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A solid state fungal fermentation-based strategy for the hydrolysis of wheat straw.

Pensupa N, Jin M, Kokolski M, Archer DB, Du C - Bioresour. Technol. (2013)

Bottom Line: The addition of yeast extract (0.5% w/v) and minerals significantly improved the cellulase production, to 24.0 ± 1.76 U/g.The hydrolysis of the fermented wheat straw using the fungal culture filtrate or commercial cellulase Ctec2 was performed, resulting in 4.34 and 3.13 g/L glucose respectively.It indicated that the fungal filtrate harvested from the fungal fermentation of wheat straw contained a more suitable enzyme mixture than the commercial cellulase.

View Article: PubMed Central - PubMed

Affiliation: School of Biosciences, Sutton Bonington Campus, University of Nottingham, Loughborough, Leicestershire LE12 5RD, United Kingdom.

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The time courses of glucosamine and enzyme activity produced over the seven days period of the experiment. Autoclaved wheat straw was used as the substrate for the fermentation by A. niger at 28 °C in a static incubator. The liquid to solid ratio of the fermentation was 7.5:1. The experiments were carried out with n = 3 and the error bars indicate standard deviation of each data set.
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f0015: The time courses of glucosamine and enzyme activity produced over the seven days period of the experiment. Autoclaved wheat straw was used as the substrate for the fermentation by A. niger at 28 °C in a static incubator. The liquid to solid ratio of the fermentation was 7.5:1. The experiments were carried out with n = 3 and the error bars indicate standard deviation of each data set.

Mentions: The time course of glucosamine concentration was determined to indicate the biomass concentration of A. niger, as glucosamine was reported to have a positive correlation with the fungal cell growth (Hsieh et al., 2007). As shown in Fig. 3, glucosamine concentration increased fast in the first 3 days, then slowed down. After 7 days of culturing, the glucosamine concentration was still increasing indicating that A. niger still grew after 7 days. The cellulase accumulation reached a maximum at day 3 (9.51 U/g). After that, the cellulase activity declined and levelled off after day 5. This is in agreement with experiments reported by several other researchers (Delabona et al., 2013).


A solid state fungal fermentation-based strategy for the hydrolysis of wheat straw.

Pensupa N, Jin M, Kokolski M, Archer DB, Du C - Bioresour. Technol. (2013)

The time courses of glucosamine and enzyme activity produced over the seven days period of the experiment. Autoclaved wheat straw was used as the substrate for the fermentation by A. niger at 28 °C in a static incubator. The liquid to solid ratio of the fermentation was 7.5:1. The experiments were carried out with n = 3 and the error bars indicate standard deviation of each data set.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3824065&req=5

f0015: The time courses of glucosamine and enzyme activity produced over the seven days period of the experiment. Autoclaved wheat straw was used as the substrate for the fermentation by A. niger at 28 °C in a static incubator. The liquid to solid ratio of the fermentation was 7.5:1. The experiments were carried out with n = 3 and the error bars indicate standard deviation of each data set.
Mentions: The time course of glucosamine concentration was determined to indicate the biomass concentration of A. niger, as glucosamine was reported to have a positive correlation with the fungal cell growth (Hsieh et al., 2007). As shown in Fig. 3, glucosamine concentration increased fast in the first 3 days, then slowed down. After 7 days of culturing, the glucosamine concentration was still increasing indicating that A. niger still grew after 7 days. The cellulase accumulation reached a maximum at day 3 (9.51 U/g). After that, the cellulase activity declined and levelled off after day 5. This is in agreement with experiments reported by several other researchers (Delabona et al., 2013).

Bottom Line: The addition of yeast extract (0.5% w/v) and minerals significantly improved the cellulase production, to 24.0 ± 1.76 U/g.The hydrolysis of the fermented wheat straw using the fungal culture filtrate or commercial cellulase Ctec2 was performed, resulting in 4.34 and 3.13 g/L glucose respectively.It indicated that the fungal filtrate harvested from the fungal fermentation of wheat straw contained a more suitable enzyme mixture than the commercial cellulase.

View Article: PubMed Central - PubMed

Affiliation: School of Biosciences, Sutton Bonington Campus, University of Nottingham, Loughborough, Leicestershire LE12 5RD, United Kingdom.

Show MeSH