A solid state fungal fermentation-based strategy for the hydrolysis of wheat straw.
Bottom Line: The addition of yeast extract (0.5% w/v) and minerals significantly improved the cellulase production, to 24.0 ± 1.76 U/g.The hydrolysis of the fermented wheat straw using the fungal culture filtrate or commercial cellulase Ctec2 was performed, resulting in 4.34 and 3.13 g/L glucose respectively.It indicated that the fungal filtrate harvested from the fungal fermentation of wheat straw contained a more suitable enzyme mixture than the commercial cellulase.
Affiliation: School of Biosciences, Sutton Bonington Campus, University of Nottingham, Loughborough, Leicestershire LE12 5RD, United Kingdom.Show MeSH
Mentions: The time course of glucosamine concentration was determined to indicate the biomass concentration of A. niger, as glucosamine was reported to have a positive correlation with the fungal cell growth (Hsieh et al., 2007). As shown in Fig. 3, glucosamine concentration increased fast in the first 3 days, then slowed down. After 7 days of culturing, the glucosamine concentration was still increasing indicating that A. niger still grew after 7 days. The cellulase accumulation reached a maximum at day 3 (9.51 U/g). After that, the cellulase activity declined and levelled off after day 5. This is in agreement with experiments reported by several other researchers (Delabona et al., 2013).
Affiliation: School of Biosciences, Sutton Bonington Campus, University of Nottingham, Loughborough, Leicestershire LE12 5RD, United Kingdom.