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Enhancing the laccase production and laccase gene expression in the white-rot fungus Trametes velutina 5930 with great potential for biotechnological applications by different metal ions and aromatic compounds.

Yang Y, Wei F, Zhuo R, Fan F, Liu H, Zhang C, Ma L, Jiang M, Zhang X - PLoS ONE (2013)

Bottom Line: Some aromatic compounds structurally related to lignin, such as tannic acid, syringic acid, cinnamic acid, gallic acid and guaiacol, could also enhance the level of laccase activity and laccase gene transcription.We also found that there existed a positive synergistic effect of aromatic compound and metal ion on the laccase production and laccase gene transcription in Trametes velutina 5930.Taken together, our study may contribute to the improvement of laccase productivity by Trametes velutina 5930.

View Article: PubMed Central - PubMed

Affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China ; Key Laboratory of Oil Crops Biology of Ministry of Agriculture in China, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan, China.

ABSTRACT
Laccase is useful for various biotechnological and industrial applications. The white-rot fungus Trametes velutina 5930 and its laccase, isolated from the Shennongjia Nature Reserve in China by our laboratory, has great potential for practical application in environmental biotechnology. However, the original level of laccase produced by Trametes velutina 5930 was relatively low in the absence of any inducer. Therefore, in order to enhance the laccase production by Trametes velutina 5930 and make better use of this fungus in the field of environmental biotechnology, the regulation of laccase production and laccase gene expression in Trametes velutina 5930 were investigated in this study. Different metal ions such as Cu(2+) and Fe(2+) could stimulate the laccase synthesis and laccase gene transcription in Trametes velutina 5930. Some aromatic compounds structurally related to lignin, such as tannic acid, syringic acid, cinnamic acid, gallic acid and guaiacol, could also enhance the level of laccase activity and laccase gene transcription. We also found that there existed a positive synergistic effect of aromatic compound and metal ion on the laccase production and laccase gene transcription in Trametes velutina 5930. Taken together, our study may contribute to the improvement of laccase productivity by Trametes velutina 5930.

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Related in: MedlinePlus

Tolerance of Trametes velutina 5930 to the aromatic compounds including tannic acid, cinnamic acid, syringic acid, gallic acid and guaiacol.The various concentrations of aromatic compounds were added to 100
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getmorefigures.php?uid=PMC3823595&req=5

pone-0079307-g004: Tolerance of Trametes velutina 5930 to the aromatic compounds including tannic acid, cinnamic acid, syringic acid, gallic acid and guaiacol.The various concentrations of aromatic compounds were added to 100

Mentions: In order to determine the toxicity of different aromatic compounds to the growth of Trametes velutina 5930, the various concentrations of aromatic compounds were added to the medium at the time of inoculation, then the mycelial dry weight of cultures after 7 days of growth were measured. As shown in Fig. 4, the aromatic compounds tested in this study did not inhibit the growth of Trametes velutina 5930 in submerged cultures.


Enhancing the laccase production and laccase gene expression in the white-rot fungus Trametes velutina 5930 with great potential for biotechnological applications by different metal ions and aromatic compounds.

Yang Y, Wei F, Zhuo R, Fan F, Liu H, Zhang C, Ma L, Jiang M, Zhang X - PLoS ONE (2013)

Tolerance of Trametes velutina 5930 to the aromatic compounds including tannic acid, cinnamic acid, syringic acid, gallic acid and guaiacol.The various concentrations of aromatic compounds were added to 100
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3823595&req=5

pone-0079307-g004: Tolerance of Trametes velutina 5930 to the aromatic compounds including tannic acid, cinnamic acid, syringic acid, gallic acid and guaiacol.The various concentrations of aromatic compounds were added to 100
Mentions: In order to determine the toxicity of different aromatic compounds to the growth of Trametes velutina 5930, the various concentrations of aromatic compounds were added to the medium at the time of inoculation, then the mycelial dry weight of cultures after 7 days of growth were measured. As shown in Fig. 4, the aromatic compounds tested in this study did not inhibit the growth of Trametes velutina 5930 in submerged cultures.

Bottom Line: Some aromatic compounds structurally related to lignin, such as tannic acid, syringic acid, cinnamic acid, gallic acid and guaiacol, could also enhance the level of laccase activity and laccase gene transcription.We also found that there existed a positive synergistic effect of aromatic compound and metal ion on the laccase production and laccase gene transcription in Trametes velutina 5930.Taken together, our study may contribute to the improvement of laccase productivity by Trametes velutina 5930.

View Article: PubMed Central - PubMed

Affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China ; Key Laboratory of Oil Crops Biology of Ministry of Agriculture in China, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan, China.

ABSTRACT
Laccase is useful for various biotechnological and industrial applications. The white-rot fungus Trametes velutina 5930 and its laccase, isolated from the Shennongjia Nature Reserve in China by our laboratory, has great potential for practical application in environmental biotechnology. However, the original level of laccase produced by Trametes velutina 5930 was relatively low in the absence of any inducer. Therefore, in order to enhance the laccase production by Trametes velutina 5930 and make better use of this fungus in the field of environmental biotechnology, the regulation of laccase production and laccase gene expression in Trametes velutina 5930 were investigated in this study. Different metal ions such as Cu(2+) and Fe(2+) could stimulate the laccase synthesis and laccase gene transcription in Trametes velutina 5930. Some aromatic compounds structurally related to lignin, such as tannic acid, syringic acid, cinnamic acid, gallic acid and guaiacol, could also enhance the level of laccase activity and laccase gene transcription. We also found that there existed a positive synergistic effect of aromatic compound and metal ion on the laccase production and laccase gene transcription in Trametes velutina 5930. Taken together, our study may contribute to the improvement of laccase productivity by Trametes velutina 5930.

Show MeSH
Related in: MedlinePlus