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amoA-encoding archaea and thaumarchaeol in the lakes on the northeastern Qinghai-Tibetan Plateau, China.

Yang J, Jiang H, Dong H, Wang H, Wu G, Hou W, Liu W, Zhang C, Sun Y, Lai Z - Front Microbiol (2013)

Bottom Line: The results showed that the archaeal amoA gene was present in hypersaline lakes with salinity up to 160 g L(-) (1).Thaumarchaeol was present in all of the studied hypersaline lakes, even in those where no AEA amoA gene was observed.Future research is needed to determine the ecological function of AEA and possible sources of thaumarchaeol in the Qinghai-Tibetan hypersaline lakes.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences Wuhan, China ; Key Lab of Salt Lake Resources and Chemistry, Qinghai Institute of Salt Lakes, Chinese Academy of Sciences Xining, China.

ABSTRACT
All known ammonia-oxidizing archaea (AOA) belong to the phylum Thaumarchaeota within the domain Archaea. AOA possess the diagnostic amoA gene (encoding the alpha subunit of ammonia monooxygenase) and produce lipid biomarker thaumarchaeol. Although the abundance and diversity of amoA gene-encoding archaea (AEA) in freshwater lakes have been well-studied, little is known about AEA ecology in saline/hypersaline lakes. In this study, the distribution of the archaeal amoA gene and thaumarchaeol were investigated in nine Qinghai-Tibetan lakes with a salinity range from freshwater to salt-saturation (salinity: 325 g L(-) (1)). The results showed that the archaeal amoA gene was present in hypersaline lakes with salinity up to 160 g L(-) (1). The archaeal amoA gene diversity in Tibetan lakes was different from those in other lakes worldwide, suggesting Tibetan lakes (high elevation, strong ultraviolet, and dry climate) may host a unique AEA population of different evolutionary origin from those in other lakes. Thaumarchaeol was present in all of the studied hypersaline lakes, even in those where no AEA amoA gene was observed. Future research is needed to determine the ecological function of AEA and possible sources of thaumarchaeol in the Qinghai-Tibetan hypersaline lakes.

No MeSH data available.


Related in: MedlinePlus

(A) Clustering of the different amoA gene clone libraries based on ΔCxy values determined from the LIBSHUFF analysis. The tree was constructed with the unweighted-pair group method using average linkages in MEGA 5. The parameter ΔCxy in the LIBSHUFF analysis represents the difference in coverage of any two clone libraries (the larger ΔCxy, the greater dissimilarity between the given clone libraries). The software for the analysis was available at (B) Schematic figure showing the frequencies of OTUs (at 98% nucleotide cutoff) affiliated with major phylogenetic groups in the amoA gene clone libraries.
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Figure 4: (A) Clustering of the different amoA gene clone libraries based on ΔCxy values determined from the LIBSHUFF analysis. The tree was constructed with the unweighted-pair group method using average linkages in MEGA 5. The parameter ΔCxy in the LIBSHUFF analysis represents the difference in coverage of any two clone libraries (the larger ΔCxy, the greater dissimilarity between the given clone libraries). The software for the analysis was available at (B) Schematic figure showing the frequencies of OTUs (at 98% nucleotide cutoff) affiliated with major phylogenetic groups in the amoA gene clone libraries.

Mentions: The LIBSHUFF analysis showed that the archaeal amoA gene clone libraries were grouped into two separate clusters (one each for the waters and sediments, respectively; P-value < 0.01; Figure 4A).


amoA-encoding archaea and thaumarchaeol in the lakes on the northeastern Qinghai-Tibetan Plateau, China.

Yang J, Jiang H, Dong H, Wang H, Wu G, Hou W, Liu W, Zhang C, Sun Y, Lai Z - Front Microbiol (2013)

(A) Clustering of the different amoA gene clone libraries based on ΔCxy values determined from the LIBSHUFF analysis. The tree was constructed with the unweighted-pair group method using average linkages in MEGA 5. The parameter ΔCxy in the LIBSHUFF analysis represents the difference in coverage of any two clone libraries (the larger ΔCxy, the greater dissimilarity between the given clone libraries). The software for the analysis was available at (B) Schematic figure showing the frequencies of OTUs (at 98% nucleotide cutoff) affiliated with major phylogenetic groups in the amoA gene clone libraries.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3824093&req=5

Figure 4: (A) Clustering of the different amoA gene clone libraries based on ΔCxy values determined from the LIBSHUFF analysis. The tree was constructed with the unweighted-pair group method using average linkages in MEGA 5. The parameter ΔCxy in the LIBSHUFF analysis represents the difference in coverage of any two clone libraries (the larger ΔCxy, the greater dissimilarity between the given clone libraries). The software for the analysis was available at (B) Schematic figure showing the frequencies of OTUs (at 98% nucleotide cutoff) affiliated with major phylogenetic groups in the amoA gene clone libraries.
Mentions: The LIBSHUFF analysis showed that the archaeal amoA gene clone libraries were grouped into two separate clusters (one each for the waters and sediments, respectively; P-value < 0.01; Figure 4A).

Bottom Line: The results showed that the archaeal amoA gene was present in hypersaline lakes with salinity up to 160 g L(-) (1).Thaumarchaeol was present in all of the studied hypersaline lakes, even in those where no AEA amoA gene was observed.Future research is needed to determine the ecological function of AEA and possible sources of thaumarchaeol in the Qinghai-Tibetan hypersaline lakes.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences Wuhan, China ; Key Lab of Salt Lake Resources and Chemistry, Qinghai Institute of Salt Lakes, Chinese Academy of Sciences Xining, China.

ABSTRACT
All known ammonia-oxidizing archaea (AOA) belong to the phylum Thaumarchaeota within the domain Archaea. AOA possess the diagnostic amoA gene (encoding the alpha subunit of ammonia monooxygenase) and produce lipid biomarker thaumarchaeol. Although the abundance and diversity of amoA gene-encoding archaea (AEA) in freshwater lakes have been well-studied, little is known about AEA ecology in saline/hypersaline lakes. In this study, the distribution of the archaeal amoA gene and thaumarchaeol were investigated in nine Qinghai-Tibetan lakes with a salinity range from freshwater to salt-saturation (salinity: 325 g L(-) (1)). The results showed that the archaeal amoA gene was present in hypersaline lakes with salinity up to 160 g L(-) (1). The archaeal amoA gene diversity in Tibetan lakes was different from those in other lakes worldwide, suggesting Tibetan lakes (high elevation, strong ultraviolet, and dry climate) may host a unique AEA population of different evolutionary origin from those in other lakes. Thaumarchaeol was present in all of the studied hypersaline lakes, even in those where no AEA amoA gene was observed. Future research is needed to determine the ecological function of AEA and possible sources of thaumarchaeol in the Qinghai-Tibetan hypersaline lakes.

No MeSH data available.


Related in: MedlinePlus