A solid state fungal fermentation-based strategy for the hydrolysis of wheat straw.
Bottom Line: The addition of yeast extract (0.5% w/v) and minerals significantly improved the cellulase production, to 24.0 ± 1.76 U/g.The hydrolysis of the fermented wheat straw using the fungal culture filtrate or commercial cellulase Ctec2 was performed, resulting in 4.34 and 3.13 g/L glucose respectively.It indicated that the fungal filtrate harvested from the fungal fermentation of wheat straw contained a more suitable enzyme mixture than the commercial cellulase.
Affiliation: School of Biosciences, Sutton Bonington Campus, University of Nottingham, Loughborough, Leicestershire LE12 5RD, United Kingdom.Show MeSH
Mentions: The cellulase accumulations from day 3 were analyzed. The highest cellulase activity was around 5.57 U/g obtained in the SSF with a water to wheat straw ratio of 7.5:1 (Fig. 2). This has been statically analyzed by two tails t-test with α = 0.05 and n = 5; the statistics shows that the 7.5:1 case is significantly different from the others. As SSF with 7.5:1 water to wheat straw ratio also resulted in the highest enzyme activity at day 1 (data not shown), this condition was selected for the following experiments.
Affiliation: School of Biosciences, Sutton Bonington Campus, University of Nottingham, Loughborough, Leicestershire LE12 5RD, United Kingdom.