A solid state fungal fermentation-based strategy for the hydrolysis of wheat straw.
Bottom Line: The addition of yeast extract (0.5% w/v) and minerals significantly improved the cellulase production, to 24.0 ± 1.76 U/g.The hydrolysis of the fermented wheat straw using the fungal culture filtrate or commercial cellulase Ctec2 was performed, resulting in 4.34 and 3.13 g/L glucose respectively.It indicated that the fungal filtrate harvested from the fungal fermentation of wheat straw contained a more suitable enzyme mixture than the commercial cellulase.
Affiliation: School of Biosciences, Sutton Bonington Campus, University of Nottingham, Loughborough, Leicestershire LE12 5RD, United Kingdom.Show MeSH
Mentions: After the solid state fungal fermentation, the fungal extract (enzyme solution) was collected and used as the cellulase solution for the enzymatic hydrolysis of the fermented wheat straw, as shown in Fig. 1. Fig. 6 presents the glucose concentration profiles of the wheat straw hydrolysis catalyzed by both the commercial cellulase Ctec2 (Novozymes) and the fungal extract (from the SSF using autoclaved wheat straw, 0.5% YE with mineral). Two substrates, fermented wheat straw and autoclaved wheat straw before the fungal fermentations were compared in order to investigate the impact of SSF itself. In the first 22 h, no significant difference was observed between these experiments. Glucose was released with a fast speed of around 0.4 g/L/h at the first 6 h. After 22 h the hydrolysis using Ctec2 stopped glucose generation, resulting in around 3.13 g/L glucose. Meanwhile, the hydrolyses using the fungal extract and mixture of fungal extract /Ctec2 continued until around 4.3 g/L (using fermented wheat straw) and 3.6 g/L (using autoclaved wheat straw) glucose were liberated into the hydrolysates. These results suggested that although the fungal extract had a lower detected cellulase activity, it performed the same or even better than the Ctec2 solution.
Affiliation: School of Biosciences, Sutton Bonington Campus, University of Nottingham, Loughborough, Leicestershire LE12 5RD, United Kingdom.