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Acyl-homoserine lactone-based quorum sensing in the Roseobacter clade: complex cell-to-cell communication controls multiple physiologies.

Cude WN, Buchan A - Front Microbiol (2013)

Bottom Line: Recent studies suggest that members of the abundant marine Roseobacter lineage possess AHL-based QS systems and are environmentally relevant models for relating QS to ecological success.As reviewed here, these studies suggest that the roles of QS in roseobacters are varied and complex.An analysis of the 43 publically available Roseobacter genomes shows conservation of QS protein sequences and overall gene topologies, providing support for the hypothesis that QS is a conserved and widespread trait in the clade.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, University of Tennessee Knoxville, TN, USA.

ABSTRACT
Bacteria have been widely reported to use quorum sensing (QS) systems, which employ small diffusible metabolites to coordinate gene expression in a population density dependent manner. In Proteobacteria, the most commonly described QS signaling molecules are N-acyl-homoserine lactones (AHLs). Recent studies suggest that members of the abundant marine Roseobacter lineage possess AHL-based QS systems and are environmentally relevant models for relating QS to ecological success. As reviewed here, these studies suggest that the roles of QS in roseobacters are varied and complex. An analysis of the 43 publically available Roseobacter genomes shows conservation of QS protein sequences and overall gene topologies, providing support for the hypothesis that QS is a conserved and widespread trait in the clade.

No MeSH data available.


Related in: MedlinePlus

The gene orientation of all putative luxRI operons in available Roseobacter genomes. Green arrows represent AHL synthase encoding genes (luxI), orange arrows represent AHL binding response regulators (luxR), and black arrows represent adjacent genes. Abbreviations used: Crotonyl CoA, Crotonyl CoA reductase; HK, histidine kinase; HK/RR, hybrid histidine kinase/response regulator; HK/REC, histidine kinase with REC domain; CHP, conserved hypothetical protein; RND, RND multidrug efflux pump; Sig B, sigma B factor; SCD, short chain dehydrogenase; Trans., transposase; L17, L17 component of the 50S ribosomal protein; 2CRS, two-component regulatory system; TctA, TctA family transmembrane transporter; mdlB, mandelate dehydrogenase mdlB; spαβ, α and β subunits of sulfopyruvate decarboxylase. Rx and Ix designations above the response regulators and AHL synthases indicate their corresponding phylogentic subgroupings in Figures 1A,B, respectively. Those without Rx and Ix designations indicate unique sequences not found in the conserved groupings. The corresponding genomes that contain these topologies can be found in Table A1.
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Figure 2: The gene orientation of all putative luxRI operons in available Roseobacter genomes. Green arrows represent AHL synthase encoding genes (luxI), orange arrows represent AHL binding response regulators (luxR), and black arrows represent adjacent genes. Abbreviations used: Crotonyl CoA, Crotonyl CoA reductase; HK, histidine kinase; HK/RR, hybrid histidine kinase/response regulator; HK/REC, histidine kinase with REC domain; CHP, conserved hypothetical protein; RND, RND multidrug efflux pump; Sig B, sigma B factor; SCD, short chain dehydrogenase; Trans., transposase; L17, L17 component of the 50S ribosomal protein; 2CRS, two-component regulatory system; TctA, TctA family transmembrane transporter; mdlB, mandelate dehydrogenase mdlB; spαβ, α and β subunits of sulfopyruvate decarboxylase. Rx and Ix designations above the response regulators and AHL synthases indicate their corresponding phylogentic subgroupings in Figures 1A,B, respectively. Those without Rx and Ix designations indicate unique sequences not found in the conserved groupings. The corresponding genomes that contain these topologies can be found in Table A1.

Mentions: Genome analysis demonstrates that multiple conserved QS gene topologies are present within sequenced roseobacters, allowing for classification by sequence similarity and gene orientation (Figure 2 and Table A1). The most conserved gene topologies are the A and B groups, of which 28 different Roseobacter genomes contain one of the orientations, and three Ruegeria genomes contain both. Genomes that contain the A topology have highly similar LuxI and LuxR sequences (>63 and >70% similarity, respectively) and its presence in three different roseobacter subclades (defined in Newton et al., 2010) may be suggestive of HGT (Figures 1A,B). Genomes with topology A share a Trigger Factor (TF) encoding gene downstream from luxRI (Figure 2). The location of this TF is conserved in seven genomes. Though the function has not been examined in roseobacters, in Vibrio cholera, TFs play a role in the folding and secretion of proteins (Ludlam et al., 2004). The LuxI and LuxR of the A topology have been designated Iα and Rα, respectively (Figure 2).


Acyl-homoserine lactone-based quorum sensing in the Roseobacter clade: complex cell-to-cell communication controls multiple physiologies.

Cude WN, Buchan A - Front Microbiol (2013)

The gene orientation of all putative luxRI operons in available Roseobacter genomes. Green arrows represent AHL synthase encoding genes (luxI), orange arrows represent AHL binding response regulators (luxR), and black arrows represent adjacent genes. Abbreviations used: Crotonyl CoA, Crotonyl CoA reductase; HK, histidine kinase; HK/RR, hybrid histidine kinase/response regulator; HK/REC, histidine kinase with REC domain; CHP, conserved hypothetical protein; RND, RND multidrug efflux pump; Sig B, sigma B factor; SCD, short chain dehydrogenase; Trans., transposase; L17, L17 component of the 50S ribosomal protein; 2CRS, two-component regulatory system; TctA, TctA family transmembrane transporter; mdlB, mandelate dehydrogenase mdlB; spαβ, α and β subunits of sulfopyruvate decarboxylase. Rx and Ix designations above the response regulators and AHL synthases indicate their corresponding phylogentic subgroupings in Figures 1A,B, respectively. Those without Rx and Ix designations indicate unique sequences not found in the conserved groupings. The corresponding genomes that contain these topologies can be found in Table A1.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3824088&req=5

Figure 2: The gene orientation of all putative luxRI operons in available Roseobacter genomes. Green arrows represent AHL synthase encoding genes (luxI), orange arrows represent AHL binding response regulators (luxR), and black arrows represent adjacent genes. Abbreviations used: Crotonyl CoA, Crotonyl CoA reductase; HK, histidine kinase; HK/RR, hybrid histidine kinase/response regulator; HK/REC, histidine kinase with REC domain; CHP, conserved hypothetical protein; RND, RND multidrug efflux pump; Sig B, sigma B factor; SCD, short chain dehydrogenase; Trans., transposase; L17, L17 component of the 50S ribosomal protein; 2CRS, two-component regulatory system; TctA, TctA family transmembrane transporter; mdlB, mandelate dehydrogenase mdlB; spαβ, α and β subunits of sulfopyruvate decarboxylase. Rx and Ix designations above the response regulators and AHL synthases indicate their corresponding phylogentic subgroupings in Figures 1A,B, respectively. Those without Rx and Ix designations indicate unique sequences not found in the conserved groupings. The corresponding genomes that contain these topologies can be found in Table A1.
Mentions: Genome analysis demonstrates that multiple conserved QS gene topologies are present within sequenced roseobacters, allowing for classification by sequence similarity and gene orientation (Figure 2 and Table A1). The most conserved gene topologies are the A and B groups, of which 28 different Roseobacter genomes contain one of the orientations, and three Ruegeria genomes contain both. Genomes that contain the A topology have highly similar LuxI and LuxR sequences (>63 and >70% similarity, respectively) and its presence in three different roseobacter subclades (defined in Newton et al., 2010) may be suggestive of HGT (Figures 1A,B). Genomes with topology A share a Trigger Factor (TF) encoding gene downstream from luxRI (Figure 2). The location of this TF is conserved in seven genomes. Though the function has not been examined in roseobacters, in Vibrio cholera, TFs play a role in the folding and secretion of proteins (Ludlam et al., 2004). The LuxI and LuxR of the A topology have been designated Iα and Rα, respectively (Figure 2).

Bottom Line: Recent studies suggest that members of the abundant marine Roseobacter lineage possess AHL-based QS systems and are environmentally relevant models for relating QS to ecological success.As reviewed here, these studies suggest that the roles of QS in roseobacters are varied and complex.An analysis of the 43 publically available Roseobacter genomes shows conservation of QS protein sequences and overall gene topologies, providing support for the hypothesis that QS is a conserved and widespread trait in the clade.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, University of Tennessee Knoxville, TN, USA.

ABSTRACT
Bacteria have been widely reported to use quorum sensing (QS) systems, which employ small diffusible metabolites to coordinate gene expression in a population density dependent manner. In Proteobacteria, the most commonly described QS signaling molecules are N-acyl-homoserine lactones (AHLs). Recent studies suggest that members of the abundant marine Roseobacter lineage possess AHL-based QS systems and are environmentally relevant models for relating QS to ecological success. As reviewed here, these studies suggest that the roles of QS in roseobacters are varied and complex. An analysis of the 43 publically available Roseobacter genomes shows conservation of QS protein sequences and overall gene topologies, providing support for the hypothesis that QS is a conserved and widespread trait in the clade.

No MeSH data available.


Related in: MedlinePlus