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Multifaceted role of nitric oxide in an in vitro mouse neuronal injury model: transcriptomic profiling defines the temporal recruitment of death signalling cascades.

Peng ZF, Chen MJ, Manikandan J, Melendez AJ, Shui G, Russo-Marie F, Whiteman M, Beart PM, Moore PK, Cheung NS - J. Cell. Mol. Med. (2012)

Bottom Line: Biological pathway analysis focused upon 3672 gene probes which demonstrated at least a ±1.5-fold expression in a minimum of one out of three time-points and passed statistical analysis (one-way anova, P < 0.05).Numerous enriched processes potentially determining nitric oxide mediated neuronal injury were identified from the transcriptomic profile: cell death, developmental growth and survival, cell cycle, calcium ion homeostasis, endoplasmic reticulum stress, oxidative stress, mitochondrial homeostasis, ubiquitin-mediated proteolysis, and GSH and nitric oxide metabolism.These data form a foundation for the development of screening platforms and define targets for intervention in nitric oxide neuropathologies where nitric oxide mediated injury is causative.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Biogeology and Environmental Geology of the Ministry of Education, China University of Geosciences, Wuhan, China.

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Flowchart of microarray analyses and profiling of neuronal death-related differentially expressed genes in nitric oxide mediated neuronal injury.
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fig04: Flowchart of microarray analyses and profiling of neuronal death-related differentially expressed genes in nitric oxide mediated neuronal injury.

Mentions: The global gene profile list representative of NOC-18-mediated neuronal injury (3672 microarray chip-annotated probes sets) was then subjected to functional-gene ontology classification by DAVID 6.7 analysis [28, 29]. DAVID interpretation recognized 3484 biologically and functionally reported genes from various biological databases for nitric oxide treatment (as shown in Fig. 4).


Multifaceted role of nitric oxide in an in vitro mouse neuronal injury model: transcriptomic profiling defines the temporal recruitment of death signalling cascades.

Peng ZF, Chen MJ, Manikandan J, Melendez AJ, Shui G, Russo-Marie F, Whiteman M, Beart PM, Moore PK, Cheung NS - J. Cell. Mol. Med. (2012)

Flowchart of microarray analyses and profiling of neuronal death-related differentially expressed genes in nitric oxide mediated neuronal injury.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3823092&req=5

fig04: Flowchart of microarray analyses and profiling of neuronal death-related differentially expressed genes in nitric oxide mediated neuronal injury.
Mentions: The global gene profile list representative of NOC-18-mediated neuronal injury (3672 microarray chip-annotated probes sets) was then subjected to functional-gene ontology classification by DAVID 6.7 analysis [28, 29]. DAVID interpretation recognized 3484 biologically and functionally reported genes from various biological databases for nitric oxide treatment (as shown in Fig. 4).

Bottom Line: Biological pathway analysis focused upon 3672 gene probes which demonstrated at least a ±1.5-fold expression in a minimum of one out of three time-points and passed statistical analysis (one-way anova, P < 0.05).Numerous enriched processes potentially determining nitric oxide mediated neuronal injury were identified from the transcriptomic profile: cell death, developmental growth and survival, cell cycle, calcium ion homeostasis, endoplasmic reticulum stress, oxidative stress, mitochondrial homeostasis, ubiquitin-mediated proteolysis, and GSH and nitric oxide metabolism.These data form a foundation for the development of screening platforms and define targets for intervention in nitric oxide neuropathologies where nitric oxide mediated injury is causative.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Biogeology and Environmental Geology of the Ministry of Education, China University of Geosciences, Wuhan, China.

Show MeSH
Related in: MedlinePlus