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Analysis of molecular mechanisms and anti-tumoural effects of zoledronic acid in breast cancer cells.

Insalaco L, Di Gaudio F, Terrasi M, Amodeo V, Caruso S, Corsini LR, Fanale D, Margarese N, Santini D, Bazan V, Russo A - J. Cell. Mol. Med. (2012)

Bottom Line: Microarrays analysis was used to identify, describe and summarize evidence regarding the molecular basis of actions of ZOL and of their possible direct anti-tumour effects.We validated gene expression results of specific transcripts involved in major cellular process by Real Time and Western Blot analysis and we observed inhibition of proliferation and migration through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Matrigel assay.The up-regulation of these products may have an important role in inhibiting proliferation, invasion and angiogenesis mediated by ZOL.

View Article: PubMed Central - PubMed

Affiliation: Section of Medical Oncology, Department of Surgical and Oncology, University of Palermo, Palermo, Italy.

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Related in: MedlinePlus

Effect of ZOL on the mRNA expression and protein levels of FN1 and ACTIN. (A) Effect of ZOL 10 μM on the mRNA expression of FN1 and ACTIN, as quantified by real time PCR in MCF-7 cells. (B) Effect of ZOL on FN1 and ACTIN protein levels. MCF-7 cells were incubated with low concentration of ZOL for different times, and protein expression were examined by Western blot developing with the enhanced chemoluminescence reagent (ECL). Each membrane was also probed with GAPDH to confirm equal loading.
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fig04: Effect of ZOL on the mRNA expression and protein levels of FN1 and ACTIN. (A) Effect of ZOL 10 μM on the mRNA expression of FN1 and ACTIN, as quantified by real time PCR in MCF-7 cells. (B) Effect of ZOL on FN1 and ACTIN protein levels. MCF-7 cells were incubated with low concentration of ZOL for different times, and protein expression were examined by Western blot developing with the enhanced chemoluminescence reagent (ECL). Each membrane was also probed with GAPDH to confirm equal loading.

Mentions: We found that treatment with ZOL induces transcription and protein expression of some matrix and cytoskeletal components, such as Fibronectin and actin, involved in cancer microenvironment. In particular, the up-regulation of gene coding for FN1 shown by microarray (fold change of 1.93) was confirmed by Real Time RT-PCR with a fold change of 2.3 compare with control (Fig. 4A) and mRNA expression of actin, analysed by Real time RT-PCR, showed a fold change of 1.5. Interestingly, a high protein expression is maintained even at longer treatment (at 96 hrs), and with the most activating effect in the protein products, indicating the potential consequences of ZOL treatment on the morphology and cell motility, considered the cellular roles of FN1 and actin as factors that can change the ECM (Fig. 4B).


Analysis of molecular mechanisms and anti-tumoural effects of zoledronic acid in breast cancer cells.

Insalaco L, Di Gaudio F, Terrasi M, Amodeo V, Caruso S, Corsini LR, Fanale D, Margarese N, Santini D, Bazan V, Russo A - J. Cell. Mol. Med. (2012)

Effect of ZOL on the mRNA expression and protein levels of FN1 and ACTIN. (A) Effect of ZOL 10 μM on the mRNA expression of FN1 and ACTIN, as quantified by real time PCR in MCF-7 cells. (B) Effect of ZOL on FN1 and ACTIN protein levels. MCF-7 cells were incubated with low concentration of ZOL for different times, and protein expression were examined by Western blot developing with the enhanced chemoluminescence reagent (ECL). Each membrane was also probed with GAPDH to confirm equal loading.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3822988&req=5

fig04: Effect of ZOL on the mRNA expression and protein levels of FN1 and ACTIN. (A) Effect of ZOL 10 μM on the mRNA expression of FN1 and ACTIN, as quantified by real time PCR in MCF-7 cells. (B) Effect of ZOL on FN1 and ACTIN protein levels. MCF-7 cells were incubated with low concentration of ZOL for different times, and protein expression were examined by Western blot developing with the enhanced chemoluminescence reagent (ECL). Each membrane was also probed with GAPDH to confirm equal loading.
Mentions: We found that treatment with ZOL induces transcription and protein expression of some matrix and cytoskeletal components, such as Fibronectin and actin, involved in cancer microenvironment. In particular, the up-regulation of gene coding for FN1 shown by microarray (fold change of 1.93) was confirmed by Real Time RT-PCR with a fold change of 2.3 compare with control (Fig. 4A) and mRNA expression of actin, analysed by Real time RT-PCR, showed a fold change of 1.5. Interestingly, a high protein expression is maintained even at longer treatment (at 96 hrs), and with the most activating effect in the protein products, indicating the potential consequences of ZOL treatment on the morphology and cell motility, considered the cellular roles of FN1 and actin as factors that can change the ECM (Fig. 4B).

Bottom Line: Microarrays analysis was used to identify, describe and summarize evidence regarding the molecular basis of actions of ZOL and of their possible direct anti-tumour effects.We validated gene expression results of specific transcripts involved in major cellular process by Real Time and Western Blot analysis and we observed inhibition of proliferation and migration through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Matrigel assay.The up-regulation of these products may have an important role in inhibiting proliferation, invasion and angiogenesis mediated by ZOL.

View Article: PubMed Central - PubMed

Affiliation: Section of Medical Oncology, Department of Surgical and Oncology, University of Palermo, Palermo, Italy.

Show MeSH
Related in: MedlinePlus