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Lucifer yellow - an angel rather than the devil.

Hanani M - J. Cell. Mol. Med. (2012)

Bottom Line: This dye has been used mostly for labelling cells by intracellular injection from microelectrodes.This review describes the numerous applications of LY, with emphasis on the enteric nervous system and interstitial cells of Cajal.Of particular importance is the dye coupling method, which enables the detection of cell coupling by gap junctions.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Experimental Surgery, Hadassah-Hebrew University Medical Center, Mount Scopus, Jerusalem, Israel. hananim@cc.huji.ac.il

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Confocal microscopy of satellite glial cells in the guinea-pig dorsal root ganglion. (A) A single confocal slice. LY was injected into the cell marked with an asterisk, and the dye spread into other glial cells that make an envelope around a sensory neuron (which is not labelled). Some of the glial cells are indicated with an arrow. (B) Three-dimensional reconstruction of the cells shown in (A). (C) The same cells shown in (B) are displayed at 90° rotation. Calibration bar = 50 μm.
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fig03: Confocal microscopy of satellite glial cells in the guinea-pig dorsal root ganglion. (A) A single confocal slice. LY was injected into the cell marked with an asterisk, and the dye spread into other glial cells that make an envelope around a sensory neuron (which is not labelled). Some of the glial cells are indicated with an arrow. (B) Three-dimensional reconstruction of the cells shown in (A). (C) The same cells shown in (B) are displayed at 90° rotation. Calibration bar = 50 μm.

Mentions: Early work has shown that injecting single cells with LY provides a picture of the cells with extraordinary detail. The image by Stewart [13] of an LY-injected leech neuron on the cover of Nature is very memorable. Apparently LY can diffuse into very fine processes, providing highly detailed picture of cell morphology, which resembles Golgi staining. However, in contrast with the Golgi method, which is highly variable and unpredictable, LY injection is quite predictable. This is in addition to the ability to learn about the physiology of the injected cell. With the advent of con-focal microcopy and three-dimensional reconstruction, LY labelling yields extremely fine detail of cell morphology, for example Refs. [39–41]. Labelling with LY in combination with immuno-histochemistry has a great added value. Examples of intracellular labelling of neurons and an ICC are shown in Figure 2. Confocal images of LY-labelled satellite glial cells in dorsal root ganglion are shown in Figure 3.


Lucifer yellow - an angel rather than the devil.

Hanani M - J. Cell. Mol. Med. (2012)

Confocal microscopy of satellite glial cells in the guinea-pig dorsal root ganglion. (A) A single confocal slice. LY was injected into the cell marked with an asterisk, and the dye spread into other glial cells that make an envelope around a sensory neuron (which is not labelled). Some of the glial cells are indicated with an arrow. (B) Three-dimensional reconstruction of the cells shown in (A). (C) The same cells shown in (B) are displayed at 90° rotation. Calibration bar = 50 μm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3823090&req=5

fig03: Confocal microscopy of satellite glial cells in the guinea-pig dorsal root ganglion. (A) A single confocal slice. LY was injected into the cell marked with an asterisk, and the dye spread into other glial cells that make an envelope around a sensory neuron (which is not labelled). Some of the glial cells are indicated with an arrow. (B) Three-dimensional reconstruction of the cells shown in (A). (C) The same cells shown in (B) are displayed at 90° rotation. Calibration bar = 50 μm.
Mentions: Early work has shown that injecting single cells with LY provides a picture of the cells with extraordinary detail. The image by Stewart [13] of an LY-injected leech neuron on the cover of Nature is very memorable. Apparently LY can diffuse into very fine processes, providing highly detailed picture of cell morphology, which resembles Golgi staining. However, in contrast with the Golgi method, which is highly variable and unpredictable, LY injection is quite predictable. This is in addition to the ability to learn about the physiology of the injected cell. With the advent of con-focal microcopy and three-dimensional reconstruction, LY labelling yields extremely fine detail of cell morphology, for example Refs. [39–41]. Labelling with LY in combination with immuno-histochemistry has a great added value. Examples of intracellular labelling of neurons and an ICC are shown in Figure 2. Confocal images of LY-labelled satellite glial cells in dorsal root ganglion are shown in Figure 3.

Bottom Line: This dye has been used mostly for labelling cells by intracellular injection from microelectrodes.This review describes the numerous applications of LY, with emphasis on the enteric nervous system and interstitial cells of Cajal.Of particular importance is the dye coupling method, which enables the detection of cell coupling by gap junctions.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Experimental Surgery, Hadassah-Hebrew University Medical Center, Mount Scopus, Jerusalem, Israel. hananim@cc.huji.ac.il

Show MeSH
Related in: MedlinePlus