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Regeneration of infarcted myocardium with resveratrol-modified cardiac stem cells.

Gorbunov N, Petrovski G, Gurusamy N, Ray D, Kim do H, Das DK - J. Cell. Mol. Med. (2012)

Bottom Line: Indeed, the vast majority of host-transfused cells do not survive beyond 24-72 hrs.Rats were anaesthetized, hearts opened and the LAD occluded to induce heart attack.One week later, the cardiac reduced environment was confirmed in resveratrol treated rat hearts by the enhanced expression of nuclear factor-E2-related factor-2 (Nrf2) and redox effector factor-1 (Ref-1).

View Article: PubMed Central - PubMed

Affiliation: The Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MD, USA.

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Immunofluorescence assessment of the combined effect of cardiac stem cells on the expression of SDF-1 and myosin in myocardium after 4 months following implantation. (A, B) Projections of SDF-1 in the rat heart specimens from control and LAD-treated groups, respectively. (C, D) Relative intensities of immunofluorescence of SDF-1 shown in (A) and (B), respectively. Stem cells were labelled with EGFP (in green) as indicated in Materials and Methods. Fluorescent signals from SDF-1 and nuclei were collected in the red and blue channels, respectively. Localization of SDF-1 in EGFP-labelled cells is indicated with white arrows. (E, F) Projections of myosin (in red) in the rat heart specimens from control and LAD-treated groups, respectively. (G, H) Relative intensities of immunofluorescence of myosin shown in (E) and (F), respectively.
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fig05: Immunofluorescence assessment of the combined effect of cardiac stem cells on the expression of SDF-1 and myosin in myocardium after 4 months following implantation. (A, B) Projections of SDF-1 in the rat heart specimens from control and LAD-treated groups, respectively. (C, D) Relative intensities of immunofluorescence of SDF-1 shown in (A) and (B), respectively. Stem cells were labelled with EGFP (in green) as indicated in Materials and Methods. Fluorescent signals from SDF-1 and nuclei were collected in the red and blue channels, respectively. Localization of SDF-1 in EGFP-labelled cells is indicated with white arrows. (E, F) Projections of myosin (in red) in the rat heart specimens from control and LAD-treated groups, respectively. (G, H) Relative intensities of immunofluorescence of myosin shown in (E) and (F), respectively.

Mentions: In the second set of experiments we analysed the effect of the treatment of the EGFP-labelled cells with resveratrol on their engraftment and therapeutic efficacy after 4 months following the LAD occlusion and implantation. High number of the EGFP-expressing cells were consistently present in the myocardium when the cells were pre-treated with resveratrol as compared to control (Figs 2, 4, 5, in green). Moreover, we observed the resveratrol-induced prolonged alterations on the redox status in these cells that was determined by assessment of responses of nuclear factors Nrf2, Ref-1 and NFκB. The data presented in Figure 2A–C show that the nuclear localization of Nrf2 was significantly increased in the group of LAD occlusion followed by implant with resveratrol-treated cardiac stem cells (compared to the control group implanted with resveratrol-non-treated cells). The indexes of spatial correlation ‘r’ of Nrf2 immunoreactivity with nuclei for the projections presented in Figure 2A and B were 0.014 and 0.16, respectively. The EGFP-positive cells in the LAD occlusion specimens were characterized by a pronounced expression of Ki67 protein (Fig. 2E and F) compared to the control (Fig. 2D).


Regeneration of infarcted myocardium with resveratrol-modified cardiac stem cells.

Gorbunov N, Petrovski G, Gurusamy N, Ray D, Kim do H, Das DK - J. Cell. Mol. Med. (2012)

Immunofluorescence assessment of the combined effect of cardiac stem cells on the expression of SDF-1 and myosin in myocardium after 4 months following implantation. (A, B) Projections of SDF-1 in the rat heart specimens from control and LAD-treated groups, respectively. (C, D) Relative intensities of immunofluorescence of SDF-1 shown in (A) and (B), respectively. Stem cells were labelled with EGFP (in green) as indicated in Materials and Methods. Fluorescent signals from SDF-1 and nuclei were collected in the red and blue channels, respectively. Localization of SDF-1 in EGFP-labelled cells is indicated with white arrows. (E, F) Projections of myosin (in red) in the rat heart specimens from control and LAD-treated groups, respectively. (G, H) Relative intensities of immunofluorescence of myosin shown in (E) and (F), respectively.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3823103&req=5

fig05: Immunofluorescence assessment of the combined effect of cardiac stem cells on the expression of SDF-1 and myosin in myocardium after 4 months following implantation. (A, B) Projections of SDF-1 in the rat heart specimens from control and LAD-treated groups, respectively. (C, D) Relative intensities of immunofluorescence of SDF-1 shown in (A) and (B), respectively. Stem cells were labelled with EGFP (in green) as indicated in Materials and Methods. Fluorescent signals from SDF-1 and nuclei were collected in the red and blue channels, respectively. Localization of SDF-1 in EGFP-labelled cells is indicated with white arrows. (E, F) Projections of myosin (in red) in the rat heart specimens from control and LAD-treated groups, respectively. (G, H) Relative intensities of immunofluorescence of myosin shown in (E) and (F), respectively.
Mentions: In the second set of experiments we analysed the effect of the treatment of the EGFP-labelled cells with resveratrol on their engraftment and therapeutic efficacy after 4 months following the LAD occlusion and implantation. High number of the EGFP-expressing cells were consistently present in the myocardium when the cells were pre-treated with resveratrol as compared to control (Figs 2, 4, 5, in green). Moreover, we observed the resveratrol-induced prolonged alterations on the redox status in these cells that was determined by assessment of responses of nuclear factors Nrf2, Ref-1 and NFκB. The data presented in Figure 2A–C show that the nuclear localization of Nrf2 was significantly increased in the group of LAD occlusion followed by implant with resveratrol-treated cardiac stem cells (compared to the control group implanted with resveratrol-non-treated cells). The indexes of spatial correlation ‘r’ of Nrf2 immunoreactivity with nuclei for the projections presented in Figure 2A and B were 0.014 and 0.16, respectively. The EGFP-positive cells in the LAD occlusion specimens were characterized by a pronounced expression of Ki67 protein (Fig. 2E and F) compared to the control (Fig. 2D).

Bottom Line: Indeed, the vast majority of host-transfused cells do not survive beyond 24-72 hrs.Rats were anaesthetized, hearts opened and the LAD occluded to induce heart attack.One week later, the cardiac reduced environment was confirmed in resveratrol treated rat hearts by the enhanced expression of nuclear factor-E2-related factor-2 (Nrf2) and redox effector factor-1 (Ref-1).

View Article: PubMed Central - PubMed

Affiliation: The Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MD, USA.

Show MeSH
Related in: MedlinePlus