A new oncolytic adenoviral vector carrying dual tumour suppressor genes shows potent anti-tumour effect.
Bottom Line: Compared with the original ZD55 vector, this new adenoviral vector (ZD55SP/E1A) showed much better ability of replication and reporter gene expression.Our results demonstrated excellent anti-tumour effect of ZD55SP/E1A-IL-24-shMPP1 in vitro on multiple cancer cell lines such as lung cancer, liver cancer and ovarian caner.More importantly, ZD55SP/E1A-IL-24-shMPP1 also showed excellent anti-tumour effects in vivo on SW620 xenograft nude mice.
Affiliation: Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.Show MeSH
Related in: MedlinePlus
Mentions: The OV ZD55SP/E1A is constructed based on the ZD55 vector . In this study, the E1A promoter in the original wild-type serotype 5 Ad was replaced by the short 269 bp survivin promoter, and the E1B55KD was also deleted. Expression cassettes of both IL-24 and shMPP1 were then inserted into this new vector (Fig. 3A).
Affiliation: Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.