A new oncolytic adenoviral vector carrying dual tumour suppressor genes shows potent anti-tumour effect.
Bottom Line: Our results demonstrated excellent anti-tumour effect of ZD55SP/E1A-IL-24-shMPP1 in vitro on multiple cancer cell lines such as lung cancer, liver cancer and ovarian caner.More importantly, ZD55SP/E1A-IL-24-shMPP1 also showed excellent anti-tumour effects in vivo on SW620 xenograft nude mice.In conclusion, our strategy of constructing an IL-24 and shMPP1 dual gene expressing oncolytic adenoviral vector, which is regulated by the survivin promoter and E1B55KD deletion, could be a promising method of cancer gene therapy.
Affiliation: Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.Show MeSH
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Mentions: The OV ZD55SP/E1A is constructed based on the ZD55 vector . In this study, the E1A promoter in the original wild-type serotype 5 Ad was replaced by the short 269 bp survivin promoter, and the E1B55KD was also deleted. Expression cassettes of both IL-24 and shMPP1 were then inserted into this new vector (Fig. 3A).
Affiliation: Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.