Limits...
A solid state fungal fermentation-based strategy for the hydrolysis of wheat straw.

Pensupa N, Jin M, Kokolski M, Archer DB, Du C - Bioresour. Technol. (2013)

Bottom Line: The addition of yeast extract (0.5% w/v) and minerals significantly improved the cellulase production, to 24.0 ± 1.76 U/g.The hydrolysis of the fermented wheat straw using the fungal culture filtrate or commercial cellulase Ctec2 was performed, resulting in 4.34 and 3.13 g/L glucose respectively.It indicated that the fungal filtrate harvested from the fungal fermentation of wheat straw contained a more suitable enzyme mixture than the commercial cellulase.

View Article: PubMed Central - PubMed

Affiliation: School of Biosciences, Sutton Bonington Campus, University of Nottingham, Loughborough, Leicestershire LE12 5RD, United Kingdom.

Show MeSH
The impact of moisture content on cellulase production. Autoclaved wheat straw was used as the substrate for the fermentation by A. niger at 28 °C in a static incubator. The experiments were carried out with n = 3 and the error bars indicate standard deviation of each data set.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3824065&req=5

f0010: The impact of moisture content on cellulase production. Autoclaved wheat straw was used as the substrate for the fermentation by A. niger at 28 °C in a static incubator. The experiments were carried out with n = 3 and the error bars indicate standard deviation of each data set.

Mentions: The cellulase accumulations from day 3 were analyzed. The highest cellulase activity was around 5.57 U/g obtained in the SSF with a water to wheat straw ratio of 7.5:1 (Fig. 2). This has been statically analyzed by two tails t-test with α = 0.05 and n = 5; the statistics shows that the 7.5:1 case is significantly different from the others. As SSF with 7.5:1 water to wheat straw ratio also resulted in the highest enzyme activity at day 1 (data not shown), this condition was selected for the following experiments.


A solid state fungal fermentation-based strategy for the hydrolysis of wheat straw.

Pensupa N, Jin M, Kokolski M, Archer DB, Du C - Bioresour. Technol. (2013)

The impact of moisture content on cellulase production. Autoclaved wheat straw was used as the substrate for the fermentation by A. niger at 28 °C in a static incubator. The experiments were carried out with n = 3 and the error bars indicate standard deviation of each data set.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3824065&req=5

f0010: The impact of moisture content on cellulase production. Autoclaved wheat straw was used as the substrate for the fermentation by A. niger at 28 °C in a static incubator. The experiments were carried out with n = 3 and the error bars indicate standard deviation of each data set.
Mentions: The cellulase accumulations from day 3 were analyzed. The highest cellulase activity was around 5.57 U/g obtained in the SSF with a water to wheat straw ratio of 7.5:1 (Fig. 2). This has been statically analyzed by two tails t-test with α = 0.05 and n = 5; the statistics shows that the 7.5:1 case is significantly different from the others. As SSF with 7.5:1 water to wheat straw ratio also resulted in the highest enzyme activity at day 1 (data not shown), this condition was selected for the following experiments.

Bottom Line: The addition of yeast extract (0.5% w/v) and minerals significantly improved the cellulase production, to 24.0 ± 1.76 U/g.The hydrolysis of the fermented wheat straw using the fungal culture filtrate or commercial cellulase Ctec2 was performed, resulting in 4.34 and 3.13 g/L glucose respectively.It indicated that the fungal filtrate harvested from the fungal fermentation of wheat straw contained a more suitable enzyme mixture than the commercial cellulase.

View Article: PubMed Central - PubMed

Affiliation: School of Biosciences, Sutton Bonington Campus, University of Nottingham, Loughborough, Leicestershire LE12 5RD, United Kingdom.

Show MeSH