Intestinal metabolite compound K of panaxoside inhibits the growth of gastric carcinoma by augmenting apoptosis via Bid-mediated mitochondrial pathway.
Bottom Line: We found that CK significantly inhibits the viabilities of BGC823 and SGC7901 cells in dose- and time-dependent manners.CK-induced BGC823 and SGC7901 cells apoptosis and cell cycle arrest in G2 phase by up-regulation of p21 and down-regulation of cdc2 and cyclin B1.Further studies show that CK induces apoptosis in BGC823 and SGC7901 cells mainly through mitochondria-mediated internal pathway, and that CK induces the translocation of nuclear Bid to mitochondria.
Affiliation: Cancer Research Center, Xiamen University Medical College, Xiamen, China.Show MeSH
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Mentions: Hoechst-33342 and annexin-V/PI staining assays were combined to investigate whether CK can induce human gastric carcinoma cells apoptosis. First, we observed the apoptotic morphology of BGC823 and SGC7901 cells treated with 5.0 μmol/l CK for 24 hrs, and found that the CK-treated cells had manifest brighter granular blue fluorescence and more apoptotic bodies compared with control group (Fig. 2A). Furthermore, we assessed the apoptotic rate of the cells treated with different concentrations of CK (0, 2.5, 5.0, 7.5 and 10.0 μmol/l) for 24 hrs and 5.0 μmol/l CK for different time periods (0, 12, 24, 36 and 48 hrs) using annexin V/PI staining assay. As shown in Figure 2B and C, CK-induced apoptosis in BGC823 and SGC7901 cells in a dose- and time-dependent manner and BGC823 cells were more sensitive to CK treatment than SGC7901 cells.
Affiliation: Cancer Research Center, Xiamen University Medical College, Xiamen, China.