Neurosensory mechanotransduction through acid-sensing ion channels.
Bottom Line: Thus, ASIC channels alone are not sufficient to reconstruct the path of transducing molecules of mechanically activated channels.The mechanotransducers associated with ASICs need further elucidation.Also we propose a molecular model and a new approach to disclose the molecular mechanism underlying the neurosensory mechanotransduction.
Affiliation: Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan. firstname.lastname@example.orgShow MeSH
Related in: MedlinePlus
Mentions: To examine neurosensory mechanotransduction in vitro, the choice of a cell-base technique is critical for testing the hypotheses grounded in mechanobiology . To probe neurosensory mechanotransduction, we recently developed a cell-based method to culture neurite-bearing DRG neurons on an ECM-coated elastomeric substrate . In such conditions, we can stretch a single neurite by substrate indentation without contacting the cell membrane. We can use whole-cell patch clamp recording to probe the neurite-stretch-induced mechanotransduction within a defined substrate, whose physical and molecular context can be modified to mimic physiologically relevant conditions (Fig. 2). This cell-based technique allows us to decode the ECM-tethered mechanosensitive ion channels. Accordingly, the neurite-stretch-induced action potential was present only in specific subsets of DRG neurons . Further studies on ASIC- mutations would be promising in determining ASIC isoforms as the ECM-tethered mechanosensitive ion channels in sensory nerves.
Affiliation: Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan. email@example.com