Haematopoietic stem cells in spleen have distinct differentiative potential for antigen presenting cells.
Bottom Line: The ability of HSC in spleen to develop into L-DC was indicated by a strong bias in the subset size of these cells over other splenic APC subsets.This type of evidence supports a model whereby spleen represents an important site for haematopoiesis of this distinct DC subset.The conditions under which haematopoiesis of L-DC occurs in spleen, or the progenitors involved, will require further investigation.
Affiliation: Research School of Biology, The Australian National University, Canberra, Australia.Show MeSH
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Mentions: The superior reconstitution potential of HSC in neonatal spleen was long-lasting, providing CD45.1+ donor-type haematopoietic cells in all SPL chimeras tested (3 of 3) for more than 25 weeks (Fig. 2A). As with short-term reconstitution analyses shown in Figure 1B, all long-term SPL chimeras gave almost complete donor (CD45.1+) cell reconstitution. This demonstrates that donor-type neonatal spleen cells (CD45.1+) out-compete an equal number of host-type BM cells (CD45.1−) for haematopoietic reconstitution, under the adoptive transfer protocol used here. Complete (.99%) reconstitution of spleen APC subsets by donor-type spleen HSC was observed in two out of three SPL chimeras analysed after 25 weeks. Only one chimera displayed partial donor reconstitution of APC subsets in spleen at 51 weeks, with proportions of subsets varying from 67% to 96%.
Affiliation: Research School of Biology, The Australian National University, Canberra, Australia.