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Effects of an extracted lectin from Citrullus colocynthis L. (Cucurbitaceae) on survival, digestion and energy reserves of Ectomyelois ceratoniae Zeller (Lepidoptera: Pyralidae).

Ramzi S, Sahragard A, Sendi JJ, Aalami A - Front Physiol (2013)

Bottom Line: Activities of all specific proteases decreased when larvae were fed on different concentrations of CCA except for aminopeptidase.Meanwhile, amount of storage macromolecules in the larvae fed on different concentrations of CCA statistically decreased vs. control.These results demonstrated that CCA could intervene in physiology of E. ceratoniae and survival of larvae.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Protection, Faculty of Agricultural Science, University of Guilan Rasht, Iran.

ABSTRACT
Lectins are the heterogeneous proteins in plants that serve as storage proteins via defensive mechanisms against herbivores. In the current study, a lectin was extracted and purified from seeds of Citrullus colocynthis by Sepharose 4B-Galactose and DEAE-cellulose fast flow chromatographies. Different concentrations of the lectin were added to artificial diet of Ectomyelois ceratoniae larvae finding out its effect on some biological parameters, digestive physiology and amount of storage macromolecules. It was found that CCA (C. colocynthis Agglutinin) increased life span from 23.44 days in control to 28.59 days in the treated individuals. Survival of larvae on control and CCA diets were 93.3 and 66.6%, respectively. Different concentrations of CCA significantly affected α-amylase and general proteolytic activities except for TAG-lipase activity. Activities of all specific proteases decreased when larvae were fed on different concentrations of CCA except for aminopeptidase. Meanwhile, amount of storage macromolecules in the larvae fed on different concentrations of CCA statistically decreased vs. control. These results demonstrated that CCA could intervene in physiology of E. ceratoniae and survival of larvae. Therefore, it can be taken into consideration in IPM of the pest through plant breeding programs.

No MeSH data available.


Related in: MedlinePlus

Effect of CCA on α-amylase activity in the treated larvae of E. ceratoniae. Different Letters show statistical differences (Tukey's test, p ≤ 0.05).
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Figure 2: Effect of CCA on α-amylase activity in the treated larvae of E. ceratoniae. Different Letters show statistical differences (Tukey's test, p ≤ 0.05).

Mentions: Larvae of E. ceratoniae were allowed to feed on the diet containing 0, 0.5, 1, and 2% of CCA from 1st to the first half of 5th larval instar to find out any changes in digestive enzyme activities. The α-amylase activity decreased statistically in the larvae fed on different concentrations of CCA (Figure 2). No statistical differences was observed regarding TAG-lipase activity in treated and control larvae in both enzymatic assay and gel electrophoresis (Figures 3A,B). In case of general proteolytic activity, no statistical differences was observed between control and larvae fed on CCA 0.5% but proteolytic activity in the larvae fed on CCA 2% sharply decreased and showed significant differences (Figure 4A). In gel electrophoresis, five proteolytic bands were observed in control that P3–P5 disappeared or their sharpness decreased in the larvae fed on CCA 2% (Figure 4B).


Effects of an extracted lectin from Citrullus colocynthis L. (Cucurbitaceae) on survival, digestion and energy reserves of Ectomyelois ceratoniae Zeller (Lepidoptera: Pyralidae).

Ramzi S, Sahragard A, Sendi JJ, Aalami A - Front Physiol (2013)

Effect of CCA on α-amylase activity in the treated larvae of E. ceratoniae. Different Letters show statistical differences (Tukey's test, p ≤ 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3824156&req=5

Figure 2: Effect of CCA on α-amylase activity in the treated larvae of E. ceratoniae. Different Letters show statistical differences (Tukey's test, p ≤ 0.05).
Mentions: Larvae of E. ceratoniae were allowed to feed on the diet containing 0, 0.5, 1, and 2% of CCA from 1st to the first half of 5th larval instar to find out any changes in digestive enzyme activities. The α-amylase activity decreased statistically in the larvae fed on different concentrations of CCA (Figure 2). No statistical differences was observed regarding TAG-lipase activity in treated and control larvae in both enzymatic assay and gel electrophoresis (Figures 3A,B). In case of general proteolytic activity, no statistical differences was observed between control and larvae fed on CCA 0.5% but proteolytic activity in the larvae fed on CCA 2% sharply decreased and showed significant differences (Figure 4A). In gel electrophoresis, five proteolytic bands were observed in control that P3–P5 disappeared or their sharpness decreased in the larvae fed on CCA 2% (Figure 4B).

Bottom Line: Activities of all specific proteases decreased when larvae were fed on different concentrations of CCA except for aminopeptidase.Meanwhile, amount of storage macromolecules in the larvae fed on different concentrations of CCA statistically decreased vs. control.These results demonstrated that CCA could intervene in physiology of E. ceratoniae and survival of larvae.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Protection, Faculty of Agricultural Science, University of Guilan Rasht, Iran.

ABSTRACT
Lectins are the heterogeneous proteins in plants that serve as storage proteins via defensive mechanisms against herbivores. In the current study, a lectin was extracted and purified from seeds of Citrullus colocynthis by Sepharose 4B-Galactose and DEAE-cellulose fast flow chromatographies. Different concentrations of the lectin were added to artificial diet of Ectomyelois ceratoniae larvae finding out its effect on some biological parameters, digestive physiology and amount of storage macromolecules. It was found that CCA (C. colocynthis Agglutinin) increased life span from 23.44 days in control to 28.59 days in the treated individuals. Survival of larvae on control and CCA diets were 93.3 and 66.6%, respectively. Different concentrations of CCA significantly affected α-amylase and general proteolytic activities except for TAG-lipase activity. Activities of all specific proteases decreased when larvae were fed on different concentrations of CCA except for aminopeptidase. Meanwhile, amount of storage macromolecules in the larvae fed on different concentrations of CCA statistically decreased vs. control. These results demonstrated that CCA could intervene in physiology of E. ceratoniae and survival of larvae. Therefore, it can be taken into consideration in IPM of the pest through plant breeding programs.

No MeSH data available.


Related in: MedlinePlus