A solid state fungal fermentation-based strategy for the hydrolysis of wheat straw.
Bottom Line: The addition of yeast extract (0.5% w/v) and minerals significantly improved the cellulase production, to 24.0 ± 1.76 U/g.The hydrolysis of the fermented wheat straw using the fungal culture filtrate or commercial cellulase Ctec2 was performed, resulting in 4.34 and 3.13 g/L glucose respectively.It indicated that the fungal filtrate harvested from the fungal fermentation of wheat straw contained a more suitable enzyme mixture than the commercial cellulase.
Affiliation: School of Biosciences, Sutton Bonington Campus, University of Nottingham, Loughborough, Leicestershire LE12 5RD, United Kingdom.Show MeSH
Mentions: To further improve cellulase generation, 0.5% yeast extract (YE) was added to wheat straw to investigate the impact of adding extra nutrients on enzyme production. As shown in Table 3 and Fig. 5, the cellulase activities increased to 15.1 and 18.6 U/g after 3 and 5 days culture, respectively. More promisingly, when a mineral solution was added, the cellulase activities achieved 16.7 and 24.0 U/g after 3 and 5 days culture, respectively. When 5% YE was added, a cellulase activity of 38.8 U/g was obtained. This is in agreement with various studies that the addition of nutrients could improve cellulase production (Gao et al., 2008; Kachlishvili et al., 2006). However, Ncube et al. (2012) cultured A. niger FGSCA733 on a Jatropha curcas-based substrate. It was found that the addition of nitrogen sources did not enhance cellulase production. A. niger was found to grow on a medium containing YE and minerals (0.5%, in a liquid culture), but the cellulase activity was only 0.15 ± 0.01 U/ml after 5 days culture.
Affiliation: School of Biosciences, Sutton Bonington Campus, University of Nottingham, Loughborough, Leicestershire LE12 5RD, United Kingdom.