Planarian MBD2/3 is required for adult stem cell pluripotency independently of DNA methylation.
Bottom Line: Here we show the planarian methyl-CpG Binding Domain 2/3 (mbd2/3) gene is required for pASC differentiation during regeneration and tissue homeostasis.The genome does not have detectable levels of 5-methylcytosine (5(m)C) and we find no role for a potential DNA methylase.We conclude that MBD proteins may have had an ancient role in broadly controlling animal stem cell pluripotency, but that DNA methylation is not involved in planarian stem cell differentiation.
Affiliation: Department of Zoology, Tinbergen Building, South Parks Road, University of Oxford, Oxford OX1 3PS, United Kingdom.Show MeSH
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Mentions: The widespread differentiation defects observed in mbd2/3(RNAi) worms during regeneration were also apparent in worms that were left to undergo normal tissue homeostasis. After 2 weeks of homeostasis (equivalent to 14 days of regeneration) most animals started to show some degree of anterior regression, starting with the anterior tip and anterior lateral margins (Fig. 3A). We found that head regression correlated with a loss of brain ganglia cells (Fig. 3B). At 2 weeks of homeostasis we also observed loss of anterior gut branches, which correlated with the regression of medial tissue between the brain ganglia (Fig. 3C, 9/10 animals and Fig. 3D, 5/5 animals).
Affiliation: Department of Zoology, Tinbergen Building, South Parks Road, University of Oxford, Oxford OX1 3PS, United Kingdom.