Planarian MBD2/3 is required for adult stem cell pluripotency independently of DNA methylation.
Bottom Line: Here we show the planarian methyl-CpG Binding Domain 2/3 (mbd2/3) gene is required for pASC differentiation during regeneration and tissue homeostasis.The genome does not have detectable levels of 5-methylcytosine (5(m)C) and we find no role for a potential DNA methylase.We conclude that MBD proteins may have had an ancient role in broadly controlling animal stem cell pluripotency, but that DNA methylation is not involved in planarian stem cell differentiation.
Affiliation: Department of Zoology, Tinbergen Building, South Parks Road, University of Oxford, Oxford OX1 3PS, United Kingdom.Show MeSH
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Mentions: We observed no effect on the distribution, proliferation or relative numbers of pASCs during tissue homeostasis or regeneration in mbd2/3(RNAi) animals relative to their controls (Fig. 4A–F; Supplementary Fig. 3A–C). Even animals that had regressed to tail stumps after 3 weeks of homeostasis had normal Smedwi-2 expression in pASCs and normal levels of mitosis (Fig. 4A and Supplementary Fig. 3A). We subjected mbd2/3(RNAi) animals to a further round of standard RNAi injections and regeneration and also observed no defect in proliferation (Supplementary Fig. 3C).
Affiliation: Department of Zoology, Tinbergen Building, South Parks Road, University of Oxford, Oxford OX1 3PS, United Kingdom.