Limits...
68Ga-triacetylfusarinine C and 68Ga-ferrioxamine E for Aspergillus infection imaging: uptake specificity in various microorganisms.

Petrik M, Haas H, Laverman P, Schrettl M, Franssen GM, Blatzer M, Decristoforo C - Mol Imaging Biol (2014)

Bottom Line: Here, we report on the comparison of specificity towards different microorganisms and human lung cancer cells (H1299).The in vivo imaging was studied in fungal and bacterial rat infection and inflammation models. (68)Ga-TAFC and (68)Ga-FOXE showed rapid uptake in A. fumigatus cultures, significantly lower in other fungal species and almost no uptake in other microorganisms and H1299 cells, except for (68)Ga-FOXE in Staphylococcus aureus. (68)Ga-TAFC and (68)Ga-FOXE revealed rapid uptake in the lungs of A. fumigatus-infected rats, low accumulation in sterile inflammation and no uptake in bacterial abscess.We have shown that (68)Ga-FOXE and (68)Ga-TAFC have high uptake in A. fumigatus both in vitro and in vivo. (68)Ga-TAFC showed higher specificity, while (68)Ga-FOXE showed higher sensitivity.

View Article: PubMed Central - PubMed

Affiliation: Clinical Department of Nuclear Medicine, Innsbruck Medical University, Anichstrasse 35, 6020, Innsbruck, Austria, milospetrik@seznam.cz.

ABSTRACT

Purpose: (68)Ga-triacetylfusarinine C ((68)Ga-TAFC) and (68)Ga-ferrioxamine E ((68)Ga-FOXE) showed excellent targeting properties in Aspergillus fumigatus rat infection model. Here, we report on the comparison of specificity towards different microorganisms and human lung cancer cells (H1299).

Procedures: The in vitro uptake of (68)Ga-TAFC and (68)Ga-FOXE was studied in various fungal, bacterial and yeast cultures as well as in H1299 cells. The in vivo imaging was studied in fungal and bacterial rat infection and inflammation models.

Results: (68)Ga-TAFC and (68)Ga-FOXE showed rapid uptake in A. fumigatus cultures, significantly lower in other fungal species and almost no uptake in other microorganisms and H1299 cells, except for (68)Ga-FOXE in Staphylococcus aureus. (68)Ga-TAFC and (68)Ga-FOXE revealed rapid uptake in the lungs of A. fumigatus-infected rats, low accumulation in sterile inflammation and no uptake in bacterial abscess.

Conclusions: We have shown that (68)Ga-FOXE and (68)Ga-TAFC have high uptake in A. fumigatus both in vitro and in vivo. (68)Ga-TAFC showed higher specificity, while (68)Ga-FOXE showed higher sensitivity.

Show MeSH

Related in: MedlinePlus

In vivo imaging of the A. fumigatus rat infection model (left-hand side images) and non-infected (right-hand side images) animals using a68Ga-TAFC and b68Ga-FOXE, 1 h. i.v. postinjection.
© Copyright Policy - OpenAccess
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3823598&req=5

Fig3: In vivo imaging of the A. fumigatus rat infection model (left-hand side images) and non-infected (right-hand side images) animals using a68Ga-TAFC and b68Ga-FOXE, 1 h. i.v. postinjection.

Mentions: In vivo PET imaging in the A. fumigatus rat infection model showed rapid focal accumulation of 68Ga-siderophores in the lungs. Whereas no in vivo uptake in the lung region was detected in non-infected animals, and the only visible organs were the kidneys and bladder (see Fig. 3).Fig. 3


68Ga-triacetylfusarinine C and 68Ga-ferrioxamine E for Aspergillus infection imaging: uptake specificity in various microorganisms.

Petrik M, Haas H, Laverman P, Schrettl M, Franssen GM, Blatzer M, Decristoforo C - Mol Imaging Biol (2014)

In vivo imaging of the A. fumigatus rat infection model (left-hand side images) and non-infected (right-hand side images) animals using a68Ga-TAFC and b68Ga-FOXE, 1 h. i.v. postinjection.
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3823598&req=5

Fig3: In vivo imaging of the A. fumigatus rat infection model (left-hand side images) and non-infected (right-hand side images) animals using a68Ga-TAFC and b68Ga-FOXE, 1 h. i.v. postinjection.
Mentions: In vivo PET imaging in the A. fumigatus rat infection model showed rapid focal accumulation of 68Ga-siderophores in the lungs. Whereas no in vivo uptake in the lung region was detected in non-infected animals, and the only visible organs were the kidneys and bladder (see Fig. 3).Fig. 3

Bottom Line: Here, we report on the comparison of specificity towards different microorganisms and human lung cancer cells (H1299).The in vivo imaging was studied in fungal and bacterial rat infection and inflammation models. (68)Ga-TAFC and (68)Ga-FOXE showed rapid uptake in A. fumigatus cultures, significantly lower in other fungal species and almost no uptake in other microorganisms and H1299 cells, except for (68)Ga-FOXE in Staphylococcus aureus. (68)Ga-TAFC and (68)Ga-FOXE revealed rapid uptake in the lungs of A. fumigatus-infected rats, low accumulation in sterile inflammation and no uptake in bacterial abscess.We have shown that (68)Ga-FOXE and (68)Ga-TAFC have high uptake in A. fumigatus both in vitro and in vivo. (68)Ga-TAFC showed higher specificity, while (68)Ga-FOXE showed higher sensitivity.

View Article: PubMed Central - PubMed

Affiliation: Clinical Department of Nuclear Medicine, Innsbruck Medical University, Anichstrasse 35, 6020, Innsbruck, Austria, milospetrik@seznam.cz.

ABSTRACT

Purpose: (68)Ga-triacetylfusarinine C ((68)Ga-TAFC) and (68)Ga-ferrioxamine E ((68)Ga-FOXE) showed excellent targeting properties in Aspergillus fumigatus rat infection model. Here, we report on the comparison of specificity towards different microorganisms and human lung cancer cells (H1299).

Procedures: The in vitro uptake of (68)Ga-TAFC and (68)Ga-FOXE was studied in various fungal, bacterial and yeast cultures as well as in H1299 cells. The in vivo imaging was studied in fungal and bacterial rat infection and inflammation models.

Results: (68)Ga-TAFC and (68)Ga-FOXE showed rapid uptake in A. fumigatus cultures, significantly lower in other fungal species and almost no uptake in other microorganisms and H1299 cells, except for (68)Ga-FOXE in Staphylococcus aureus. (68)Ga-TAFC and (68)Ga-FOXE revealed rapid uptake in the lungs of A. fumigatus-infected rats, low accumulation in sterile inflammation and no uptake in bacterial abscess.

Conclusions: We have shown that (68)Ga-FOXE and (68)Ga-TAFC have high uptake in A. fumigatus both in vitro and in vivo. (68)Ga-TAFC showed higher specificity, while (68)Ga-FOXE showed higher sensitivity.

Show MeSH
Related in: MedlinePlus