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Regeneration of infarcted myocardium with resveratrol-modified cardiac stem cells.

Gorbunov N, Petrovski G, Gurusamy N, Ray D, Kim do H, Das DK - J. Cell. Mol. Med. (2012)

Bottom Line: Indeed, the vast majority of host-transfused cells do not survive beyond 24-72 hrs.Rats were anaesthetized, hearts opened and the LAD occluded to induce heart attack.One week later, the cardiac reduced environment was confirmed in resveratrol treated rat hearts by the enhanced expression of nuclear factor-E2-related factor-2 (Nrf2) and redox effector factor-1 (Ref-1).

View Article: PubMed Central - PubMed

Affiliation: The Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MD, USA.

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Immunofluorescence assessment of Ref-1 response in cardiac tissue after 4 months following implantation of cardiac stem cells. (A–D) Control and (E–H) the LAD occlusion implanted with resveratrol-treated cell groups. Projections of Ref-1 and nuclear localization of Ref-1 in cardiac cells are shown in (C), (G) and (D), (H), respectively. Stem cells were labelled with EGFP as indicated in Materials and Methods. Localization of Ref-1 (in red) in the nuclei (in blue) is indicated with white arrows.
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fig03: Immunofluorescence assessment of Ref-1 response in cardiac tissue after 4 months following implantation of cardiac stem cells. (A–D) Control and (E–H) the LAD occlusion implanted with resveratrol-treated cell groups. Projections of Ref-1 and nuclear localization of Ref-1 in cardiac cells are shown in (C), (G) and (D), (H), respectively. Stem cells were labelled with EGFP as indicated in Materials and Methods. Localization of Ref-1 (in red) in the nuclei (in blue) is indicated with white arrows.

Mentions: Nuclear localization of Ref-1 in EGFP-positive implanted cells is shown in Figure 3. We have found that the resveratrol-treatment procedure results in a prolonged increase in the Ref-1/nuclear interaction in hearts subjected to infarction. Thus, the indexes of spatial correlation ‘r’ of Ref-1 immunoreactivity with nuclei for the projections presented in Figure 3D (control) and H (LAD occlusion) were 0.07 and 0.52, respectively. Similar effect was demonstrated for the p65 subunit of κB (Fig. 4A–C) that was accompanied by substantial elevation of Ki67 expression in EGFP-positive cells (Fig. 4D–F).


Regeneration of infarcted myocardium with resveratrol-modified cardiac stem cells.

Gorbunov N, Petrovski G, Gurusamy N, Ray D, Kim do H, Das DK - J. Cell. Mol. Med. (2012)

Immunofluorescence assessment of Ref-1 response in cardiac tissue after 4 months following implantation of cardiac stem cells. (A–D) Control and (E–H) the LAD occlusion implanted with resveratrol-treated cell groups. Projections of Ref-1 and nuclear localization of Ref-1 in cardiac cells are shown in (C), (G) and (D), (H), respectively. Stem cells were labelled with EGFP as indicated in Materials and Methods. Localization of Ref-1 (in red) in the nuclei (in blue) is indicated with white arrows.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3823103&req=5

fig03: Immunofluorescence assessment of Ref-1 response in cardiac tissue after 4 months following implantation of cardiac stem cells. (A–D) Control and (E–H) the LAD occlusion implanted with resveratrol-treated cell groups. Projections of Ref-1 and nuclear localization of Ref-1 in cardiac cells are shown in (C), (G) and (D), (H), respectively. Stem cells were labelled with EGFP as indicated in Materials and Methods. Localization of Ref-1 (in red) in the nuclei (in blue) is indicated with white arrows.
Mentions: Nuclear localization of Ref-1 in EGFP-positive implanted cells is shown in Figure 3. We have found that the resveratrol-treatment procedure results in a prolonged increase in the Ref-1/nuclear interaction in hearts subjected to infarction. Thus, the indexes of spatial correlation ‘r’ of Ref-1 immunoreactivity with nuclei for the projections presented in Figure 3D (control) and H (LAD occlusion) were 0.07 and 0.52, respectively. Similar effect was demonstrated for the p65 subunit of κB (Fig. 4A–C) that was accompanied by substantial elevation of Ki67 expression in EGFP-positive cells (Fig. 4D–F).

Bottom Line: Indeed, the vast majority of host-transfused cells do not survive beyond 24-72 hrs.Rats were anaesthetized, hearts opened and the LAD occluded to induce heart attack.One week later, the cardiac reduced environment was confirmed in resveratrol treated rat hearts by the enhanced expression of nuclear factor-E2-related factor-2 (Nrf2) and redox effector factor-1 (Ref-1).

View Article: PubMed Central - PubMed

Affiliation: The Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MD, USA.

Show MeSH
Related in: MedlinePlus