showed significantly reduced nigrostriatal dopaminergic neuron loss following MPTP injection at the dose of 30 mg/kg as compared to MPTP alone treated group (P < 0.05).Fig. 4

Bottom Line: Many experiments affirm the notion that augmentation of neurotrophic factors (NTFs) activity, especially brain-derived neurotrophic factors and glial cell-derived neurotrophic factors, could prevent or halt the progress of neurodegeneration in Parkinson's disease (PD).Systematically, the loss of dopaminergic neurons and reduced NTFs mRNA expressions induced by MPTP was ameliorated to a significant extent by pretreatment with GE.We found that GE confers a potent neuroprotective agent against MPTP-induced dopaminergic denervation and may become a potential therapeutic agent for PD and/or its progression.

View Article: PubMed Central - PubMed

Affiliation: Division of Biochemistry, Faculty of Medicine, Raja Muthaiah Medical College, Annamalai University, 608 002, Annamalainagar, Tamil Nadu, India.

ABSTRACT
Many experiments affirm the notion that augmentation of neurotrophic factors (NTFs) activity, especially brain-derived neurotrophic factors and glial cell-derived neurotrophic factors, could prevent or halt the progress of neurodegeneration in Parkinson's disease (PD). In this study, we investigated the therapeutic accomplishment of geraniol (GE 100 mg/kg) on 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP)-induced mice model of PD. Current investigation proved that pretreatment with GE ameliorates the MPTP-induced alterations in behavioral, biochemical, immunohistochemical, and immunoblotting manifestations in mice. Systematically, the loss of dopaminergic neurons and reduced NTFs mRNA expressions induced by MPTP was ameliorated to a significant extent by pretreatment with GE. We found that GE confers a potent neuroprotective agent against MPTP-induced dopaminergic denervation and may become a potential therapeutic agent for PD and/or its progression.

Show MeSH
Related in: MedlinePlus