Analysis of molecular mechanisms and anti-tumoural effects of zoledronic acid in breast cancer cells.
Bottom Line: We validated gene expression results of specific transcripts involved in major cellular process by Real Time and Western Blot analysis and we observed inhibition of proliferation and migration through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Matrigel assay.We then focused on changes in the cytoskeletal components as fibronectin 1 (FN1), actin, and anti angiogenic compounds as transforming growth factor-β1 (TGF-β1) and thrombospondin 1 (THBS1).The up-regulation of these products may have an important role in inhibiting proliferation, invasion and angiogenesis mediated by ZOL.
Affiliation: Section of Medical Oncology, Department of Surgical and Oncology, University of Palermo, Palermo, Italy.Show MeSH
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Mentions: Then, the effects of ZOL, on the in vitro invasion of MCF-7 were investigated by Matrigel assays. We observed that cells treated even with only 10 μM of the drug, resulted in a reduction in invasion in a time dependent manner, reaching 60–90% inhibition after 24 hrs (Fig. 5).
Affiliation: Section of Medical Oncology, Department of Surgical and Oncology, University of Palermo, Palermo, Italy.