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Elevated ground-level O3 negatively influences paddy methanogenic archaeal community.

Feng Y, Lin X, Yu Y, Zhang H, Chu H, Zhu J - Sci Rep (2013)

Bottom Line: The current knowledge regarding the effect of global climate change on rice-paddy methane (CH4) emissions is incomplete, partly because information is limited concerning the mechanism of the microbial response to elevated ground-level ozone (O3).We found that elevated ground-level O3 inhibited methanogenic activity and influenced the composition of paddy methanogenic communities, reducing the abundance and diversity of paddy methanogens by adversely affecting dominant groups, such as aceticlastic Methanosaeta, especially at the rice tillering stage.Our results indicated that continuously elevated ground-level O3 would negatively influence paddy methanogenic archaeal communities and its critical ecological function.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences Nanjing, 210008, Jiangsu Province P.R. China.

ABSTRACT
The current knowledge regarding the effect of global climate change on rice-paddy methane (CH4) emissions is incomplete, partly because information is limited concerning the mechanism of the microbial response to elevated ground-level ozone (O3). A field experiment was conducted in the China Ozone Free-Air Concentration Enrichment facility in a rice-wheat rotation system to investigate the responses of methanogenic archaeal communities to elevated ground-level O3 by culture-independent and -reliant approaches. We found that elevated ground-level O3 inhibited methanogenic activity and influenced the composition of paddy methanogenic communities, reducing the abundance and diversity of paddy methanogens by adversely affecting dominant groups, such as aceticlastic Methanosaeta, especially at the rice tillering stage. Our results indicated that continuously elevated ground-level O3 would negatively influence paddy methanogenic archaeal communities and its critical ecological function. These findings will contribute to a comprehensive understanding of the responses and feedbacks of paddy ecosystems to global climate change.

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Related in: MedlinePlus

Concentrations of dissolved organic C (DOC) and acetate in paddy soils (a), copy numbers of methanogenic archaeal and Methanosaeta 16 s rRNA genes (b) and the dynamic curves of methane production in incubated flooded paddy soils (c) under elevated ground-level O3 (FACE-O3) and ambient O3 (Ambient) concentrations.Data are expressed as the means with standard deviation (SD). There were 12 soil samples in total. Significant differences are indicated by different letters shown above the error bars (p < 0.05), and mean separation was assessed by Tukey's multiple range test. The capital, small and underlined letters indicate different assays.
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f1: Concentrations of dissolved organic C (DOC) and acetate in paddy soils (a), copy numbers of methanogenic archaeal and Methanosaeta 16 s rRNA genes (b) and the dynamic curves of methane production in incubated flooded paddy soils (c) under elevated ground-level O3 (FACE-O3) and ambient O3 (Ambient) concentrations.Data are expressed as the means with standard deviation (SD). There were 12 soil samples in total. Significant differences are indicated by different letters shown above the error bars (p < 0.05), and mean separation was assessed by Tukey's multiple range test. The capital, small and underlined letters indicate different assays.

Mentions: During rice growth, soil dissolved organic C (DOC) concentrations ranged from 80 ± 6.6 to 103 ± 6.1 mg/kg dry weight soil (d.w.s) (Fig. 1a). According to a pairwise comparison, elevated ground-level O3 significantly decreased the soil DOC concentration under ambient O3 (p < 0.05), from 100 ± 3.6–103 ± 6.1 to 80 ± 6.6–90 ± 4.0 mg/kg d.w.s. The acetate content was measured because it is one of the most abundant low-molecular-weight organic acids in paddy soil and one of the main precursors of CH4 production. As shown in Fig. 1a, elevated ground-level O3 significantly decreased the acetate contents under ambient O3, regardless of the rice growth stage (p < 0.05), from 1.80 ± 0.17–2.12 ± 0.20 to 1.34 ± 0.26–1.49 ± 0.13 mM.


Elevated ground-level O3 negatively influences paddy methanogenic archaeal community.

Feng Y, Lin X, Yu Y, Zhang H, Chu H, Zhu J - Sci Rep (2013)

Concentrations of dissolved organic C (DOC) and acetate in paddy soils (a), copy numbers of methanogenic archaeal and Methanosaeta 16 s rRNA genes (b) and the dynamic curves of methane production in incubated flooded paddy soils (c) under elevated ground-level O3 (FACE-O3) and ambient O3 (Ambient) concentrations.Data are expressed as the means with standard deviation (SD). There were 12 soil samples in total. Significant differences are indicated by different letters shown above the error bars (p < 0.05), and mean separation was assessed by Tukey's multiple range test. The capital, small and underlined letters indicate different assays.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3824163&req=5

f1: Concentrations of dissolved organic C (DOC) and acetate in paddy soils (a), copy numbers of methanogenic archaeal and Methanosaeta 16 s rRNA genes (b) and the dynamic curves of methane production in incubated flooded paddy soils (c) under elevated ground-level O3 (FACE-O3) and ambient O3 (Ambient) concentrations.Data are expressed as the means with standard deviation (SD). There were 12 soil samples in total. Significant differences are indicated by different letters shown above the error bars (p < 0.05), and mean separation was assessed by Tukey's multiple range test. The capital, small and underlined letters indicate different assays.
Mentions: During rice growth, soil dissolved organic C (DOC) concentrations ranged from 80 ± 6.6 to 103 ± 6.1 mg/kg dry weight soil (d.w.s) (Fig. 1a). According to a pairwise comparison, elevated ground-level O3 significantly decreased the soil DOC concentration under ambient O3 (p < 0.05), from 100 ± 3.6–103 ± 6.1 to 80 ± 6.6–90 ± 4.0 mg/kg d.w.s. The acetate content was measured because it is one of the most abundant low-molecular-weight organic acids in paddy soil and one of the main precursors of CH4 production. As shown in Fig. 1a, elevated ground-level O3 significantly decreased the acetate contents under ambient O3, regardless of the rice growth stage (p < 0.05), from 1.80 ± 0.17–2.12 ± 0.20 to 1.34 ± 0.26–1.49 ± 0.13 mM.

Bottom Line: The current knowledge regarding the effect of global climate change on rice-paddy methane (CH4) emissions is incomplete, partly because information is limited concerning the mechanism of the microbial response to elevated ground-level ozone (O3).We found that elevated ground-level O3 inhibited methanogenic activity and influenced the composition of paddy methanogenic communities, reducing the abundance and diversity of paddy methanogens by adversely affecting dominant groups, such as aceticlastic Methanosaeta, especially at the rice tillering stage.Our results indicated that continuously elevated ground-level O3 would negatively influence paddy methanogenic archaeal communities and its critical ecological function.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences Nanjing, 210008, Jiangsu Province P.R. China.

ABSTRACT
The current knowledge regarding the effect of global climate change on rice-paddy methane (CH4) emissions is incomplete, partly because information is limited concerning the mechanism of the microbial response to elevated ground-level ozone (O3). A field experiment was conducted in the China Ozone Free-Air Concentration Enrichment facility in a rice-wheat rotation system to investigate the responses of methanogenic archaeal communities to elevated ground-level O3 by culture-independent and -reliant approaches. We found that elevated ground-level O3 inhibited methanogenic activity and influenced the composition of paddy methanogenic communities, reducing the abundance and diversity of paddy methanogens by adversely affecting dominant groups, such as aceticlastic Methanosaeta, especially at the rice tillering stage. Our results indicated that continuously elevated ground-level O3 would negatively influence paddy methanogenic archaeal communities and its critical ecological function. These findings will contribute to a comprehensive understanding of the responses and feedbacks of paddy ecosystems to global climate change.

Show MeSH
Related in: MedlinePlus