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Neurosensory mechanotransduction through acid-sensing ion channels.

Chen CC, Wong CW - J. Cell. Mol. Med. (2013)

Bottom Line: Thus, ASIC channels alone are not sufficient to reconstruct the path of transducing molecules of mechanically activated channels.The mechanotransducers associated with ASICs need further elucidation.Also we propose a molecular model and a new approach to disclose the molecular mechanism underlying the neurosensory mechanotransduction.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan. chih@ibms.sinica.edu.tw

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Probing sensory nerve mechanotransduction via localized elastomeric matrix control. Mechanical stretching imposed on a neurite via surface-modified elastomeric matrices. To probe the extracellular matrix (ECM)-tethered mechanotransduction on nerve terminals, neurite-bearing dorsal root ganglia neurons are cultured on an ECM-coated polydimethylsiloxane substrate that mimics a physiologically relevant elastic modulus (10–100 kPa). We can use whole-cell patch clamp recording to measure the electrical responses of neurosensory mechanotransduction on a single neurite by substrate indentation at a location adjacent to the neurite, which will deform the substrate and thus stretch the neurite without contacting it. This figure was modified from [82].
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fig02: Probing sensory nerve mechanotransduction via localized elastomeric matrix control. Mechanical stretching imposed on a neurite via surface-modified elastomeric matrices. To probe the extracellular matrix (ECM)-tethered mechanotransduction on nerve terminals, neurite-bearing dorsal root ganglia neurons are cultured on an ECM-coated polydimethylsiloxane substrate that mimics a physiologically relevant elastic modulus (10–100 kPa). We can use whole-cell patch clamp recording to measure the electrical responses of neurosensory mechanotransduction on a single neurite by substrate indentation at a location adjacent to the neurite, which will deform the substrate and thus stretch the neurite without contacting it. This figure was modified from [82].

Mentions: To examine neurosensory mechanotransduction in vitro, the choice of a cell-base technique is critical for testing the hypotheses grounded in mechanobiology [81]. To probe neurosensory mechanotransduction, we recently developed a cell-based method to culture neurite-bearing DRG neurons on an ECM-coated elastomeric substrate [82]. In such conditions, we can stretch a single neurite by substrate indentation without contacting the cell membrane. We can use whole-cell patch clamp recording to probe the neurite-stretch-induced mechanotransduction within a defined substrate, whose physical and molecular context can be modified to mimic physiologically relevant conditions (Fig. 2). This cell-based technique allows us to decode the ECM-tethered mechanosensitive ion channels. Accordingly, the neurite-stretch-induced action potential was present only in specific subsets of DRG neurons [78]. Further studies on ASIC- mutations would be promising in determining ASIC isoforms as the ECM-tethered mechanosensitive ion channels in sensory nerves.


Neurosensory mechanotransduction through acid-sensing ion channels.

Chen CC, Wong CW - J. Cell. Mol. Med. (2013)

Probing sensory nerve mechanotransduction via localized elastomeric matrix control. Mechanical stretching imposed on a neurite via surface-modified elastomeric matrices. To probe the extracellular matrix (ECM)-tethered mechanotransduction on nerve terminals, neurite-bearing dorsal root ganglia neurons are cultured on an ECM-coated polydimethylsiloxane substrate that mimics a physiologically relevant elastic modulus (10–100 kPa). We can use whole-cell patch clamp recording to measure the electrical responses of neurosensory mechanotransduction on a single neurite by substrate indentation at a location adjacent to the neurite, which will deform the substrate and thus stretch the neurite without contacting it. This figure was modified from [82].
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3823015&req=5

fig02: Probing sensory nerve mechanotransduction via localized elastomeric matrix control. Mechanical stretching imposed on a neurite via surface-modified elastomeric matrices. To probe the extracellular matrix (ECM)-tethered mechanotransduction on nerve terminals, neurite-bearing dorsal root ganglia neurons are cultured on an ECM-coated polydimethylsiloxane substrate that mimics a physiologically relevant elastic modulus (10–100 kPa). We can use whole-cell patch clamp recording to measure the electrical responses of neurosensory mechanotransduction on a single neurite by substrate indentation at a location adjacent to the neurite, which will deform the substrate and thus stretch the neurite without contacting it. This figure was modified from [82].
Mentions: To examine neurosensory mechanotransduction in vitro, the choice of a cell-base technique is critical for testing the hypotheses grounded in mechanobiology [81]. To probe neurosensory mechanotransduction, we recently developed a cell-based method to culture neurite-bearing DRG neurons on an ECM-coated elastomeric substrate [82]. In such conditions, we can stretch a single neurite by substrate indentation without contacting the cell membrane. We can use whole-cell patch clamp recording to probe the neurite-stretch-induced mechanotransduction within a defined substrate, whose physical and molecular context can be modified to mimic physiologically relevant conditions (Fig. 2). This cell-based technique allows us to decode the ECM-tethered mechanosensitive ion channels. Accordingly, the neurite-stretch-induced action potential was present only in specific subsets of DRG neurons [78]. Further studies on ASIC- mutations would be promising in determining ASIC isoforms as the ECM-tethered mechanosensitive ion channels in sensory nerves.

Bottom Line: Thus, ASIC channels alone are not sufficient to reconstruct the path of transducing molecules of mechanically activated channels.The mechanotransducers associated with ASICs need further elucidation.Also we propose a molecular model and a new approach to disclose the molecular mechanism underlying the neurosensory mechanotransduction.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan. chih@ibms.sinica.edu.tw

Show MeSH
Related in: MedlinePlus