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amoA-encoding archaea and thaumarchaeol in the lakes on the northeastern Qinghai-Tibetan Plateau, China.

Yang J, Jiang H, Dong H, Wang H, Wu G, Hou W, Liu W, Zhang C, Sun Y, Lai Z - Front Microbiol (2013)

Bottom Line: The results showed that the archaeal amoA gene was present in hypersaline lakes with salinity up to 160 g L(-) (1).Thaumarchaeol was present in all of the studied hypersaline lakes, even in those where no AEA amoA gene was observed.Future research is needed to determine the ecological function of AEA and possible sources of thaumarchaeol in the Qinghai-Tibetan hypersaline lakes.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences Wuhan, China ; Key Lab of Salt Lake Resources and Chemistry, Qinghai Institute of Salt Lakes, Chinese Academy of Sciences Xining, China.

ABSTRACT
All known ammonia-oxidizing archaea (AOA) belong to the phylum Thaumarchaeota within the domain Archaea. AOA possess the diagnostic amoA gene (encoding the alpha subunit of ammonia monooxygenase) and produce lipid biomarker thaumarchaeol. Although the abundance and diversity of amoA gene-encoding archaea (AEA) in freshwater lakes have been well-studied, little is known about AEA ecology in saline/hypersaline lakes. In this study, the distribution of the archaeal amoA gene and thaumarchaeol were investigated in nine Qinghai-Tibetan lakes with a salinity range from freshwater to salt-saturation (salinity: 325 g L(-) (1)). The results showed that the archaeal amoA gene was present in hypersaline lakes with salinity up to 160 g L(-) (1). The archaeal amoA gene diversity in Tibetan lakes was different from those in other lakes worldwide, suggesting Tibetan lakes (high elevation, strong ultraviolet, and dry climate) may host a unique AEA population of different evolutionary origin from those in other lakes. Thaumarchaeol was present in all of the studied hypersaline lakes, even in those where no AEA amoA gene was observed. Future research is needed to determine the ecological function of AEA and possible sources of thaumarchaeol in the Qinghai-Tibetan hypersaline lakes.

No MeSH data available.


Related in: MedlinePlus

Scatter plot showing a correlation between sediment amoA gene copies and thaumarchaeol.
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Figure 3: Scatter plot showing a correlation between sediment amoA gene copies and thaumarchaeol.

Mentions: Total archaeal 16S rRNA gene abundance ranged from 3.86 × 104 to 4.15 × 106 copies mL-1 and from 1.66 × 105 to 2.33 × 109 copies g-1 for the waters and sediments, respectively (Figure 2). The amoA gene abundance ranged from 2.81 × 104–3.07 × 104 copies mL-1 and 7.34 × 104–4.13 × 106copies g-1 for the waters and sediments, respectively (Figure 2). The highest water and sediment 16S rRNA archaeal abundances were observed in the water of Tuosu Lake (4.15 × 106copies mL-1) and the sediment of Qinghai Lake (2.33 × 109 copies g-1); whereas the highest water and sediment archaeal amoA gene abundances were observed in the water of Gahai Lake 1 (3.07 × 104 copies mL-1) and the sediment of Tuosu Lake (4.13 × 106 copies g-1). In addition, the amoA gene abundance exhibited a significant correlation (CL: r = 0.879, P = 0.009; PL: r = 0.928, P = 0.003) with thaumarchaeol abundance for the sediment samples (except Lake Chaka, where no amoA gene abundance data were available; Figure 3).


amoA-encoding archaea and thaumarchaeol in the lakes on the northeastern Qinghai-Tibetan Plateau, China.

Yang J, Jiang H, Dong H, Wang H, Wu G, Hou W, Liu W, Zhang C, Sun Y, Lai Z - Front Microbiol (2013)

Scatter plot showing a correlation between sediment amoA gene copies and thaumarchaeol.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3824093&req=5

Figure 3: Scatter plot showing a correlation between sediment amoA gene copies and thaumarchaeol.
Mentions: Total archaeal 16S rRNA gene abundance ranged from 3.86 × 104 to 4.15 × 106 copies mL-1 and from 1.66 × 105 to 2.33 × 109 copies g-1 for the waters and sediments, respectively (Figure 2). The amoA gene abundance ranged from 2.81 × 104–3.07 × 104 copies mL-1 and 7.34 × 104–4.13 × 106copies g-1 for the waters and sediments, respectively (Figure 2). The highest water and sediment 16S rRNA archaeal abundances were observed in the water of Tuosu Lake (4.15 × 106copies mL-1) and the sediment of Qinghai Lake (2.33 × 109 copies g-1); whereas the highest water and sediment archaeal amoA gene abundances were observed in the water of Gahai Lake 1 (3.07 × 104 copies mL-1) and the sediment of Tuosu Lake (4.13 × 106 copies g-1). In addition, the amoA gene abundance exhibited a significant correlation (CL: r = 0.879, P = 0.009; PL: r = 0.928, P = 0.003) with thaumarchaeol abundance for the sediment samples (except Lake Chaka, where no amoA gene abundance data were available; Figure 3).

Bottom Line: The results showed that the archaeal amoA gene was present in hypersaline lakes with salinity up to 160 g L(-) (1).Thaumarchaeol was present in all of the studied hypersaline lakes, even in those where no AEA amoA gene was observed.Future research is needed to determine the ecological function of AEA and possible sources of thaumarchaeol in the Qinghai-Tibetan hypersaline lakes.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences Wuhan, China ; Key Lab of Salt Lake Resources and Chemistry, Qinghai Institute of Salt Lakes, Chinese Academy of Sciences Xining, China.

ABSTRACT
All known ammonia-oxidizing archaea (AOA) belong to the phylum Thaumarchaeota within the domain Archaea. AOA possess the diagnostic amoA gene (encoding the alpha subunit of ammonia monooxygenase) and produce lipid biomarker thaumarchaeol. Although the abundance and diversity of amoA gene-encoding archaea (AEA) in freshwater lakes have been well-studied, little is known about AEA ecology in saline/hypersaline lakes. In this study, the distribution of the archaeal amoA gene and thaumarchaeol were investigated in nine Qinghai-Tibetan lakes with a salinity range from freshwater to salt-saturation (salinity: 325 g L(-) (1)). The results showed that the archaeal amoA gene was present in hypersaline lakes with salinity up to 160 g L(-) (1). The archaeal amoA gene diversity in Tibetan lakes was different from those in other lakes worldwide, suggesting Tibetan lakes (high elevation, strong ultraviolet, and dry climate) may host a unique AEA population of different evolutionary origin from those in other lakes. Thaumarchaeol was present in all of the studied hypersaline lakes, even in those where no AEA amoA gene was observed. Future research is needed to determine the ecological function of AEA and possible sources of thaumarchaeol in the Qinghai-Tibetan hypersaline lakes.

No MeSH data available.


Related in: MedlinePlus