Altered functional differentiation of mesoangioblasts in a genetic myopathy.
Bottom Line: To this end, cMabs were compared with neonatal cardiomyocytes (CMs) and skeletal muscle C2C12 cells, representative of cardiac and skeletal muscle respectively.Contrary to previous reports, wild-type cMabs failed to show functional differentiation towards either cell type.We conclude that miRNA669a expression, ablated by βSG deletion, may prevent functional differentiation of cMabs towards the skeletal muscle phenotype.
Affiliation: Department of Biotechnologies and Biosciences, University of Milano-Bicocca, Milan, Italy.Show MeSH
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Mentions: As already evident by gross examination of sample recordings (Fig. 5a), inactivation kinetics was similar in βSG−/− and C2C12 myotubes and differed from that of CMs (also notice the difference in the timescale). Figure 5b shows that inactivation time constants (τinact) had a shallow dependency on membrane potential in all cell types, but were distinctly shorter in CMs (n = 9) than in βSG−/− or C2C12 myotubes (P < 0.05 at all potentials, n = 7 and n = 12 respectively).
Affiliation: Department of Biotechnologies and Biosciences, University of Milano-Bicocca, Milan, Italy.