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Regeneration of infarcted myocardium with resveratrol-modified cardiac stem cells.

Gorbunov N, Petrovski G, Gurusamy N, Ray D, Kim do H, Das DK - J. Cell. Mol. Med. (2012)

Bottom Line: Indeed, the vast majority of host-transfused cells do not survive beyond 24-72 hrs.Rats were anaesthetized, hearts opened and the LAD occluded to induce heart attack.One week later, the cardiac reduced environment was confirmed in resveratrol treated rat hearts by the enhanced expression of nuclear factor-E2-related factor-2 (Nrf2) and redox effector factor-1 (Ref-1).

View Article: PubMed Central - PubMed

Affiliation: The Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MD, USA.

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Immunofluorescence assessment of redox signalling and proliferative activity of EGFP-positive cardiac stem cells at day 7 after implantation. (A–C) Projections of nuclear factors Nrf2 (in red), Ref-1 (in red), and p65-κB (in red) respectively in the rat heart specimens from animals subjected to LAD occlusion and implanted with resvera-trol-treated cell. EGFP cells appear in green. Localizations of Nrf2, Ref-1, p65-κB (in red) in the nuclei (in blue) are indicated with white arrows. (D) Projection of Ki67 in the rat heart specimens from animals subjected to LAD occlusion and implanted with resveratrol-treated cell. Localization of Ki67 (in red) is indicated with white arrows.
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fig01: Immunofluorescence assessment of redox signalling and proliferative activity of EGFP-positive cardiac stem cells at day 7 after implantation. (A–C) Projections of nuclear factors Nrf2 (in red), Ref-1 (in red), and p65-κB (in red) respectively in the rat heart specimens from animals subjected to LAD occlusion and implanted with resvera-trol-treated cell. EGFP cells appear in green. Localizations of Nrf2, Ref-1, p65-κB (in red) in the nuclei (in blue) are indicated with white arrows. (D) Projection of Ki67 in the rat heart specimens from animals subjected to LAD occlusion and implanted with resveratrol-treated cell. Localization of Ki67 (in red) is indicated with white arrows.

Mentions: The first set of experiments was conducted to verify the presence of effective homing and survival of the resveratrol-treated EGFP-labelled cells implanted to the hearts subjected to the LAD occlusion. With this goal we employed fluorescence confocal imaging for analysis of microsections of cardiac tissues harvested at day 3 (not shown) and day 7 following surgery and implantation. High number of the EGFP-expressing cells were observed in the myocardium at day 7 after LAD occlusion (Fig. 1A–D in green).


Regeneration of infarcted myocardium with resveratrol-modified cardiac stem cells.

Gorbunov N, Petrovski G, Gurusamy N, Ray D, Kim do H, Das DK - J. Cell. Mol. Med. (2012)

Immunofluorescence assessment of redox signalling and proliferative activity of EGFP-positive cardiac stem cells at day 7 after implantation. (A–C) Projections of nuclear factors Nrf2 (in red), Ref-1 (in red), and p65-κB (in red) respectively in the rat heart specimens from animals subjected to LAD occlusion and implanted with resvera-trol-treated cell. EGFP cells appear in green. Localizations of Nrf2, Ref-1, p65-κB (in red) in the nuclei (in blue) are indicated with white arrows. (D) Projection of Ki67 in the rat heart specimens from animals subjected to LAD occlusion and implanted with resveratrol-treated cell. Localization of Ki67 (in red) is indicated with white arrows.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3823103&req=5

fig01: Immunofluorescence assessment of redox signalling and proliferative activity of EGFP-positive cardiac stem cells at day 7 after implantation. (A–C) Projections of nuclear factors Nrf2 (in red), Ref-1 (in red), and p65-κB (in red) respectively in the rat heart specimens from animals subjected to LAD occlusion and implanted with resvera-trol-treated cell. EGFP cells appear in green. Localizations of Nrf2, Ref-1, p65-κB (in red) in the nuclei (in blue) are indicated with white arrows. (D) Projection of Ki67 in the rat heart specimens from animals subjected to LAD occlusion and implanted with resveratrol-treated cell. Localization of Ki67 (in red) is indicated with white arrows.
Mentions: The first set of experiments was conducted to verify the presence of effective homing and survival of the resveratrol-treated EGFP-labelled cells implanted to the hearts subjected to the LAD occlusion. With this goal we employed fluorescence confocal imaging for analysis of microsections of cardiac tissues harvested at day 3 (not shown) and day 7 following surgery and implantation. High number of the EGFP-expressing cells were observed in the myocardium at day 7 after LAD occlusion (Fig. 1A–D in green).

Bottom Line: Indeed, the vast majority of host-transfused cells do not survive beyond 24-72 hrs.Rats were anaesthetized, hearts opened and the LAD occluded to induce heart attack.One week later, the cardiac reduced environment was confirmed in resveratrol treated rat hearts by the enhanced expression of nuclear factor-E2-related factor-2 (Nrf2) and redox effector factor-1 (Ref-1).

View Article: PubMed Central - PubMed

Affiliation: The Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MD, USA.

Show MeSH
Related in: MedlinePlus