Lucifer yellow - an angel rather than the devil.
Bottom Line: The fluorescent dye Lucifer yellow (LY) was introduced in 1978, and has been extremely useful in studying cell structure and communications.This dye has been used mostly for labelling cells by intracellular injection from microelectrodes.This review describes the numerous applications of LY, with emphasis on the enteric nervous system and interstitial cells of Cajal.
Affiliation: Laboratory of Experimental Surgery, Hadassah-Hebrew University Medical Center, Mount Scopus, Jerusalem, Israel. email@example.comShow MeSH
Related in: MedlinePlus
Mentions: Early work has shown that injecting single cells with LY provides a picture of the cells with extraordinary detail. The image by Stewart  of an LY-injected leech neuron on the cover of Nature is very memorable. Apparently LY can diffuse into very fine processes, providing highly detailed picture of cell morphology, which resembles Golgi staining. However, in contrast with the Golgi method, which is highly variable and unpredictable, LY injection is quite predictable. This is in addition to the ability to learn about the physiology of the injected cell. With the advent of con-focal microcopy and three-dimensional reconstruction, LY labelling yields extremely fine detail of cell morphology, for example Refs. [39–41]. Labelling with LY in combination with immuno-histochemistry has a great added value. Examples of intracellular labelling of neurons and an ICC are shown in Figure 2. Confocal images of LY-labelled satellite glial cells in dorsal root ganglion are shown in Figure 3.
Affiliation: Laboratory of Experimental Surgery, Hadassah-Hebrew University Medical Center, Mount Scopus, Jerusalem, Israel. firstname.lastname@example.org