Altered functional differentiation of mesoangioblasts in a genetic myopathy.
Bottom Line: To this end, cMabs were compared with neonatal cardiomyocytes (CMs) and skeletal muscle C2C12 cells, representative of cardiac and skeletal muscle respectively.Contrary to previous reports, wild-type cMabs failed to show functional differentiation towards either cell type.We conclude that miRNA669a expression, ablated by βSG deletion, may prevent functional differentiation of cMabs towards the skeletal muscle phenotype.
Affiliation: Department of Biotechnologies and Biosciences, University of Milano-Bicocca, Milan, Italy.Show MeSH
Related in: MedlinePlus
Mentions: Representative examples of Ca2+ sparks recordings in the three cell types are shown in Figure 7. The upper panel shows a 3D plot, in which normalized fluorescence intensity (amplitude) was digitized and represented on the vertical axis. Lower panels show raw-line-scan images (intensity represented by colour scale), aligned with the respective intensity profiles at the active release sites. Visual analysis of these examples reveals events with distinctly different durations; whereas short events prevailed in CMs and long ones in βSG−/− myotubes, C2C12 myotubes were characterized by the presence of both short and long events, appearing at distinct release sites.
Affiliation: Department of Biotechnologies and Biosciences, University of Milano-Bicocca, Milan, Italy.