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Effects of an extracted lectin from Citrullus colocynthis L. (Cucurbitaceae) on survival, digestion and energy reserves of Ectomyelois ceratoniae Zeller (Lepidoptera: Pyralidae).

Ramzi S, Sahragard A, Sendi JJ, Aalami A - Front Physiol (2013)

Bottom Line: Activities of all specific proteases decreased when larvae were fed on different concentrations of CCA except for aminopeptidase.Meanwhile, amount of storage macromolecules in the larvae fed on different concentrations of CCA statistically decreased vs. control.These results demonstrated that CCA could intervene in physiology of E. ceratoniae and survival of larvae.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Protection, Faculty of Agricultural Science, University of Guilan Rasht, Iran.

ABSTRACT
Lectins are the heterogeneous proteins in plants that serve as storage proteins via defensive mechanisms against herbivores. In the current study, a lectin was extracted and purified from seeds of Citrullus colocynthis by Sepharose 4B-Galactose and DEAE-cellulose fast flow chromatographies. Different concentrations of the lectin were added to artificial diet of Ectomyelois ceratoniae larvae finding out its effect on some biological parameters, digestive physiology and amount of storage macromolecules. It was found that CCA (C. colocynthis Agglutinin) increased life span from 23.44 days in control to 28.59 days in the treated individuals. Survival of larvae on control and CCA diets were 93.3 and 66.6%, respectively. Different concentrations of CCA significantly affected α-amylase and general proteolytic activities except for TAG-lipase activity. Activities of all specific proteases decreased when larvae were fed on different concentrations of CCA except for aminopeptidase. Meanwhile, amount of storage macromolecules in the larvae fed on different concentrations of CCA statistically decreased vs. control. These results demonstrated that CCA could intervene in physiology of E. ceratoniae and survival of larvae. Therefore, it can be taken into consideration in IPM of the pest through plant breeding programs.

No MeSH data available.


Related in: MedlinePlus

Purified CCA after column chromatography procedures. (A) Molecular weight. (B) Freezdried protein.
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Figure 1: Purified CCA after column chromatography procedures. (A) Molecular weight. (B) Freezdried protein.

Mentions: Purification process of the crude sample from C. colocynthis revealed a purified protein by molecular weight of 14.4 kDa (Figures 1). Involvement of CCA into artificial diet of E. ceratoniae caused statistical changes in life cycle, survival and pupal weight of the pest vs. control (Table 1). The pre-adult period increased to 28.59 days on larvae fed on the diet containing 2% CCA in comparison with control by reducing of survival to 66.6% (Table 1). Larval Feeding on different concentrations of CCA revealed a statistical reduction and abnormality in the pupal weight and morphology (Table 1).


Effects of an extracted lectin from Citrullus colocynthis L. (Cucurbitaceae) on survival, digestion and energy reserves of Ectomyelois ceratoniae Zeller (Lepidoptera: Pyralidae).

Ramzi S, Sahragard A, Sendi JJ, Aalami A - Front Physiol (2013)

Purified CCA after column chromatography procedures. (A) Molecular weight. (B) Freezdried protein.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3824156&req=5

Figure 1: Purified CCA after column chromatography procedures. (A) Molecular weight. (B) Freezdried protein.
Mentions: Purification process of the crude sample from C. colocynthis revealed a purified protein by molecular weight of 14.4 kDa (Figures 1). Involvement of CCA into artificial diet of E. ceratoniae caused statistical changes in life cycle, survival and pupal weight of the pest vs. control (Table 1). The pre-adult period increased to 28.59 days on larvae fed on the diet containing 2% CCA in comparison with control by reducing of survival to 66.6% (Table 1). Larval Feeding on different concentrations of CCA revealed a statistical reduction and abnormality in the pupal weight and morphology (Table 1).

Bottom Line: Activities of all specific proteases decreased when larvae were fed on different concentrations of CCA except for aminopeptidase.Meanwhile, amount of storage macromolecules in the larvae fed on different concentrations of CCA statistically decreased vs. control.These results demonstrated that CCA could intervene in physiology of E. ceratoniae and survival of larvae.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Protection, Faculty of Agricultural Science, University of Guilan Rasht, Iran.

ABSTRACT
Lectins are the heterogeneous proteins in plants that serve as storage proteins via defensive mechanisms against herbivores. In the current study, a lectin was extracted and purified from seeds of Citrullus colocynthis by Sepharose 4B-Galactose and DEAE-cellulose fast flow chromatographies. Different concentrations of the lectin were added to artificial diet of Ectomyelois ceratoniae larvae finding out its effect on some biological parameters, digestive physiology and amount of storage macromolecules. It was found that CCA (C. colocynthis Agglutinin) increased life span from 23.44 days in control to 28.59 days in the treated individuals. Survival of larvae on control and CCA diets were 93.3 and 66.6%, respectively. Different concentrations of CCA significantly affected α-amylase and general proteolytic activities except for TAG-lipase activity. Activities of all specific proteases decreased when larvae were fed on different concentrations of CCA except for aminopeptidase. Meanwhile, amount of storage macromolecules in the larvae fed on different concentrations of CCA statistically decreased vs. control. These results demonstrated that CCA could intervene in physiology of E. ceratoniae and survival of larvae. Therefore, it can be taken into consideration in IPM of the pest through plant breeding programs.

No MeSH data available.


Related in: MedlinePlus