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Preparation, drug releasing property and pharmacodynamics of soy isoflavone-loaded chitosan microspheres.

Du Z, Dou X, Huang C, Gao J, Hu L, Zhu J, Qian Y, Dou M, Fan C - PLoS ONE (2013)

Bottom Line: In this paper, soybean isoflavone sustained-release microsphere from chitosan and sodium alginate was prepared successfully.In real gastrointestinal conditions, compared with the non-sustained release group, the release rate of SIF slowed down and the reaction time was prolonged.Compared with the Non-sustained release (NSR) group mice, oral SIF/CHI microsphere treated mice were better in the Morris Water Maze Test (MWMT), the MDA level in the both plasma and brain of the sustained release (SR) group mice decreased, and SOD content was remarkably improved.

View Article: PubMed Central - PubMed

Affiliation: College of Life Science, Zhejiang Chinese Medical University, Hangzhou, PR China.

ABSTRACT
Soybean isoflavone (SIF) has anti-aging properties and many other biological functions; however, SIF is difficult to reach higher blood concentration due to its rapid metabolism. Therefore, it is of great value to design and produce a sustained-release formulation that is able to maintain a stable level of plasma concentrations. In this paper, soybean isoflavone sustained-release microsphere from chitosan and sodium alginate was prepared successfully. The important factors that determined the quality of the microspheres were the sodium alginate concentration in solution B, the ratio of soybean isoflavone to chitosan and the mixing speed. The relative yield, encapsulation efficiency and drug loading capability of SIF were much higher than the existing commercial formulations. In real gastrointestinal conditions, compared with the non-sustained release group, the release rate of SIF slowed down and the reaction time was prolonged. Animal experiments showed that sustained-release microspheres intensified the anti-aging potentials of SIF. Compared with the Non-sustained release (NSR) group mice, oral SIF/CHI microsphere treated mice were better in the Morris Water Maze Test (MWMT), the MDA level in the both plasma and brain of the sustained release (SR) group mice decreased, and SOD content was remarkably improved.

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Determination of In Vitro Release (R %) of SIF/CHI Microspheres containing different chitosan concentration.
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pone-0079698-g003: Determination of In Vitro Release (R %) of SIF/CHI Microspheres containing different chitosan concentration.

Mentions: An improved UV Spectrophotometry method was used for determining the concentration of SIF encapsulated in chitosan microspheres. The UV-visible spectrum recorded for a solution of standard sample and dry soy extract solution showed an intense absorption band with a maximum wavelength at 260 nm indicative of SIF (Figure 2A). A similar absorption band was present in the UV-visible spectrum of genistein. Figure 2B showed the standard curve between the absorbance and concentration, which had a linear relationship when the sample concentration was in the range of 0-12.5μg/mL (R = 0.9999). According to the standard curve, Tables 1-3 showed the RY (%), DLC (%), and EE (%) of different batches of microspheres prepared under different conditions. As seen in Figure 3, the concentration of chitosan had a significant impact on the rate at which SIF was released from the microspheres. Among the three concentrations assayed, microspheres consisting of chitosan at 5 mg/mL were shown to be the best for the sustained release of SIF. In comparison, at chitosan concentration of 2 mg/mL, microspheres exhibited the fastest release of SIF among the three at the early stage of the experiment, whereas at 10 mg/mL, an acceleration was observed between T=2 h and T=4 h. As a result, the best microsphere was prepared under the following optimized conditions: solution B containing 1.5% (w/v) sodium alginate, 0.03 mg/L soy-isoflavone, 5 mg/ml chitosan, when mixing speed achieved 400 rpm. All of the SIF/CHI microspheres used in the following experiments were prepared according to the optimized conditions.


Preparation, drug releasing property and pharmacodynamics of soy isoflavone-loaded chitosan microspheres.

Du Z, Dou X, Huang C, Gao J, Hu L, Zhu J, Qian Y, Dou M, Fan C - PLoS ONE (2013)

Determination of In Vitro Release (R %) of SIF/CHI Microspheres containing different chitosan concentration.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3823575&req=5

pone-0079698-g003: Determination of In Vitro Release (R %) of SIF/CHI Microspheres containing different chitosan concentration.
Mentions: An improved UV Spectrophotometry method was used for determining the concentration of SIF encapsulated in chitosan microspheres. The UV-visible spectrum recorded for a solution of standard sample and dry soy extract solution showed an intense absorption band with a maximum wavelength at 260 nm indicative of SIF (Figure 2A). A similar absorption band was present in the UV-visible spectrum of genistein. Figure 2B showed the standard curve between the absorbance and concentration, which had a linear relationship when the sample concentration was in the range of 0-12.5μg/mL (R = 0.9999). According to the standard curve, Tables 1-3 showed the RY (%), DLC (%), and EE (%) of different batches of microspheres prepared under different conditions. As seen in Figure 3, the concentration of chitosan had a significant impact on the rate at which SIF was released from the microspheres. Among the three concentrations assayed, microspheres consisting of chitosan at 5 mg/mL were shown to be the best for the sustained release of SIF. In comparison, at chitosan concentration of 2 mg/mL, microspheres exhibited the fastest release of SIF among the three at the early stage of the experiment, whereas at 10 mg/mL, an acceleration was observed between T=2 h and T=4 h. As a result, the best microsphere was prepared under the following optimized conditions: solution B containing 1.5% (w/v) sodium alginate, 0.03 mg/L soy-isoflavone, 5 mg/ml chitosan, when mixing speed achieved 400 rpm. All of the SIF/CHI microspheres used in the following experiments were prepared according to the optimized conditions.

Bottom Line: In this paper, soybean isoflavone sustained-release microsphere from chitosan and sodium alginate was prepared successfully.In real gastrointestinal conditions, compared with the non-sustained release group, the release rate of SIF slowed down and the reaction time was prolonged.Compared with the Non-sustained release (NSR) group mice, oral SIF/CHI microsphere treated mice were better in the Morris Water Maze Test (MWMT), the MDA level in the both plasma and brain of the sustained release (SR) group mice decreased, and SOD content was remarkably improved.

View Article: PubMed Central - PubMed

Affiliation: College of Life Science, Zhejiang Chinese Medical University, Hangzhou, PR China.

ABSTRACT
Soybean isoflavone (SIF) has anti-aging properties and many other biological functions; however, SIF is difficult to reach higher blood concentration due to its rapid metabolism. Therefore, it is of great value to design and produce a sustained-release formulation that is able to maintain a stable level of plasma concentrations. In this paper, soybean isoflavone sustained-release microsphere from chitosan and sodium alginate was prepared successfully. The important factors that determined the quality of the microspheres were the sodium alginate concentration in solution B, the ratio of soybean isoflavone to chitosan and the mixing speed. The relative yield, encapsulation efficiency and drug loading capability of SIF were much higher than the existing commercial formulations. In real gastrointestinal conditions, compared with the non-sustained release group, the release rate of SIF slowed down and the reaction time was prolonged. Animal experiments showed that sustained-release microspheres intensified the anti-aging potentials of SIF. Compared with the Non-sustained release (NSR) group mice, oral SIF/CHI microsphere treated mice were better in the Morris Water Maze Test (MWMT), the MDA level in the both plasma and brain of the sustained release (SR) group mice decreased, and SOD content was remarkably improved.

Show MeSH