Planarian MBD2/3 is required for adult stem cell pluripotency independently of DNA methylation.
Bottom Line: Here we show the planarian methyl-CpG Binding Domain 2/3 (mbd2/3) gene is required for pASC differentiation during regeneration and tissue homeostasis.The genome does not have detectable levels of 5-methylcytosine (5(m)C) and we find no role for a potential DNA methylase.We conclude that MBD proteins may have had an ancient role in broadly controlling animal stem cell pluripotency, but that DNA methylation is not involved in planarian stem cell differentiation.
Affiliation: Department of Zoology, Tinbergen Building, South Parks Road, University of Oxford, Oxford OX1 3PS, United Kingdom.Show MeSH
Mentions: We observed that Smed-NB.21.11e+ cells were produced during regeneration in mbd2/3(RNAi) animals, and accumulated in regeneration blastemas when compared to control animals (Fig. 5A–C). However, the later Smed-AGAT-1+ stem cell progeny were depleted in mbd2/3(RNAi) animals during regeneration (Fig. 5A, B and D. These data correlated with increased transcript levels for Smed-NB.21.11e and decreased transcript levels of Smed-AGAT-1 (Fig. 5E). Also during regeneration we observed a reduction in the expression of Smed-CYP1A1-1, another marker of late pASC progeny (Eisenhoffer et al., 2008) (Fig. 5F).
Affiliation: Department of Zoology, Tinbergen Building, South Parks Road, University of Oxford, Oxford OX1 3PS, United Kingdom.