Limits...
Neurosensory mechanotransduction through acid-sensing ion channels.

Chen CC, Wong CW - J. Cell. Mol. Med. (2013)

Bottom Line: The mechanotransducers associated with ASICs need further elucidation.In this review, we discuss the expression of ASICs in sensory afferents of mechanoreceptors, findings of knockout studies, technical issues concerning studies of neurosensory mechanotransduction and possible missing links.Also we propose a molecular model and a new approach to disclose the molecular mechanism underlying the neurosensory mechanotransduction.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan. chih@ibms.sinica.edu.tw

Show MeSH

Related in: MedlinePlus

Probing sensory nerve mechanotransduction via localized elastomeric matrix control. Mechanical stretching imposed on a neurite via surface-modified elastomeric matrices. To probe the extracellular matrix (ECM)-tethered mechanotransduction on nerve terminals, neurite-bearing dorsal root ganglia neurons are cultured on an ECM-coated polydimethylsiloxane substrate that mimics a physiologically relevant elastic modulus (10–100 kPa). We can use whole-cell patch clamp recording to measure the electrical responses of neurosensory mechanotransduction on a single neurite by substrate indentation at a location adjacent to the neurite, which will deform the substrate and thus stretch the neurite without contacting it. This figure was modified from [82].
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3823015&req=5

fig02: Probing sensory nerve mechanotransduction via localized elastomeric matrix control. Mechanical stretching imposed on a neurite via surface-modified elastomeric matrices. To probe the extracellular matrix (ECM)-tethered mechanotransduction on nerve terminals, neurite-bearing dorsal root ganglia neurons are cultured on an ECM-coated polydimethylsiloxane substrate that mimics a physiologically relevant elastic modulus (10–100 kPa). We can use whole-cell patch clamp recording to measure the electrical responses of neurosensory mechanotransduction on a single neurite by substrate indentation at a location adjacent to the neurite, which will deform the substrate and thus stretch the neurite without contacting it. This figure was modified from [82].

Mentions: To examine neurosensory mechanotransduction in vitro, the choice of a cell-base technique is critical for testing the hypotheses grounded in mechanobiology [81]. To probe neurosensory mechanotransduction, we recently developed a cell-based method to culture neurite-bearing DRG neurons on an ECM-coated elastomeric substrate [82]. In such conditions, we can stretch a single neurite by substrate indentation without contacting the cell membrane. We can use whole-cell patch clamp recording to probe the neurite-stretch-induced mechanotransduction within a defined substrate, whose physical and molecular context can be modified to mimic physiologically relevant conditions (Fig. 2). This cell-based technique allows us to decode the ECM-tethered mechanosensitive ion channels. Accordingly, the neurite-stretch-induced action potential was present only in specific subsets of DRG neurons [78]. Further studies on ASIC- mutations would be promising in determining ASIC isoforms as the ECM-tethered mechanosensitive ion channels in sensory nerves.


Neurosensory mechanotransduction through acid-sensing ion channels.

Chen CC, Wong CW - J. Cell. Mol. Med. (2013)

Probing sensory nerve mechanotransduction via localized elastomeric matrix control. Mechanical stretching imposed on a neurite via surface-modified elastomeric matrices. To probe the extracellular matrix (ECM)-tethered mechanotransduction on nerve terminals, neurite-bearing dorsal root ganglia neurons are cultured on an ECM-coated polydimethylsiloxane substrate that mimics a physiologically relevant elastic modulus (10–100 kPa). We can use whole-cell patch clamp recording to measure the electrical responses of neurosensory mechanotransduction on a single neurite by substrate indentation at a location adjacent to the neurite, which will deform the substrate and thus stretch the neurite without contacting it. This figure was modified from [82].
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3823015&req=5

fig02: Probing sensory nerve mechanotransduction via localized elastomeric matrix control. Mechanical stretching imposed on a neurite via surface-modified elastomeric matrices. To probe the extracellular matrix (ECM)-tethered mechanotransduction on nerve terminals, neurite-bearing dorsal root ganglia neurons are cultured on an ECM-coated polydimethylsiloxane substrate that mimics a physiologically relevant elastic modulus (10–100 kPa). We can use whole-cell patch clamp recording to measure the electrical responses of neurosensory mechanotransduction on a single neurite by substrate indentation at a location adjacent to the neurite, which will deform the substrate and thus stretch the neurite without contacting it. This figure was modified from [82].
Mentions: To examine neurosensory mechanotransduction in vitro, the choice of a cell-base technique is critical for testing the hypotheses grounded in mechanobiology [81]. To probe neurosensory mechanotransduction, we recently developed a cell-based method to culture neurite-bearing DRG neurons on an ECM-coated elastomeric substrate [82]. In such conditions, we can stretch a single neurite by substrate indentation without contacting the cell membrane. We can use whole-cell patch clamp recording to probe the neurite-stretch-induced mechanotransduction within a defined substrate, whose physical and molecular context can be modified to mimic physiologically relevant conditions (Fig. 2). This cell-based technique allows us to decode the ECM-tethered mechanosensitive ion channels. Accordingly, the neurite-stretch-induced action potential was present only in specific subsets of DRG neurons [78]. Further studies on ASIC- mutations would be promising in determining ASIC isoforms as the ECM-tethered mechanosensitive ion channels in sensory nerves.

Bottom Line: The mechanotransducers associated with ASICs need further elucidation.In this review, we discuss the expression of ASICs in sensory afferents of mechanoreceptors, findings of knockout studies, technical issues concerning studies of neurosensory mechanotransduction and possible missing links.Also we propose a molecular model and a new approach to disclose the molecular mechanism underlying the neurosensory mechanotransduction.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan. chih@ibms.sinica.edu.tw

Show MeSH
Related in: MedlinePlus