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amoA-encoding archaea and thaumarchaeol in the lakes on the northeastern Qinghai-Tibetan Plateau, China.

Yang J, Jiang H, Dong H, Wang H, Wu G, Hou W, Liu W, Zhang C, Sun Y, Lai Z - Front Microbiol (2013)

Bottom Line: The results showed that the archaeal amoA gene was present in hypersaline lakes with salinity up to 160 g L(-) (1).Thaumarchaeol was present in all of the studied hypersaline lakes, even in those where no AEA amoA gene was observed.Future research is needed to determine the ecological function of AEA and possible sources of thaumarchaeol in the Qinghai-Tibetan hypersaline lakes.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences Wuhan, China ; Key Lab of Salt Lake Resources and Chemistry, Qinghai Institute of Salt Lakes, Chinese Academy of Sciences Xining, China.

ABSTRACT
All known ammonia-oxidizing archaea (AOA) belong to the phylum Thaumarchaeota within the domain Archaea. AOA possess the diagnostic amoA gene (encoding the alpha subunit of ammonia monooxygenase) and produce lipid biomarker thaumarchaeol. Although the abundance and diversity of amoA gene-encoding archaea (AEA) in freshwater lakes have been well-studied, little is known about AEA ecology in saline/hypersaline lakes. In this study, the distribution of the archaeal amoA gene and thaumarchaeol were investigated in nine Qinghai-Tibetan lakes with a salinity range from freshwater to salt-saturation (salinity: 325 g L(-) (1)). The results showed that the archaeal amoA gene was present in hypersaline lakes with salinity up to 160 g L(-) (1). The archaeal amoA gene diversity in Tibetan lakes was different from those in other lakes worldwide, suggesting Tibetan lakes (high elevation, strong ultraviolet, and dry climate) may host a unique AEA population of different evolutionary origin from those in other lakes. Thaumarchaeol was present in all of the studied hypersaline lakes, even in those where no AEA amoA gene was observed. Future research is needed to determine the ecological function of AEA and possible sources of thaumarchaeol in the Qinghai-Tibetan hypersaline lakes.

No MeSH data available.


Related in: MedlinePlus

The abundances (copies per gram of sediment or copies per milliliter of water) of total archaeal 16S rRNA and amoA genes and thaumarchaeol concentrations (nanogram per liter of water and nanogram per gram of sediment). Panels (A) and (B) are for waters and sediments, respectively.
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Figure 2: The abundances (copies per gram of sediment or copies per milliliter of water) of total archaeal 16S rRNA and amoA genes and thaumarchaeol concentrations (nanogram per liter of water and nanogram per gram of sediment). Panels (A) and (B) are for waters and sediments, respectively.

Mentions: In the lake waters, thaumarchaeol concentration ranged from 0.0 to 0.3 ng L-1 for CLs and from 0.0 to 0.5 ng L-1 for PLs; in the lake sediments, thaumarchaeol concentrations ranged from 0.1 to 25.2 ng g-1 for CLs and 0.5 to 37.5 ng g-1 for PLs (Figure 2). The highest thaumarchaeol concentrations were observed in the sediment of Tuosu Lake (salinity: 31 g L-1): 25.2 and 37.5 ng g-1 for CLs and PLs, respectively (Figure 2).


amoA-encoding archaea and thaumarchaeol in the lakes on the northeastern Qinghai-Tibetan Plateau, China.

Yang J, Jiang H, Dong H, Wang H, Wu G, Hou W, Liu W, Zhang C, Sun Y, Lai Z - Front Microbiol (2013)

The abundances (copies per gram of sediment or copies per milliliter of water) of total archaeal 16S rRNA and amoA genes and thaumarchaeol concentrations (nanogram per liter of water and nanogram per gram of sediment). Panels (A) and (B) are for waters and sediments, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3824093&req=5

Figure 2: The abundances (copies per gram of sediment or copies per milliliter of water) of total archaeal 16S rRNA and amoA genes and thaumarchaeol concentrations (nanogram per liter of water and nanogram per gram of sediment). Panels (A) and (B) are for waters and sediments, respectively.
Mentions: In the lake waters, thaumarchaeol concentration ranged from 0.0 to 0.3 ng L-1 for CLs and from 0.0 to 0.5 ng L-1 for PLs; in the lake sediments, thaumarchaeol concentrations ranged from 0.1 to 25.2 ng g-1 for CLs and 0.5 to 37.5 ng g-1 for PLs (Figure 2). The highest thaumarchaeol concentrations were observed in the sediment of Tuosu Lake (salinity: 31 g L-1): 25.2 and 37.5 ng g-1 for CLs and PLs, respectively (Figure 2).

Bottom Line: The results showed that the archaeal amoA gene was present in hypersaline lakes with salinity up to 160 g L(-) (1).Thaumarchaeol was present in all of the studied hypersaline lakes, even in those where no AEA amoA gene was observed.Future research is needed to determine the ecological function of AEA and possible sources of thaumarchaeol in the Qinghai-Tibetan hypersaline lakes.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences Wuhan, China ; Key Lab of Salt Lake Resources and Chemistry, Qinghai Institute of Salt Lakes, Chinese Academy of Sciences Xining, China.

ABSTRACT
All known ammonia-oxidizing archaea (AOA) belong to the phylum Thaumarchaeota within the domain Archaea. AOA possess the diagnostic amoA gene (encoding the alpha subunit of ammonia monooxygenase) and produce lipid biomarker thaumarchaeol. Although the abundance and diversity of amoA gene-encoding archaea (AEA) in freshwater lakes have been well-studied, little is known about AEA ecology in saline/hypersaline lakes. In this study, the distribution of the archaeal amoA gene and thaumarchaeol were investigated in nine Qinghai-Tibetan lakes with a salinity range from freshwater to salt-saturation (salinity: 325 g L(-) (1)). The results showed that the archaeal amoA gene was present in hypersaline lakes with salinity up to 160 g L(-) (1). The archaeal amoA gene diversity in Tibetan lakes was different from those in other lakes worldwide, suggesting Tibetan lakes (high elevation, strong ultraviolet, and dry climate) may host a unique AEA population of different evolutionary origin from those in other lakes. Thaumarchaeol was present in all of the studied hypersaline lakes, even in those where no AEA amoA gene was observed. Future research is needed to determine the ecological function of AEA and possible sources of thaumarchaeol in the Qinghai-Tibetan hypersaline lakes.

No MeSH data available.


Related in: MedlinePlus