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Comparison of hemagglutination inhibition assay and enzyme immunoassay for determination of mumps and rubella immune status in health care personnel.

Kumakura S, Shibata H, Isobe T, Hirose M, Ohe M, Nishimura N, Onoda K, Nagai A, Yamaguchi S - J. Clin. Lab. Anal. (2013)

Bottom Line: A total of 910 sera from health care personnel were tested.As compared with EIA, HI identified sixfold larger seronegative subjects in mumps.Moreover, in mumps, 88.8% of seronegative subjects detected by HI were seropositive by EIA, while 3.7% of seropositive subjects detected by HI were seronegative by EIA.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Education and Research, Faculty of Medicine, Shimane University, Izumo, Japan; Infection Control Committee, Faculty of Medicine, Shimane University, Izumo, Japan.

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Scattergrams of rubella titers detected by HI versus EIA.The antibody titers against rubella of health care personnel (n = 910) detected by HI were compared with those by EIA.
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fig02: Scattergrams of rubella titers detected by HI versus EIA.The antibody titers against rubella of health care personnel (n = 910) detected by HI were compared with those by EIA.

Mentions: Scattergrams that compare mumps and rubella antibody titers obtained by HI to those by EIA for individuals of 910 health care personnel are shown in Figures 1 and 2, respectively. In mumps, the titers of HI were poorly correlated with those of EIA, and correlation coefficient values (r) were calculated at 0.190 (P < 0.001; Fig. 1) whereas, rubella titers correlated relatively well with HI and EIA (r = 0.930, P < 0.001; Fig. 2).


Comparison of hemagglutination inhibition assay and enzyme immunoassay for determination of mumps and rubella immune status in health care personnel.

Kumakura S, Shibata H, Isobe T, Hirose M, Ohe M, Nishimura N, Onoda K, Nagai A, Yamaguchi S - J. Clin. Lab. Anal. (2013)

Scattergrams of rubella titers detected by HI versus EIA.The antibody titers against rubella of health care personnel (n = 910) detected by HI were compared with those by EIA.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3823067&req=5

fig02: Scattergrams of rubella titers detected by HI versus EIA.The antibody titers against rubella of health care personnel (n = 910) detected by HI were compared with those by EIA.
Mentions: Scattergrams that compare mumps and rubella antibody titers obtained by HI to those by EIA for individuals of 910 health care personnel are shown in Figures 1 and 2, respectively. In mumps, the titers of HI were poorly correlated with those of EIA, and correlation coefficient values (r) were calculated at 0.190 (P < 0.001; Fig. 1) whereas, rubella titers correlated relatively well with HI and EIA (r = 0.930, P < 0.001; Fig. 2).

Bottom Line: A total of 910 sera from health care personnel were tested.As compared with EIA, HI identified sixfold larger seronegative subjects in mumps.Moreover, in mumps, 88.8% of seronegative subjects detected by HI were seropositive by EIA, while 3.7% of seropositive subjects detected by HI were seronegative by EIA.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Education and Research, Faculty of Medicine, Shimane University, Izumo, Japan; Infection Control Committee, Faculty of Medicine, Shimane University, Izumo, Japan.

Show MeSH
Related in: MedlinePlus