Decreased survival of newborn neurons in the dorsal hippocampus after neonatal LPS exposure in mice.
Bottom Line: In contrast, the number of neurons and astrocytes that were born after LPS injection were reduced on P 41.The LPS-induced reduction in cell numbers was specific for the dorsal hippocampus.The reduction in cell survival could be attributed to less cell survival in the dorsal hippocampus, but had no effect on learning and memory in the young adult.
Affiliation: Department of Physiology, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.Show MeSH
Related in: MedlinePlus
Mentions: To investigate the effect of LPS on hippocampal cell survival in the young postnatal mouse, pups (n = 10 in each group) were injected with a single dose of LPS (1 mg/kg, ultra pure Escherichia coli LPS 055:B5, Sigma–Aldrich Sweden AB Stockholm, Sweden) or saline i.p. at postnatal day (P) 9. Cells undergoing division were labeled at either P8 (24 h prior to LPS injection), to investigate the effect of LPS on cells undergoing division prior to inflammation or at P11 (48 h post LPS injection) to investigate cells undergoing cell division post injection of LPS. BrdU (50 mg/kg/injection, Sigma–Aldrich) was injected in two pulses during the first few hours of the dark phase and during the middle of the dark phase (10 am and 2 pm, respectively). Animals were then left in their home cages and were sacrificed 30 days post BrdU injection (Fig. 1A, B).
Affiliation: Department of Physiology, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.