The non-anticoagulant heparin-like K5 polysaccharide derivative K5-N,OSepi attenuates myocardial ischaemia/reperfusion injury.
Bottom Line: Chemical and enzymatic modifications of K5 polysaccharide have shown the possibility of producing heparin-like compounds with low anticoagulant activity and strong anti-inflammatory effects.Furthermore, K5-N,OSepi administration attenuated the increase in caspase 3 activity, Bid and Bax activation and ameliorated the decrease in expression of Bcl-2 within the ischaemic myocardium.In conclusion, we demonstrate that the cardioprotective effect of the non-anticoagulant K5 derivative K5-N,OSepi is secondary to a combination of anti-apoptotic and anti-inflammatory effects.
Affiliation: Department of Anatomy, Pharmacology and Forensic Medicine, University of Turin, Turin, Italy.Show MeSH
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Mentions: The results of the computer-assisted morphometry on the I/R hearts stained with nitroblue tetrazolium showed that administration of K5-N,OSepi 15 min. before reperfusion induced a significant reduction in the extension of myocardial damage in comparison with the non-treated rats at both 1 hr and 24 hrs (Fig. 1A). The mean size of ischaemic lesions in the rats treated with K5-N,OSepi was reduced in a dose-dependent manner at 1 hr reperfusion, with a maximum effect at 1 mg/kg (50% reduction). The highest dose of K5-N,OSepi evoked a similar 50% reduction in infarct volume when measured after 24 hrs reperfusion.
Affiliation: Department of Anatomy, Pharmacology and Forensic Medicine, University of Turin, Turin, Italy.