A new oncolytic adenoviral vector carrying dual tumour suppressor genes shows potent anti-tumour effect.
Bottom Line: Our results demonstrated excellent anti-tumour effect of ZD55SP/E1A-IL-24-shMPP1 in vitro on multiple cancer cell lines such as lung cancer, liver cancer and ovarian caner.More importantly, ZD55SP/E1A-IL-24-shMPP1 also showed excellent anti-tumour effects in vivo on SW620 xenograft nude mice.In conclusion, our strategy of constructing an IL-24 and shMPP1 dual gene expressing oncolytic adenoviral vector, which is regulated by the survivin promoter and E1B55KD deletion, could be a promising method of cancer gene therapy.
Affiliation: Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.Show MeSH
Related in: MedlinePlus
Mentions: It has been reported that MPHOSPH1 is up-regulated in bladder cancer cells but not in normal human tissues . To evaluate MPHOSPH1 levels in different cancer cells, we employed quantitative real-time PCR to analyse the levels of MPHOSPH1 mRNA. We found that MPHOSPH1 was up-regulated not only in the bladder cancer cells (SCaBER and T24 cells), but also in many other types of cancer (HeLa, A549, BEL-7404 and SW620 cells); whereas it remained at low levels in normal cells (MRC-5 and L-02 cells; Fig. 2), which suggested MPHOSPH1 may be an ideal target in cancer gene therapy.
Affiliation: Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.