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68Ga-triacetylfusarinine C and 68Ga-ferrioxamine E for Aspergillus infection imaging: uptake specificity in various microorganisms.

Petrik M, Haas H, Laverman P, Schrettl M, Franssen GM, Blatzer M, Decristoforo C - Mol Imaging Biol (2014)

Bottom Line: Here, we report on the comparison of specificity towards different microorganisms and human lung cancer cells (H1299).The in vivo imaging was studied in fungal and bacterial rat infection and inflammation models. (68)Ga-TAFC and (68)Ga-FOXE showed rapid uptake in A. fumigatus cultures, significantly lower in other fungal species and almost no uptake in other microorganisms and H1299 cells, except for (68)Ga-FOXE in Staphylococcus aureus. (68)Ga-TAFC and (68)Ga-FOXE revealed rapid uptake in the lungs of A. fumigatus-infected rats, low accumulation in sterile inflammation and no uptake in bacterial abscess.We have shown that (68)Ga-FOXE and (68)Ga-TAFC have high uptake in A. fumigatus both in vitro and in vivo. (68)Ga-TAFC showed higher specificity, while (68)Ga-FOXE showed higher sensitivity.

View Article: PubMed Central - PubMed

Affiliation: Clinical Department of Nuclear Medicine, Innsbruck Medical University, Anichstrasse 35, 6020, Innsbruck, Austria, milospetrik@seznam.cz.

ABSTRACT

Purpose: (68)Ga-triacetylfusarinine C ((68)Ga-TAFC) and (68)Ga-ferrioxamine E ((68)Ga-FOXE) showed excellent targeting properties in Aspergillus fumigatus rat infection model. Here, we report on the comparison of specificity towards different microorganisms and human lung cancer cells (H1299).

Procedures: The in vitro uptake of (68)Ga-TAFC and (68)Ga-FOXE was studied in various fungal, bacterial and yeast cultures as well as in H1299 cells. The in vivo imaging was studied in fungal and bacterial rat infection and inflammation models.

Results: (68)Ga-TAFC and (68)Ga-FOXE showed rapid uptake in A. fumigatus cultures, significantly lower in other fungal species and almost no uptake in other microorganisms and H1299 cells, except for (68)Ga-FOXE in Staphylococcus aureus. (68)Ga-TAFC and (68)Ga-FOXE revealed rapid uptake in the lungs of A. fumigatus-infected rats, low accumulation in sterile inflammation and no uptake in bacterial abscess.

Conclusions: We have shown that (68)Ga-FOXE and (68)Ga-TAFC have high uptake in A. fumigatus both in vitro and in vivo. (68)Ga-TAFC showed higher specificity, while (68)Ga-FOXE showed higher sensitivity.

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Related in: MedlinePlus

Siderophore utilisation assay demonstrating that growth of K. pneumoniae is in contrast to that of A. fumigatus inhibited by ligand-free TAFC.
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Fig2: Siderophore utilisation assay demonstrating that growth of K. pneumoniae is in contrast to that of A. fumigatus inhibited by ligand-free TAFC.

Mentions: To exemplary confirm the ability or inability to take up TAFC, we developed a siderophore utilisation growth assay (Fig. 2). In this agar diffusion assay, the analysed species (107 conidia of A. fumigatus or 0.2 ml of K. pneumoniae preculture, respectively) was poured in 5 ml top agar (iron-limiting AMM medium + 0.7 % agar) on agar plates (iron-limiting AMM medium). Subsequently, 80 μl of 0.6 mM ligand-free TAFC solution was inoculated into a hole (5 mm diameter) punched into the middle of the plate. The plates were then incubated at 37 °C for 30 h. The ligand-free siderophore diffuses into the growth medium and chelates the present iron with the highest TAFC concentration in the vicinity of the hole. The growth of microorganisms unable to take up TAFC-iron is inhibited by high TAFC concentrations in this assay as TAFC iron is here the only iron source present.


68Ga-triacetylfusarinine C and 68Ga-ferrioxamine E for Aspergillus infection imaging: uptake specificity in various microorganisms.

Petrik M, Haas H, Laverman P, Schrettl M, Franssen GM, Blatzer M, Decristoforo C - Mol Imaging Biol (2014)

Siderophore utilisation assay demonstrating that growth of K. pneumoniae is in contrast to that of A. fumigatus inhibited by ligand-free TAFC.
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3823598&req=5

Fig2: Siderophore utilisation assay demonstrating that growth of K. pneumoniae is in contrast to that of A. fumigatus inhibited by ligand-free TAFC.
Mentions: To exemplary confirm the ability or inability to take up TAFC, we developed a siderophore utilisation growth assay (Fig. 2). In this agar diffusion assay, the analysed species (107 conidia of A. fumigatus or 0.2 ml of K. pneumoniae preculture, respectively) was poured in 5 ml top agar (iron-limiting AMM medium + 0.7 % agar) on agar plates (iron-limiting AMM medium). Subsequently, 80 μl of 0.6 mM ligand-free TAFC solution was inoculated into a hole (5 mm diameter) punched into the middle of the plate. The plates were then incubated at 37 °C for 30 h. The ligand-free siderophore diffuses into the growth medium and chelates the present iron with the highest TAFC concentration in the vicinity of the hole. The growth of microorganisms unable to take up TAFC-iron is inhibited by high TAFC concentrations in this assay as TAFC iron is here the only iron source present.

Bottom Line: Here, we report on the comparison of specificity towards different microorganisms and human lung cancer cells (H1299).The in vivo imaging was studied in fungal and bacterial rat infection and inflammation models. (68)Ga-TAFC and (68)Ga-FOXE showed rapid uptake in A. fumigatus cultures, significantly lower in other fungal species and almost no uptake in other microorganisms and H1299 cells, except for (68)Ga-FOXE in Staphylococcus aureus. (68)Ga-TAFC and (68)Ga-FOXE revealed rapid uptake in the lungs of A. fumigatus-infected rats, low accumulation in sterile inflammation and no uptake in bacterial abscess.We have shown that (68)Ga-FOXE and (68)Ga-TAFC have high uptake in A. fumigatus both in vitro and in vivo. (68)Ga-TAFC showed higher specificity, while (68)Ga-FOXE showed higher sensitivity.

View Article: PubMed Central - PubMed

Affiliation: Clinical Department of Nuclear Medicine, Innsbruck Medical University, Anichstrasse 35, 6020, Innsbruck, Austria, milospetrik@seznam.cz.

ABSTRACT

Purpose: (68)Ga-triacetylfusarinine C ((68)Ga-TAFC) and (68)Ga-ferrioxamine E ((68)Ga-FOXE) showed excellent targeting properties in Aspergillus fumigatus rat infection model. Here, we report on the comparison of specificity towards different microorganisms and human lung cancer cells (H1299).

Procedures: The in vitro uptake of (68)Ga-TAFC and (68)Ga-FOXE was studied in various fungal, bacterial and yeast cultures as well as in H1299 cells. The in vivo imaging was studied in fungal and bacterial rat infection and inflammation models.

Results: (68)Ga-TAFC and (68)Ga-FOXE showed rapid uptake in A. fumigatus cultures, significantly lower in other fungal species and almost no uptake in other microorganisms and H1299 cells, except for (68)Ga-FOXE in Staphylococcus aureus. (68)Ga-TAFC and (68)Ga-FOXE revealed rapid uptake in the lungs of A. fumigatus-infected rats, low accumulation in sterile inflammation and no uptake in bacterial abscess.

Conclusions: We have shown that (68)Ga-FOXE and (68)Ga-TAFC have high uptake in A. fumigatus both in vitro and in vivo. (68)Ga-TAFC showed higher specificity, while (68)Ga-FOXE showed higher sensitivity.

Show MeSH
Related in: MedlinePlus