Telocytes in human epicardium.
Bottom Line: TEM documented that telocytes release shed microvesicles or exocytotic multivesicular bodies in the intercellular space.The human epicardial telocytes have similar phenotype (TEM and IHC) with telocytes located among human working cardiomyocyte.It remains to be established the role(s) of telocytes in cardiac renewing/repair/regeneration processes, and also the pathological aspects induced by their 'functional inhibition', or by their variation in number.
Affiliation: Department of Cellular and Molecular Medicine, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania. LMP@jcmm.orgShow MeSH
Related in: MedlinePlus
Mentions: High levels of CD34 positivity were noted in the epicardium, but due to the vicinity of a small vessels network, the discrimination between telocytes and endothelial cells was sometimes difficult. Telocytes morphology (peculiar telopodes) may be mimicked by the contiguous aspect of endothelial layer in different incidences (Fig. 3A). In order to individualize the CD34 signal from telocytes from that of endothelial cells, double labelling c-kit/CD34 was performed (Fig. 3B). Similarly, double labelling c-kit/vimentin is shown in Fig. 3C. The aspect reveals the telocytes location in the close vicinity of a capillary, an aspect similar with that previously observed in myocardium [36–38, 41]. Figure 4A shows telocytes positive for S-100. Immunolabelling for τ protein was similar with that for S-100 (Fig. 3D) Immunolabelling for CD57 (NK1) was positive for telocytes. However, the results are difficult to interpret because, apparently, the reaction was less specific in cells with prolongations (Fig. 4B).
Affiliation: Department of Cellular and Molecular Medicine, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania. LMP@jcmm.org