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Planarian MBD2/3 is required for adult stem cell pluripotency independently of DNA methylation.

Jaber-Hijazi F, Lo PJ, Mihaylova Y, Foster JM, Benner JS, Tejada Romero B, Chen C, Malla S, Solana J, Ruzov A, Aziz Aboobaker A - Dev. Biol. (2013)

Bottom Line: Here we show the planarian methyl-CpG Binding Domain 2/3 (mbd2/3) gene is required for pASC differentiation during regeneration and tissue homeostasis.The genome does not have detectable levels of 5-methylcytosine (5(m)C) and we find no role for a potential DNA methylase.We conclude that MBD proteins may have had an ancient role in broadly controlling animal stem cell pluripotency, but that DNA methylation is not involved in planarian stem cell differentiation.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, Tinbergen Building, South Parks Road, University of Oxford, Oxford OX1 3PS, United Kingdom.

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Related in: MedlinePlus

mbd2/3(RNAi) Leads to disruption of tissue homeostasis. Most mbd2/3(RNAi) animals start to regress anterior regions by 2 weeks varying from mild to more extreme phenotypes (A). Head regression correlates with loss of cephalic ganglia (B) and the anterior gut branches (C) and (D). At 3 weeks almost all animals have completely lost anterior regions (red arrow-head), although a few display only mild anterior regression (E). By three weeks posterior gut branches have also started to be lost (F)–(H). While gut branches (blue arrows in (G)) and the pharynx (stars in (H) and (J)) are lost, the VNCs (white arrows in (J)) are maintained (F)–(H) and (J). Animals at 4 weeks have regressed further, but are mostly still alive (I). Scale bars 500 µm, except in C and G 200 µm. Panels in G represent regions in white boxes depicted in panel F.
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f0015: mbd2/3(RNAi) Leads to disruption of tissue homeostasis. Most mbd2/3(RNAi) animals start to regress anterior regions by 2 weeks varying from mild to more extreme phenotypes (A). Head regression correlates with loss of cephalic ganglia (B) and the anterior gut branches (C) and (D). At 3 weeks almost all animals have completely lost anterior regions (red arrow-head), although a few display only mild anterior regression (E). By three weeks posterior gut branches have also started to be lost (F)–(H). While gut branches (blue arrows in (G)) and the pharynx (stars in (H) and (J)) are lost, the VNCs (white arrows in (J)) are maintained (F)–(H) and (J). Animals at 4 weeks have regressed further, but are mostly still alive (I). Scale bars 500 µm, except in C and G 200 µm. Panels in G represent regions in white boxes depicted in panel F.

Mentions: The widespread differentiation defects observed in mbd2/3(RNAi) worms during regeneration were also apparent in worms that were left to undergo normal tissue homeostasis. After 2 weeks of homeostasis (equivalent to 14 days of regeneration) most animals started to show some degree of anterior regression, starting with the anterior tip and anterior lateral margins (Fig. 3A). We found that head regression correlated with a loss of brain ganglia cells (Fig. 3B). At 2 weeks of homeostasis we also observed loss of anterior gut branches, which correlated with the regression of medial tissue between the brain ganglia (Fig. 3C, 9/10 animals and Fig. 3D, 5/5 animals).


Planarian MBD2/3 is required for adult stem cell pluripotency independently of DNA methylation.

Jaber-Hijazi F, Lo PJ, Mihaylova Y, Foster JM, Benner JS, Tejada Romero B, Chen C, Malla S, Solana J, Ruzov A, Aziz Aboobaker A - Dev. Biol. (2013)

mbd2/3(RNAi) Leads to disruption of tissue homeostasis. Most mbd2/3(RNAi) animals start to regress anterior regions by 2 weeks varying from mild to more extreme phenotypes (A). Head regression correlates with loss of cephalic ganglia (B) and the anterior gut branches (C) and (D). At 3 weeks almost all animals have completely lost anterior regions (red arrow-head), although a few display only mild anterior regression (E). By three weeks posterior gut branches have also started to be lost (F)–(H). While gut branches (blue arrows in (G)) and the pharynx (stars in (H) and (J)) are lost, the VNCs (white arrows in (J)) are maintained (F)–(H) and (J). Animals at 4 weeks have regressed further, but are mostly still alive (I). Scale bars 500 µm, except in C and G 200 µm. Panels in G represent regions in white boxes depicted in panel F.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC3824064&req=5

f0015: mbd2/3(RNAi) Leads to disruption of tissue homeostasis. Most mbd2/3(RNAi) animals start to regress anterior regions by 2 weeks varying from mild to more extreme phenotypes (A). Head regression correlates with loss of cephalic ganglia (B) and the anterior gut branches (C) and (D). At 3 weeks almost all animals have completely lost anterior regions (red arrow-head), although a few display only mild anterior regression (E). By three weeks posterior gut branches have also started to be lost (F)–(H). While gut branches (blue arrows in (G)) and the pharynx (stars in (H) and (J)) are lost, the VNCs (white arrows in (J)) are maintained (F)–(H) and (J). Animals at 4 weeks have regressed further, but are mostly still alive (I). Scale bars 500 µm, except in C and G 200 µm. Panels in G represent regions in white boxes depicted in panel F.
Mentions: The widespread differentiation defects observed in mbd2/3(RNAi) worms during regeneration were also apparent in worms that were left to undergo normal tissue homeostasis. After 2 weeks of homeostasis (equivalent to 14 days of regeneration) most animals started to show some degree of anterior regression, starting with the anterior tip and anterior lateral margins (Fig. 3A). We found that head regression correlated with a loss of brain ganglia cells (Fig. 3B). At 2 weeks of homeostasis we also observed loss of anterior gut branches, which correlated with the regression of medial tissue between the brain ganglia (Fig. 3C, 9/10 animals and Fig. 3D, 5/5 animals).

Bottom Line: Here we show the planarian methyl-CpG Binding Domain 2/3 (mbd2/3) gene is required for pASC differentiation during regeneration and tissue homeostasis.The genome does not have detectable levels of 5-methylcytosine (5(m)C) and we find no role for a potential DNA methylase.We conclude that MBD proteins may have had an ancient role in broadly controlling animal stem cell pluripotency, but that DNA methylation is not involved in planarian stem cell differentiation.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, Tinbergen Building, South Parks Road, University of Oxford, Oxford OX1 3PS, United Kingdom.

Show MeSH
Related in: MedlinePlus