Limits...
Elevated ground-level O3 negatively influences paddy methanogenic archaeal community.

Feng Y, Lin X, Yu Y, Zhang H, Chu H, Zhu J - Sci Rep (2013)

Bottom Line: The current knowledge regarding the effect of global climate change on rice-paddy methane (CH4) emissions is incomplete, partly because information is limited concerning the mechanism of the microbial response to elevated ground-level ozone (O3).We found that elevated ground-level O3 inhibited methanogenic activity and influenced the composition of paddy methanogenic communities, reducing the abundance and diversity of paddy methanogens by adversely affecting dominant groups, such as aceticlastic Methanosaeta, especially at the rice tillering stage.Our results indicated that continuously elevated ground-level O3 would negatively influence paddy methanogenic archaeal communities and its critical ecological function.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences Nanjing, 210008, Jiangsu Province P.R. China.

ABSTRACT
The current knowledge regarding the effect of global climate change on rice-paddy methane (CH4) emissions is incomplete, partly because information is limited concerning the mechanism of the microbial response to elevated ground-level ozone (O3). A field experiment was conducted in the China Ozone Free-Air Concentration Enrichment facility in a rice-wheat rotation system to investigate the responses of methanogenic archaeal communities to elevated ground-level O3 by culture-independent and -reliant approaches. We found that elevated ground-level O3 inhibited methanogenic activity and influenced the composition of paddy methanogenic communities, reducing the abundance and diversity of paddy methanogens by adversely affecting dominant groups, such as aceticlastic Methanosaeta, especially at the rice tillering stage. Our results indicated that continuously elevated ground-level O3 would negatively influence paddy methanogenic archaeal communities and its critical ecological function. These findings will contribute to a comprehensive understanding of the responses and feedbacks of paddy ecosystems to global climate change.

Show MeSH
The 100% stacked column chart of relative abundances of the dominant methanogenic genera derived from 16 s rRNA genes in all paddy soils combined and in each soil sample.*The value of each genus percentage is the mean of triplicates in Table S1.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3824163&req=5

f2: The 100% stacked column chart of relative abundances of the dominant methanogenic genera derived from 16 s rRNA genes in all paddy soils combined and in each soil sample.*The value of each genus percentage is the mean of triplicates in Table S1.

Mentions: A total of 87,688 sequences were obtained (Table S1). Of these sequences, 91.3% were affiliated with methanogenic archaea. Pyrosequencing revealed that the paddy methanogenic archaeal community was dominated by two classes, namely Methanomicrobia (81.1%) and Methanobacteria (10.2%). With higher resolution, we found that Methanosarcinales were most abundant (50.5%), followed by Methanomicrobiales (17.6%) and Methanocellales (13.0%), at the order level. At the family level, the dominant methanogenic archaea were found to be aceticlastic groups, including Methanosaetaceae (32.0%) and Methanosarcinaceae (14.1%), followed by Methanocellaceae (Rice cluster I) (13.0%) and Methanobacteriaceae (10.2%). At the genus level, the dominant methanogenic groups were Methanosaeta (32.0%), Methanosarcina (14.0%), Methanobacterium (10.1%) and Methanocella (7.0%) (Fig. 2).


Elevated ground-level O3 negatively influences paddy methanogenic archaeal community.

Feng Y, Lin X, Yu Y, Zhang H, Chu H, Zhu J - Sci Rep (2013)

The 100% stacked column chart of relative abundances of the dominant methanogenic genera derived from 16 s rRNA genes in all paddy soils combined and in each soil sample.*The value of each genus percentage is the mean of triplicates in Table S1.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3824163&req=5

f2: The 100% stacked column chart of relative abundances of the dominant methanogenic genera derived from 16 s rRNA genes in all paddy soils combined and in each soil sample.*The value of each genus percentage is the mean of triplicates in Table S1.
Mentions: A total of 87,688 sequences were obtained (Table S1). Of these sequences, 91.3% were affiliated with methanogenic archaea. Pyrosequencing revealed that the paddy methanogenic archaeal community was dominated by two classes, namely Methanomicrobia (81.1%) and Methanobacteria (10.2%). With higher resolution, we found that Methanosarcinales were most abundant (50.5%), followed by Methanomicrobiales (17.6%) and Methanocellales (13.0%), at the order level. At the family level, the dominant methanogenic archaea were found to be aceticlastic groups, including Methanosaetaceae (32.0%) and Methanosarcinaceae (14.1%), followed by Methanocellaceae (Rice cluster I) (13.0%) and Methanobacteriaceae (10.2%). At the genus level, the dominant methanogenic groups were Methanosaeta (32.0%), Methanosarcina (14.0%), Methanobacterium (10.1%) and Methanocella (7.0%) (Fig. 2).

Bottom Line: The current knowledge regarding the effect of global climate change on rice-paddy methane (CH4) emissions is incomplete, partly because information is limited concerning the mechanism of the microbial response to elevated ground-level ozone (O3).We found that elevated ground-level O3 inhibited methanogenic activity and influenced the composition of paddy methanogenic communities, reducing the abundance and diversity of paddy methanogens by adversely affecting dominant groups, such as aceticlastic Methanosaeta, especially at the rice tillering stage.Our results indicated that continuously elevated ground-level O3 would negatively influence paddy methanogenic archaeal communities and its critical ecological function.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences Nanjing, 210008, Jiangsu Province P.R. China.

ABSTRACT
The current knowledge regarding the effect of global climate change on rice-paddy methane (CH4) emissions is incomplete, partly because information is limited concerning the mechanism of the microbial response to elevated ground-level ozone (O3). A field experiment was conducted in the China Ozone Free-Air Concentration Enrichment facility in a rice-wheat rotation system to investigate the responses of methanogenic archaeal communities to elevated ground-level O3 by culture-independent and -reliant approaches. We found that elevated ground-level O3 inhibited methanogenic activity and influenced the composition of paddy methanogenic communities, reducing the abundance and diversity of paddy methanogens by adversely affecting dominant groups, such as aceticlastic Methanosaeta, especially at the rice tillering stage. Our results indicated that continuously elevated ground-level O3 would negatively influence paddy methanogenic archaeal communities and its critical ecological function. These findings will contribute to a comprehensive understanding of the responses and feedbacks of paddy ecosystems to global climate change.

Show MeSH