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Elevated ground-level O3 negatively influences paddy methanogenic archaeal community.

Feng Y, Lin X, Yu Y, Zhang H, Chu H, Zhu J - Sci Rep (2013)

Bottom Line: The current knowledge regarding the effect of global climate change on rice-paddy methane (CH4) emissions is incomplete, partly because information is limited concerning the mechanism of the microbial response to elevated ground-level ozone (O3).We found that elevated ground-level O3 inhibited methanogenic activity and influenced the composition of paddy methanogenic communities, reducing the abundance and diversity of paddy methanogens by adversely affecting dominant groups, such as aceticlastic Methanosaeta, especially at the rice tillering stage.Our results indicated that continuously elevated ground-level O3 would negatively influence paddy methanogenic archaeal communities and its critical ecological function.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences Nanjing, 210008, Jiangsu Province P.R. China.

ABSTRACT
The current knowledge regarding the effect of global climate change on rice-paddy methane (CH4) emissions is incomplete, partly because information is limited concerning the mechanism of the microbial response to elevated ground-level ozone (O3). A field experiment was conducted in the China Ozone Free-Air Concentration Enrichment facility in a rice-wheat rotation system to investigate the responses of methanogenic archaeal communities to elevated ground-level O3 by culture-independent and -reliant approaches. We found that elevated ground-level O3 inhibited methanogenic activity and influenced the composition of paddy methanogenic communities, reducing the abundance and diversity of paddy methanogens by adversely affecting dominant groups, such as aceticlastic Methanosaeta, especially at the rice tillering stage. Our results indicated that continuously elevated ground-level O3 would negatively influence paddy methanogenic archaeal communities and its critical ecological function. These findings will contribute to a comprehensive understanding of the responses and feedbacks of paddy ecosystems to global climate change.

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Significant changes in genera under elevated ground-level O3 according to the response ratio method at a 95% confidence interval.
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f5: Significant changes in genera under elevated ground-level O3 according to the response ratio method at a 95% confidence interval.

Mentions: Response ratios were calculated based on the sequence size of each genus (Fig. 5). The 95% confidence interval (CI) at the rice tillering stage ranged from −0.01 to −0.17 and did not overlap with 0, which indicates that elevated ground-level O3 brings a significant negative influence on the methanogenic archaeal community composition at the rice tillering stage (p < 0.05). Compared to ambient O3, a total of 12 genera were significantly (p < 0.05) decreased and 6 genera were significantly (p < 0.05) increased under elevated ground-level O3 at the rice tillering stage. The dominant genera, namely Methanosaeta, Methanosarcina and Methanocella, significantly decreased under elevated ground-level O3 (p < 0.05). At the rice anthesis stage, the 95% CI, ranging from 0.19 to 0.05, revealed a significant positive effect of elevated ground-level O3 on methanogenic archaeal community composition (p < 0.05): under elevated ground-level O3, 12 genera were significantly increased and 7 genera were significantly decreased (p < 0.05). For example, Methanosarcina, Methanocella and Methanocellaceae clones isolated from paddy soil were significantly increased by elevated ground-level O3 (p < 0.05). The results of the Venn diagram and response ratios are consistent with the general trends of the taxonomic distributions of paddy methanogenic archaea (Fig. 2), PD index, Chao1 index (Table 1) and NMDS plot (Fig. 3). In summary, elevated ground-level O3 influenced the phylogenetic composition of paddy methanogenic archaeal community and significantly decreased their diversity at the rice tillering stage (p < 0.05).


Elevated ground-level O3 negatively influences paddy methanogenic archaeal community.

Feng Y, Lin X, Yu Y, Zhang H, Chu H, Zhu J - Sci Rep (2013)

Significant changes in genera under elevated ground-level O3 according to the response ratio method at a 95% confidence interval.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3824163&req=5

f5: Significant changes in genera under elevated ground-level O3 according to the response ratio method at a 95% confidence interval.
Mentions: Response ratios were calculated based on the sequence size of each genus (Fig. 5). The 95% confidence interval (CI) at the rice tillering stage ranged from −0.01 to −0.17 and did not overlap with 0, which indicates that elevated ground-level O3 brings a significant negative influence on the methanogenic archaeal community composition at the rice tillering stage (p < 0.05). Compared to ambient O3, a total of 12 genera were significantly (p < 0.05) decreased and 6 genera were significantly (p < 0.05) increased under elevated ground-level O3 at the rice tillering stage. The dominant genera, namely Methanosaeta, Methanosarcina and Methanocella, significantly decreased under elevated ground-level O3 (p < 0.05). At the rice anthesis stage, the 95% CI, ranging from 0.19 to 0.05, revealed a significant positive effect of elevated ground-level O3 on methanogenic archaeal community composition (p < 0.05): under elevated ground-level O3, 12 genera were significantly increased and 7 genera were significantly decreased (p < 0.05). For example, Methanosarcina, Methanocella and Methanocellaceae clones isolated from paddy soil were significantly increased by elevated ground-level O3 (p < 0.05). The results of the Venn diagram and response ratios are consistent with the general trends of the taxonomic distributions of paddy methanogenic archaea (Fig. 2), PD index, Chao1 index (Table 1) and NMDS plot (Fig. 3). In summary, elevated ground-level O3 influenced the phylogenetic composition of paddy methanogenic archaeal community and significantly decreased their diversity at the rice tillering stage (p < 0.05).

Bottom Line: The current knowledge regarding the effect of global climate change on rice-paddy methane (CH4) emissions is incomplete, partly because information is limited concerning the mechanism of the microbial response to elevated ground-level ozone (O3).We found that elevated ground-level O3 inhibited methanogenic activity and influenced the composition of paddy methanogenic communities, reducing the abundance and diversity of paddy methanogens by adversely affecting dominant groups, such as aceticlastic Methanosaeta, especially at the rice tillering stage.Our results indicated that continuously elevated ground-level O3 would negatively influence paddy methanogenic archaeal communities and its critical ecological function.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences Nanjing, 210008, Jiangsu Province P.R. China.

ABSTRACT
The current knowledge regarding the effect of global climate change on rice-paddy methane (CH4) emissions is incomplete, partly because information is limited concerning the mechanism of the microbial response to elevated ground-level ozone (O3). A field experiment was conducted in the China Ozone Free-Air Concentration Enrichment facility in a rice-wheat rotation system to investigate the responses of methanogenic archaeal communities to elevated ground-level O3 by culture-independent and -reliant approaches. We found that elevated ground-level O3 inhibited methanogenic activity and influenced the composition of paddy methanogenic communities, reducing the abundance and diversity of paddy methanogens by adversely affecting dominant groups, such as aceticlastic Methanosaeta, especially at the rice tillering stage. Our results indicated that continuously elevated ground-level O3 would negatively influence paddy methanogenic archaeal communities and its critical ecological function. These findings will contribute to a comprehensive understanding of the responses and feedbacks of paddy ecosystems to global climate change.

Show MeSH