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Altered functional differentiation of mesoangioblasts in a genetic myopathy.

Altomare C, Barile L, Rocchetti M, Sala L, Crippa S, Sampaolesi M, Zaza A - J. Cell. Mol. Med. (2013)

Bottom Line: To this end, cMabs were compared with neonatal cardiomyocytes (CMs) and skeletal muscle C2C12 cells, representative of cardiac and skeletal muscle respectively.Contrary to previous reports, wild-type cMabs failed to show functional differentiation towards either cell type.We conclude that miRNA669a expression, ablated by βSG deletion, may prevent functional differentiation of cMabs towards the skeletal muscle phenotype.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnologies and Biosciences, University of Milano-Bicocca, Milan, Italy.

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Related in: MedlinePlus

Elementary Ca2+ release events. Examples of elementary Ca2+ release events recorded from quiescent βSG−/−, C2C12 myotubes and CM; Top: 3D representation of line-scan images. Middle: original-line-scan images in colour scale (right); Bottom: [Ca2+] time course of the events shown in the middle panel (embers and sparks, see text for definitions).
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fig07: Elementary Ca2+ release events. Examples of elementary Ca2+ release events recorded from quiescent βSG−/−, C2C12 myotubes and CM; Top: 3D representation of line-scan images. Middle: original-line-scan images in colour scale (right); Bottom: [Ca2+] time course of the events shown in the middle panel (embers and sparks, see text for definitions).

Mentions: Representative examples of Ca2+ sparks recordings in the three cell types are shown in Figure 7. The upper panel shows a 3D plot, in which normalized fluorescence intensity (amplitude) was digitized and represented on the vertical axis. Lower panels show raw-line-scan images (intensity represented by colour scale), aligned with the respective intensity profiles at the active release sites. Visual analysis of these examples reveals events with distinctly different durations; whereas short events prevailed in CMs and long ones in βSG−/− myotubes, C2C12 myotubes were characterized by the presence of both short and long events, appearing at distinct release sites.


Altered functional differentiation of mesoangioblasts in a genetic myopathy.

Altomare C, Barile L, Rocchetti M, Sala L, Crippa S, Sampaolesi M, Zaza A - J. Cell. Mol. Med. (2013)

Elementary Ca2+ release events. Examples of elementary Ca2+ release events recorded from quiescent βSG−/−, C2C12 myotubes and CM; Top: 3D representation of line-scan images. Middle: original-line-scan images in colour scale (right); Bottom: [Ca2+] time course of the events shown in the middle panel (embers and sparks, see text for definitions).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3823023&req=5

fig07: Elementary Ca2+ release events. Examples of elementary Ca2+ release events recorded from quiescent βSG−/−, C2C12 myotubes and CM; Top: 3D representation of line-scan images. Middle: original-line-scan images in colour scale (right); Bottom: [Ca2+] time course of the events shown in the middle panel (embers and sparks, see text for definitions).
Mentions: Representative examples of Ca2+ sparks recordings in the three cell types are shown in Figure 7. The upper panel shows a 3D plot, in which normalized fluorescence intensity (amplitude) was digitized and represented on the vertical axis. Lower panels show raw-line-scan images (intensity represented by colour scale), aligned with the respective intensity profiles at the active release sites. Visual analysis of these examples reveals events with distinctly different durations; whereas short events prevailed in CMs and long ones in βSG−/− myotubes, C2C12 myotubes were characterized by the presence of both short and long events, appearing at distinct release sites.

Bottom Line: To this end, cMabs were compared with neonatal cardiomyocytes (CMs) and skeletal muscle C2C12 cells, representative of cardiac and skeletal muscle respectively.Contrary to previous reports, wild-type cMabs failed to show functional differentiation towards either cell type.We conclude that miRNA669a expression, ablated by βSG deletion, may prevent functional differentiation of cMabs towards the skeletal muscle phenotype.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnologies and Biosciences, University of Milano-Bicocca, Milan, Italy.

Show MeSH
Related in: MedlinePlus