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Decreased survival of newborn neurons in the dorsal hippocampus after neonatal LPS exposure in mice.

Järlestedt K, Naylor AS, Dean J, Hagberg H, Mallard C - Neuroscience (2013)

Bottom Line: In contrast, the number of neurons and astrocytes that were born after LPS injection were reduced on P 41.The LPS-induced reduction in cell numbers was specific for the dorsal hippocampus.The reduction in cell survival could be attributed to less cell survival in the dorsal hippocampus, but had no effect on learning and memory in the young adult.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

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Assessment of cell proliferation in the dentate gyrus 48 h after injection of LPS. (A, C, D) Proliferating cells in the granule cell layer, labeled with BrdU at P11, 48 h post injection of LPS, were not decreased after LPS injection compared to saline-injected animals. (B–D) Proliferating cells in the Hilus were not decreased after LPS injection compared to saline-injected animals. Data are represented as group means ± SEM. Scale bars = 100 μm.
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f0020: Assessment of cell proliferation in the dentate gyrus 48 h after injection of LPS. (A, C, D) Proliferating cells in the granule cell layer, labeled with BrdU at P11, 48 h post injection of LPS, were not decreased after LPS injection compared to saline-injected animals. (B–D) Proliferating cells in the Hilus were not decreased after LPS injection compared to saline-injected animals. Data are represented as group means ± SEM. Scale bars = 100 μm.

Mentions: To further understand the effect of cell survival on cells born after LPS, we analyzed the effect of LPS administration on cell division and proliferation. Mice (n = 10 per group) were injected with LPS or saline at P9 and BrdU was administered at P11, with the animals sacrificed 2 h after the last BrdU injection (Fig. 1C). We found no effect of LPS on the total number of newborn proliferating cells in the GCL (saline: 6237 ± 541 vs. LPS: 5730 ± 233; p > 0.05, Fig. 4A, B) or in the hilus (saline: 3047 ± 210 vs. LPS: 3402 ± 77; p > 0.05, Fig. 4C, D).


Decreased survival of newborn neurons in the dorsal hippocampus after neonatal LPS exposure in mice.

Järlestedt K, Naylor AS, Dean J, Hagberg H, Mallard C - Neuroscience (2013)

Assessment of cell proliferation in the dentate gyrus 48 h after injection of LPS. (A, C, D) Proliferating cells in the granule cell layer, labeled with BrdU at P11, 48 h post injection of LPS, were not decreased after LPS injection compared to saline-injected animals. (B–D) Proliferating cells in the Hilus were not decreased after LPS injection compared to saline-injected animals. Data are represented as group means ± SEM. Scale bars = 100 μm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3824076&req=5

f0020: Assessment of cell proliferation in the dentate gyrus 48 h after injection of LPS. (A, C, D) Proliferating cells in the granule cell layer, labeled with BrdU at P11, 48 h post injection of LPS, were not decreased after LPS injection compared to saline-injected animals. (B–D) Proliferating cells in the Hilus were not decreased after LPS injection compared to saline-injected animals. Data are represented as group means ± SEM. Scale bars = 100 μm.
Mentions: To further understand the effect of cell survival on cells born after LPS, we analyzed the effect of LPS administration on cell division and proliferation. Mice (n = 10 per group) were injected with LPS or saline at P9 and BrdU was administered at P11, with the animals sacrificed 2 h after the last BrdU injection (Fig. 1C). We found no effect of LPS on the total number of newborn proliferating cells in the GCL (saline: 6237 ± 541 vs. LPS: 5730 ± 233; p > 0.05, Fig. 4A, B) or in the hilus (saline: 3047 ± 210 vs. LPS: 3402 ± 77; p > 0.05, Fig. 4C, D).

Bottom Line: In contrast, the number of neurons and astrocytes that were born after LPS injection were reduced on P 41.The LPS-induced reduction in cell numbers was specific for the dorsal hippocampus.The reduction in cell survival could be attributed to less cell survival in the dorsal hippocampus, but had no effect on learning and memory in the young adult.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

Show MeSH
Related in: MedlinePlus