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A new oncolytic adenoviral vector carrying dual tumour suppressor genes shows potent anti-tumour effect.

Liu XR, Cai Y, Cao X, Wei RC, Li HL, Zhou XM, Zhang KJ, Wu S, Qian QJ, Cheng B, Huang K, Liu XY - J. Cell. Mol. Med. (2012)

Bottom Line: Compared with the original ZD55 vector, this new adenoviral vector (ZD55SP/E1A) showed much better ability of replication and reporter gene expression.Our results demonstrated excellent anti-tumour effect of ZD55SP/E1A-IL-24-shMPP1 in vitro on multiple cancer cell lines such as lung cancer, liver cancer and ovarian caner.More importantly, ZD55SP/E1A-IL-24-shMPP1 also showed excellent anti-tumour effects in vivo on SW620 xenograft nude mice.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.

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Related in: MedlinePlus

Quantification of the MPHOSPH1 transcript level in various cell lines. The relative levels of MPHOSPH1 mRNA in five tumour and two normal cell lines were examined by RT-PCR. GAPDH was used as an internal control. Data are presented as mean ± S.D. (bars) (n ∇ 3).
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fig02: Quantification of the MPHOSPH1 transcript level in various cell lines. The relative levels of MPHOSPH1 mRNA in five tumour and two normal cell lines were examined by RT-PCR. GAPDH was used as an internal control. Data are presented as mean ± S.D. (bars) (n ∇ 3).

Mentions: It has been reported that MPHOSPH1 is up-regulated in bladder cancer cells but not in normal human tissues [4]. To evaluate MPHOSPH1 levels in different cancer cells, we employed quantitative real-time PCR to analyse the levels of MPHOSPH1 mRNA. We found that MPHOSPH1 was up-regulated not only in the bladder cancer cells (SCaBER and T24 cells), but also in many other types of cancer (HeLa, A549, BEL-7404 and SW620 cells); whereas it remained at low levels in normal cells (MRC-5 and L-02 cells; Fig. 2), which suggested MPHOSPH1 may be an ideal target in cancer gene therapy.


A new oncolytic adenoviral vector carrying dual tumour suppressor genes shows potent anti-tumour effect.

Liu XR, Cai Y, Cao X, Wei RC, Li HL, Zhou XM, Zhang KJ, Wu S, Qian QJ, Cheng B, Huang K, Liu XY - J. Cell. Mol. Med. (2012)

Quantification of the MPHOSPH1 transcript level in various cell lines. The relative levels of MPHOSPH1 mRNA in five tumour and two normal cell lines were examined by RT-PCR. GAPDH was used as an internal control. Data are presented as mean ± S.D. (bars) (n ∇ 3).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3823082&req=5

fig02: Quantification of the MPHOSPH1 transcript level in various cell lines. The relative levels of MPHOSPH1 mRNA in five tumour and two normal cell lines were examined by RT-PCR. GAPDH was used as an internal control. Data are presented as mean ± S.D. (bars) (n ∇ 3).
Mentions: It has been reported that MPHOSPH1 is up-regulated in bladder cancer cells but not in normal human tissues [4]. To evaluate MPHOSPH1 levels in different cancer cells, we employed quantitative real-time PCR to analyse the levels of MPHOSPH1 mRNA. We found that MPHOSPH1 was up-regulated not only in the bladder cancer cells (SCaBER and T24 cells), but also in many other types of cancer (HeLa, A549, BEL-7404 and SW620 cells); whereas it remained at low levels in normal cells (MRC-5 and L-02 cells; Fig. 2), which suggested MPHOSPH1 may be an ideal target in cancer gene therapy.

Bottom Line: Compared with the original ZD55 vector, this new adenoviral vector (ZD55SP/E1A) showed much better ability of replication and reporter gene expression.Our results demonstrated excellent anti-tumour effect of ZD55SP/E1A-IL-24-shMPP1 in vitro on multiple cancer cell lines such as lung cancer, liver cancer and ovarian caner.More importantly, ZD55SP/E1A-IL-24-shMPP1 also showed excellent anti-tumour effects in vivo on SW620 xenograft nude mice.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.

Show MeSH
Related in: MedlinePlus