Neurosensory mechanotransduction through acid-sensing ion channels.
Bottom Line: The mechanotransducers associated with ASICs need further elucidation.In this review, we discuss the expression of ASICs in sensory afferents of mechanoreceptors, findings of knockout studies, technical issues concerning studies of neurosensory mechanotransduction and possible missing links.Also we propose a molecular model and a new approach to disclose the molecular mechanism underlying the neurosensory mechanotransduction.
Affiliation: Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan. email@example.comShow MeSH
Related in: MedlinePlus
Mentions: To examine neurosensory mechanotransduction in vitro, the choice of a cell-base technique is critical for testing the hypotheses grounded in mechanobiology . To probe neurosensory mechanotransduction, we recently developed a cell-based method to culture neurite-bearing DRG neurons on an ECM-coated elastomeric substrate . In such conditions, we can stretch a single neurite by substrate indentation without contacting the cell membrane. We can use whole-cell patch clamp recording to probe the neurite-stretch-induced mechanotransduction within a defined substrate, whose physical and molecular context can be modified to mimic physiologically relevant conditions (Fig. 2). This cell-based technique allows us to decode the ECM-tethered mechanosensitive ion channels. Accordingly, the neurite-stretch-induced action potential was present only in specific subsets of DRG neurons . Further studies on ASIC- mutations would be promising in determining ASIC isoforms as the ECM-tethered mechanosensitive ion channels in sensory nerves.
Affiliation: Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan. firstname.lastname@example.org